WDR76_MOUSE
ID WDR76_MOUSE Reviewed; 622 AA.
AC A6PWY4; A2ASQ8; A4FUV0; Q504Z3; Q9CTV4;
DT 23-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT 21-AUG-2007, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=WD repeat-containing protein 76;
GN Name=Wdr76;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=C57BL/6J; TISSUE=Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION.
RX PubMed=23434322; DOI=10.1016/j.cell.2013.02.004;
RA Spruijt C.G., Gnerlich F., Smits A.H., Pfaffeneder T., Jansen P.W.,
RA Bauer C., Munzel M., Wagner M., Muller M., Khan F., Eberl H.C.,
RA Mensinga A., Brinkman A.B., Lephikov K., Muller U., Walter J., Boelens R.,
RA van Ingen H., Leonhardt H., Carell T., Vermeulen M.;
RT "Dynamic readers for 5-(hydroxy)methylcytosine and its oxidized
RT derivatives.";
RL Cell 152:1146-1159(2013).
CC -!- FUNCTION: Specifically binds 5-hydroxymethylcytosine (5hmC), suggesting
CC that it acts as a specific reader of 5hmC.
CC {ECO:0000269|PubMed:23434322}.
CC -!- SUBUNIT: Interacts with CUL4A and/or CUL4B. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=A6PWY4-1; Sequence=Displayed;
CC Name=2;
CC IsoId=A6PWY4-2; Sequence=VSP_035463;
CC Name=3;
CC IsoId=A6PWY4-3; Sequence=VSP_035464, VSP_035465, VSP_035466;
CC -!- SIMILARITY: Belongs to the WD repeat DDB2/WDR76 family. {ECO:0000305}.
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DR EMBL; AK020004; BAB31961.2; -; mRNA.
DR EMBL; AL928678; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC094676; AAH94676.1; -; mRNA.
DR EMBL; BC117822; AAI17823.1; -; mRNA.
DR CCDS; CCDS38218.1; -. [A6PWY4-2]
DR CCDS; CCDS89546.1; -. [A6PWY4-1]
DR RefSeq; NP_001277915.1; NM_001290986.1. [A6PWY4-2]
DR RefSeq; NP_001277916.1; NM_001290987.1.
DR RefSeq; NP_084510.2; NM_030234.2. [A6PWY4-2]
DR RefSeq; XP_006499556.1; XM_006499493.3.
DR AlphaFoldDB; A6PWY4; -.
DR SMR; A6PWY4; -.
DR BioGRID; 232333; 7.
DR DIP; DIP-59892N; -.
DR IntAct; A6PWY4; 2.
DR STRING; 10090.ENSMUSP00000028676; -.
DR iPTMnet; A6PWY4; -.
DR PhosphoSitePlus; A6PWY4; -.
DR EPD; A6PWY4; -.
DR MaxQB; A6PWY4; -.
DR PaxDb; A6PWY4; -.
DR PeptideAtlas; A6PWY4; -.
DR PRIDE; A6PWY4; -.
DR ProteomicsDB; 299751; -. [A6PWY4-1]
DR ProteomicsDB; 299752; -. [A6PWY4-2]
DR ProteomicsDB; 299753; -. [A6PWY4-3]
DR Antibodypedia; 52296; 77 antibodies from 21 providers.
DR Ensembl; ENSMUST00000028676; ENSMUSP00000028676; ENSMUSG00000027242. [A6PWY4-2]
DR Ensembl; ENSMUST00000110602; ENSMUSP00000106232; ENSMUSG00000027242. [A6PWY4-2]
DR Ensembl; ENSMUST00000110603; ENSMUSP00000106234; ENSMUSG00000027242. [A6PWY4-1]
DR GeneID; 241627; -.
DR KEGG; mmu:241627; -.
DR UCSC; uc008lzi.1; mouse. [A6PWY4-2]
DR UCSC; uc008lzj.1; mouse. [A6PWY4-1]
DR CTD; 79968; -.
DR MGI; MGI:1926186; Wdr76.
DR VEuPathDB; HostDB:ENSMUSG00000027242; -.
