WDR74_MOUSE
ID WDR74_MOUSE Reviewed; 384 AA.
AC Q8VCG3;
DT 23-NOV-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=WD repeat-containing protein 74;
GN Name=Wdr74;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP FUNCTION, DEVELOPMENTAL STAGE, AND SUBCELLULAR LOCATION.
RX PubMed=21799883; DOI=10.1371/journal.pone.0022516;
RA Maserati M., Walentuk M., Dai X., Holston O., Adams D., Mager J.;
RT "Wdr74 is required for blastocyst formation in the mouse.";
RL PLoS ONE 6:E22516-E22516(2011).
CC -!- FUNCTION: Regulatory protein of the MTREX-exosome complex involved in
CC the synthesis of the 60S ribosomal subunit. Participates in an early
CC cleavage of the pre-rRNA processing pathway in cooperation with NVL (By
CC similarity). Required for blastocyst formation, is necessary for RNA
CC transcription, processing and/or stability during preimplantation
CC development (PubMed:21799883). {ECO:0000250|UniProtKB:Q6RFH5,
CC ECO:0000269|PubMed:21799883}.
CC -!- SUBUNIT: Isoform 1 interacts (through WDR repeats) with NVL; the
CC interaction is independent of RNA or pre-60S ribosome particles.
CC Isoform 2 does not interact with NVL. Interacts with MTREX; the
CC interaction dissociation in a late stage of rRNA synthesis is required
CC for appropriate maturation of pre-60S particles and depends on the
CC ATPase activity of NVL. {ECO:0000250|UniProtKB:Q6RFH5}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q6RFH5}. Nucleus {ECO:0000269|PubMed:21799883}.
CC Note=Nucleolar location depends on active PolI transcription of pre-
CC rRNA. {ECO:0000250|UniProtKB:Q6RFH5}.
CC -!- DEVELOPMENTAL STAGE: Expressed at low levels in MII oocytes and 1-cell
CC embryos and increases through subsequent cleavage stage divisions. The
CC peak of mRNA expression occurs at the morula stage, with a slight
CC decrease in blastocyst embryos. {ECO:0000269|PubMed:21799883}.
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DR EMBL; BC019968; AAH19968.1; -; mRNA.
DR EMBL; AK077521; BAC36842.1; -; mRNA.
DR CCDS; CCDS29541.1; -.
DR RefSeq; NP_598900.1; NM_134139.1.
DR AlphaFoldDB; Q8VCG3; -.
DR SMR; Q8VCG3; -.
DR BioGRID; 223197; 3.
DR STRING; 10090.ENSMUSP00000043315; -.
DR iPTMnet; Q8VCG3; -.
DR PhosphoSitePlus; Q8VCG3; -.
DR EPD; Q8VCG3; -.
DR MaxQB; Q8VCG3; -.
DR PaxDb; Q8VCG3; -.
DR PeptideAtlas; Q8VCG3; -.
DR PRIDE; Q8VCG3; -.
DR ProteomicsDB; 297550; -.
DR Antibodypedia; 28811; 199 antibodies from 24 providers.
DR DNASU; 107071; -.
DR Ensembl; ENSMUST00000049424; ENSMUSP00000043315; ENSMUSG00000042729.
DR GeneID; 107071; -.
DR KEGG; mmu:107071; -.
DR UCSC; uc008gmm.1; mouse.
DR CTD; 54663; -.
DR MGI; MGI:2147427; Wdr74.
DR VEuPathDB; HostDB:ENSMUSG00000042729; -.
DR eggNOG; KOG3881; Eukaryota.
DR GeneTree; ENSGT00390000015119; -.
DR HOGENOM; CLU_033769_2_0_1; -.
DR InParanoid; Q8VCG3; -.
DR OMA; YTGTTMG; -.
DR OrthoDB; 1439033at2759; -.
DR PhylomeDB; Q8VCG3; -.
DR TreeFam; TF314666; -.
DR BioGRID-ORCS; 107071; 27 hits in 75 CRISPR screens.
DR PRO; PR:Q8VCG3; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; Q8VCG3; protein.
DR Bgee; ENSMUSG00000042729; Expressed in morula and 263 other tissues.
DR ExpressionAtlas; Q8VCG3; baseline and differential.
DR Genevisible; Q8VCG3; MM.
DR GO; GO:0000176; C:nuclear exosome (RNase complex); ISO:MGI.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0030687; C:preribosome, large subunit precursor; IBA:GO_Central.
DR GO; GO:0001825; P:blastocyst formation; IMP:MGI.
DR GO; GO:0042273; P:ribosomal large subunit biogenesis; ISS:UniProtKB.
DR GO; GO:0016070; P:RNA metabolic process; IMP:MGI.
DR GO; GO:0006364; P:rRNA processing; ISS:UniProtKB.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR037379; WDR74/Nsa1.
DR PANTHER; PTHR16038; PTHR16038; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 3.
DR SUPFAM; SSF50978; SSF50978; 1.
PE 2: Evidence at transcript level;
KW Methylation; Nucleus; Phosphoprotein; Reference proteome; Repeat;
KW WD repeat.
FT CHAIN 1..384
FT /note="WD repeat-containing protein 74"
FT /id="PRO_0000051429"
FT REPEAT 40..80
FT /note="WD 1"
FT REPEAT 83..122
FT /note="WD 2"
FT REPEAT 128..168
FT /note="WD 3"
FT REPEAT 179..220
FT /note="WD 4"
FT REPEAT 224..266
FT /note="WD 5"
FT REPEAT 267..306
FT /note="WD 6"
FT REGION 320..384
FT /note="Required for nucleolar and nuclear location"
FT /evidence="ECO:0000250|UniProtKB:Q6RFH5"
FT REGION 323..384
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 214
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q6RFH5"
FT MOD_RES 311
FT /note="N6-methyllysine"
FT /evidence="ECO:0000250|UniProtKB:Q6RFH5"
SQ SEQUENCE 384 AA; 42636 MW; 798E84E1C94E9E62 CRC64;
MATASARWNH VWVGTETGIL KGVNLQRKHA ANFTPSGQPR REEAVNALCW GTGGETQILV
GCADRTVRYF NAEEGTFLSQ RYCPGGEGTF RGLAQADGTL ITCVDSGILR VWCENDKEAS
SDPLLELKVG PGVCRMRQDP THTHVVATCG KENALKVWDL QGSEEPVFRA KNVRNDWLDL
RVPIWDQDTQ FLPGSQKLVT CTGYHQVRVY DPVSPQRRPV LEATYGEYPL TAMTLTPEGN
SVIVGNTHGQ LAEIDFRQGR LLGCLKGLAG SVRGLQCHPS KPLLASCGLD RVLRIHRIRN
PRGLEHKVYL KSQLNCLLLS GRDNWEDEPQ EPQEPNQVPS EDTETDELWA SLEAAAKRKL
PDLDQTQGAL QRRKKKKRPG STSP
