WDR75_MOUSE
ID WDR75_MOUSE Reviewed; 830 AA.
AC Q3U821; Q08EC1;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=WD repeat-containing protein 75;
DE AltName: Full=U3 small nucleolar RNA-associated protein 17 homolog;
GN Name=Wdr75; Synonyms=Utp17;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone marrow;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-778, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-778; SER-781 AND TYR-785, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Ribosome biogenesis factor. Involved in nucleolar processing
CC of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA
CC transcription by RNA polymerase I. {ECO:0000250|UniProtKB:Q8IWA0}.
CC -!- SUBUNIT: May be a component of the proposed t-UTP subcomplex of the
CC ribosomal small subunit (SSU) processome containing at least UTP4,
CC WDR43, HEATR1, UTP15, WDR75. {ECO:0000250|UniProtKB:Q8IWA0}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC {ECO:0000250|UniProtKB:Q8IWA0}.
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DR EMBL; AK152412; BAE31198.1; -; mRNA.
DR EMBL; AC109305; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466589; EDK96877.1; -; Genomic_DNA.
DR EMBL; BC117880; AAI17881.1; -; mRNA.
DR EMBL; BC117881; AAI17882.1; -; mRNA.
DR CCDS; CCDS14932.1; -.
DR RefSeq; NP_082875.1; NM_028599.2.
DR AlphaFoldDB; Q3U821; -.
DR SMR; Q3U821; -.
DR STRING; 10090.ENSMUSP00000027139; -.
DR iPTMnet; Q3U821; -.
DR PhosphoSitePlus; Q3U821; -.
DR jPOST; Q3U821; -.
DR MaxQB; Q3U821; -.
DR PaxDb; Q3U821; -.
DR PRIDE; Q3U821; -.
DR ProteomicsDB; 297551; -.
DR Antibodypedia; 52112; 24 antibodies from 12 providers.
DR Ensembl; ENSMUST00000027139; ENSMUSP00000027139; ENSMUSG00000025995.
DR GeneID; 73674; -.
DR KEGG; mmu:73674; -.
DR UCSC; uc007awt.1; mouse.
DR CTD; 84128; -.
DR MGI; MGI:1920924; Wdr75.
DR VEuPathDB; HostDB:ENSMUSG00000025995; -.
DR eggNOG; KOG1963; Eukaryota.
DR GeneTree; ENSGT00390000006303; -.
DR HOGENOM; CLU_005417_2_0_1; -.
DR InParanoid; Q3U821; -.
DR OMA; WILNTRI; -.
DR OrthoDB; 1437651at2759; -.
DR PhylomeDB; Q3U821; -.
DR TreeFam; TF323469; -.
DR Reactome; R-MMU-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR BioGRID-ORCS; 73674; 29 hits in 75 CRISPR screens.
DR ChiTaRS; Wdr75; mouse.
DR PRO; PR:Q3U821; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q3U821; protein.
DR Bgee; ENSMUSG00000025995; Expressed in otic placode and 260 other tissues.
DR ExpressionAtlas; Q3U821; baseline and differential.
DR GO; GO:0005730; C:nucleolus; ISO:MGI.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:2000234; P:positive regulation of rRNA processing; ISO:MGI.
DR GO; GO:0045943; P:positive regulation of transcription by RNA polymerase I; ISO:MGI.
DR GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 3.
DR InterPro; IPR011047; Quinoprotein_ADH-like_supfam.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 3.
DR SMART; SM00320; WD40; 8.
DR SUPFAM; SSF50978; SSF50978; 1.
DR SUPFAM; SSF50998; SSF50998; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE 1: Evidence at protein level;
KW Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; Repeat;
KW Ribosome biogenesis; rRNA processing; Transcription;
KW Transcription regulation; Ubl conjugation; WD repeat.
FT CHAIN 1..830
FT /note="WD repeat-containing protein 75"
FT /id="PRO_0000435727"
FT REPEAT 4..42
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 46..85
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 89..130
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 144..183
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 192..230
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 236..275
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 278..317
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REPEAT 323..361
FT /note="WD 8"
FT /evidence="ECO:0000255"
FT REPEAT 375..422
FT /note="WD 9"
FT /evidence="ECO:0000255"
FT REPEAT 429..473
FT /note="WD 10"
FT /evidence="ECO:0000255"
FT REPEAT 486..524
FT /note="WD 11"
FT /evidence="ECO:0000255"
FT REPEAT 528..568
FT /note="WD 12"
FT /evidence="ECO:0000255"
FT REPEAT 573..610
FT /note="WD 13"
FT /evidence="ECO:0000255"
FT REGION 761..807
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 770..787
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 788..807
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 663
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8IWA0"
FT MOD_RES 671
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8IWA0"
FT MOD_RES 778
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17242355,
FT ECO:0007744|PubMed:21183079"
FT MOD_RES 781
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 785
FT /note="Phosphotyrosine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 811
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q8IWA0"
FT CROSSLNK 426
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q8IWA0"
FT CROSSLNK 675
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q8IWA0"
FT CONFLICT 795
FT /note="Missing (in Ref. 4; AAI17882 and 3; EDK96877)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 830 AA; 94037 MW; 503EBE7BF42630F1 CRC64;
MVEEGVRVVR CGGSRLNFRR AVFSVDSKYI FCVSGDFVKV YSTTTEECVH ILHGHTDLVS
GILVNPSNHL QLYSCSFDGT IKLWDYVDGI LIKTFTIGPK LHAFFIPLHA EDSVFLTISK
EEPDIFQLVS VKLPKSTSQD VEARQLTFVL DYINRSPKCI AFGNEGEYVA AVRDFYLSVY
FFKKKKTCNF TLPSTKNKKN AKNKFTCVAC HPKEDCIASG HMDGKIRLWR NFHSDQKYTY
TCLHWHHDMV MDLAFTVTGT SLLSGGRECV LVEWRDGSEK NKEFLPRLGS SIEHISVSPA
GDLFCTSHSD NKITVIHRNL DASAVIQGLV KDRSISTGLM VDPRTKALVL NGKPGHLQFY
SLQGDKQLYN LDIIQQEYIN DEGLTQTELT KAAFGCSGTW LATVEQRQEN ENELELQMKL
WNYSKKTQGF VLNTKIAMPH DDHITALCFN NAESYEKPIL VTASRDGHFK VWILTDDSDI
YKKAIAWTCD FVGSYHKYQA TNCCFSEDGS LLAVSFEEIV TIWDSQTWEL KCTFCQRAGK
IRHLCFGRLT CSKYLLGTTD NGILCCWNLL SCSIQWSAKL NVRVMEPDPY SDHVAAVAQS
SAGSDLFVFK PSEPRPLYIQ KNVSREEVQW GVFVPRDVPE SFTSETHQWL NRSQFYFLTK
SQSLLTFSTK SPEEKLTPTS KQLLAEESLP TTPFSFILGK HRQQQGAKLT ETSENELVQL
PLTENIPAIT ELLHTPAHVL PSASFLCSLF VNSLLLSKET KSAEEVPDDV DMEGNKESDD
SDEEYDLTEK DKETNNNTDL GEDAIHQLSK SEEKELRKFR KVDYSWLTAL