DR eggNOG; KOG4328; Eukaryota.
DR GeneTree; ENSGT00510000048144; -.
DR HOGENOM; CLU_017019_0_0_1; -.
DR InParanoid; A6PWY4; -.
DR OMA; TVVRHNC; -.
DR OrthoDB; 753973at2759; -.
DR PhylomeDB; A6PWY4; -.
DR TreeFam; TF314788; -.
DR BioGRID-ORCS; 241627; 1 hit in 60 CRISPR screens.
DR ChiTaRS; Wdr76; mouse.
DR PRO; PR:A6PWY4; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; A6PWY4; protein.
DR Bgee; ENSMUSG00000027242; Expressed in animal zygote and 142 other tissues.
DR ExpressionAtlas; A6PWY4; baseline and differential.
DR Genevisible; A6PWY4; MM.
DR GO; GO:0000792; C:heterochromatin; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0090734; C:site of DNA damage; ISS:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IBA:GO_Central.
DR GO; GO:0019899; F:enzyme binding; ISO:MGI.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; ISS:UniProtKB.
DR GO; GO:2000001; P:regulation of DNA damage checkpoint; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR033052; Cmr1/WDR76.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR14773:SF0; PTHR14773:SF0; 1.
DR SMART; SM00320; WD40; 6.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Reference proteome; Repeat; WD repeat.
FT CHAIN 1..622
FT /note="WD repeat-containing protein 76"
FT /id="PRO_0000351092"
FT REPEAT 310..351
FT /note="WD 1"
FT REPEAT 356..398
FT /note="WD 2"
FT REPEAT 400..438
FT /note="WD 3"
FT REPEAT 443..482
FT /note="WD 4"
FT REPEAT 490..530
FT /note="WD 5"
FT REPEAT 540..584
FT /note="WD 6"
FT REPEAT 587..622
FT /note="WD 7"
FT REGION 75..94
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 120..155
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 189..209
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 120..141
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..98
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_035463"
FT VAR_SEQ 181
FT /note="E -> ETLVSLLVGVACCPSLN (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_035464"
FT VAR_SEQ 397..402
FT /note="YRNEGN -> GSDQEA (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_035465"
FT VAR_SEQ 403..622
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_035466"
FT CONFLICT 335
FT /note="S -> Y (in Ref. 1; BAB31961)"
FT /evidence="ECO:0000305"
FT CONFLICT 535
FT /note="I -> V (in Ref. 3; AAI17823)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 622 AA; 69007 MW; C6F28ED51BAC60AD CRC64;
MSGSKAESEE KAGSKQCPLV QVNEYKENEH IAYTSLRPIQ ITTLRKTAKV YLYPFSLSNS
KLGLLKLSKS PVVNNSSKSV VHKKKDRKKT RRKVLTSKMK ALSSKADSLL LKSSVDAYTE
STRLGPKRTS DSATLSVDAE SSDEDSAPGL DDFSGLSPYE RKRLRNIREN ANFFASLQLA
ESAARLRGMI KKRESPESKR KRPKKKENEI GCRRSMRLLK VDPLGVSLPA SPTQPTLVEE
EENPLLPPGP LEMIPENQDD SSELLKASLK TWAEMSQTSN EKTKKGLSSI KSYKANLSGM
VISEATVRKV TKGAISSVAL HPSEVRTLVA AGAKSGQIGL WDLTQQSEDA MYVFYAHSRY
VSCLSFSPTN PAHLLSLSYD GTLRCGDFSS AVFEEVYRNE GNSPSSFDFL NDSSSLLVGH
WDGHLSLVDR RTPGTSYEKF FNSSLEKIRT VHVHPLSRQY FVTAGLRDVH VYDARFLKSR
GSQPLISLTE HSKSIASAYF SPVTGNRVVT TCADCKLRVF DSSSISSQLP LLSTIRHNTV
TGRWLTRFQA VWDPKQEDCF IVGSMDHPRR VEVFHESGKN VHSLWGECLV SVCSLSAVHP
TRYILAGGNS SGKLHVFMHQ ET
