WDR83_HUMAN
ID WDR83_HUMAN Reviewed; 315 AA.
AC Q9BRX9; B2RAF1; Q53FT6;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=WD repeat domain-containing protein 83;
DE AltName: Full=Mitogen-activated protein kinase organizer 1;
DE Short=MAPK organizer 1;
GN Name=WDR83; Synonyms=MORG1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney proximal tubule;
RA Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA Tanaka A., Yokoyama S.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Muscle;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND IDENTIFICATION IN THE SPLICEOSOMAL
RP C COMPLEX.
RX PubMed=11991638; DOI=10.1017/s1355838202021088;
RA Jurica M.S., Licklider L.J., Gygi S.P., Grigorieff N., Moore M.J.;
RT "Purification and characterization of native spliceosomes suitable for
RT three-dimensional structural analysis.";
RL RNA 8:426-439(2002).
CC -!- FUNCTION: Molecular scaffold protein for various multimeric protein
CC complexes. Acts as a module in the assembly of a multicomponent
CC scaffold for the ERK pathway, linking ERK responses to specific
CC agonists. At low concentrations it enhances ERK activation, whereas
CC high concentrations lead to the inhibition of ERK activation. Also
CC involved in response to hypoxia by acting as a negative regulator of
CC HIF1A/HIF-1-alpha via its interaction with EGLN3/PHD3. May promote
CC degradation of HIF1A. May act by recruiting signaling complexes to a
CC specific upstream activator (By similarity). May also be involved in
CC pre-mRNA splicing. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with EGLN3/PHD3. Interacts with ERK signaling
CC proteins MAP2K1/MEK1, MAP2K2/MEK2, LAMTOR3, ARAF/Raf-1, MAPK1/ERK2 and
CC MAPK3/ERK1 (By similarity). Identified in the spliceosome C complex.
CC {ECO:0000250, ECO:0000269|PubMed:11991638}.
CC -!- INTERACTION:
CC Q9BRX9; P54253: ATXN1; NbExp=6; IntAct=EBI-7705033, EBI-930964;
CC Q9BRX9; P41182: BCL6; NbExp=3; IntAct=EBI-7705033, EBI-765407;
CC Q9BRX9; Q96A83-2: COL26A1; NbExp=3; IntAct=EBI-7705033, EBI-21553822;
CC Q9BRX9; P46108: CRK; NbExp=2; IntAct=EBI-7705033, EBI-886;
CC Q9BRX9; G5E9A7: DMWD; NbExp=3; IntAct=EBI-7705033, EBI-10976677;
CC Q9BRX9; O75190-2: DNAJB6; NbExp=3; IntAct=EBI-7705033, EBI-12593112;
CC Q9BRX9; O14645: DNALI1; NbExp=3; IntAct=EBI-7705033, EBI-395638;
CC Q9BRX9; Q9H5Z6-2: FAM124B; NbExp=3; IntAct=EBI-7705033, EBI-11986315;
CC Q9BRX9; P21333-2: FLNA; NbExp=3; IntAct=EBI-7705033, EBI-9641086;
CC Q9BRX9; O43559: FRS3; NbExp=3; IntAct=EBI-7705033, EBI-725515;
CC Q9BRX9; P14136: GFAP; NbExp=3; IntAct=EBI-7705033, EBI-744302;
CC Q9BRX9; Q9Y223-2: GNE; NbExp=3; IntAct=EBI-7705033, EBI-11975289;
CC Q9BRX9; P49639: HOXA1; NbExp=3; IntAct=EBI-7705033, EBI-740785;
CC Q9BRX9; P04792: HSPB1; NbExp=4; IntAct=EBI-7705033, EBI-352682;
CC Q9BRX9; Q8WXH2: JPH3; NbExp=3; IntAct=EBI-7705033, EBI-1055254;
CC Q9BRX9; Q7L273: KCTD9; NbExp=3; IntAct=EBI-7705033, EBI-4397613;
CC Q9BRX9; Q14145: KEAP1; NbExp=7; IntAct=EBI-7705033, EBI-751001;
CC Q9BRX9; O60333-2: KIF1B; NbExp=3; IntAct=EBI-7705033, EBI-10975473;
CC Q9BRX9; O14901: KLF11; NbExp=3; IntAct=EBI-7705033, EBI-948266;
CC Q9BRX9; Q17RB8: LONRF1; NbExp=3; IntAct=EBI-7705033, EBI-2341787;
CC Q9BRX9; P19404: NDUFV2; NbExp=3; IntAct=EBI-7705033, EBI-713665;
CC Q9BRX9; Q86UR1-2: NOXA1; NbExp=3; IntAct=EBI-7705033, EBI-12025760;
CC Q9BRX9; D3DTS7: PMP22; NbExp=3; IntAct=EBI-7705033, EBI-25882629;
CC Q9BRX9; Q9Y3C6: PPIL1; NbExp=2; IntAct=EBI-7705033, EBI-2557649;
CC Q9BRX9; O43741: PRKAB2; NbExp=3; IntAct=EBI-7705033, EBI-1053424;
CC Q9BRX9; Q6P2Q9: PRPF8; NbExp=2; IntAct=EBI-7705033, EBI-538479;
CC Q9BRX9; P60891: PRPS1; NbExp=3; IntAct=EBI-7705033, EBI-749195;
CC Q9BRX9; Q9Y3C5: RNF11; NbExp=3; IntAct=EBI-7705033, EBI-396669;
CC Q9BRX9; Q15427: SF3B4; NbExp=2; IntAct=EBI-7705033, EBI-348469;
CC Q9BRX9; P09234: SNRPC; NbExp=2; IntAct=EBI-7705033, EBI-766589;
CC Q9BRX9; Q7Z699: SPRED1; NbExp=3; IntAct=EBI-7705033, EBI-5235340;
CC Q9BRX9; Q13148: TARDBP; NbExp=6; IntAct=EBI-7705033, EBI-372899;
CC Q9BRX9; O43711: TLX3; NbExp=3; IntAct=EBI-7705033, EBI-3939165;
CC Q9BRX9; P02766: TTR; NbExp=3; IntAct=EBI-7705033, EBI-711909;
CC Q9BRX9; Q9BZL1: UBL5; NbExp=2; IntAct=EBI-7705033, EBI-607755;
CC Q9BRX9; Q08AM6: VAC14; NbExp=3; IntAct=EBI-7705033, EBI-2107455;
CC Q9BRX9; O76024: WFS1; NbExp=3; IntAct=EBI-7705033, EBI-720609;
CC Q9BRX9; P36508: ZNF76; NbExp=3; IntAct=EBI-7705033, EBI-7254550;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC Note=Predominantly cytoplasmic. Partially nuclear. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the WD repeat MORG1 family. {ECO:0000305}.
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DR EMBL; AK223195; BAD96915.1; -; mRNA.
DR EMBL; AK314164; BAG36848.1; -; mRNA.
DR EMBL; CH471106; EAW84282.1; -; Genomic_DNA.
DR EMBL; BC005870; AAH05870.1; -; mRNA.
DR CCDS; CCDS12275.1; -.
DR RefSeq; NP_001093207.1; NM_001099737.2.
DR RefSeq; NP_115708.1; NM_032332.3.
DR AlphaFoldDB; Q9BRX9; -.
DR SMR; Q9BRX9; -.
DR BioGRID; 124019; 190.
DR CORUM; Q9BRX9; -.
DR IntAct; Q9BRX9; 154.
DR MINT; Q9BRX9; -.
DR STRING; 9606.ENSP00000402653; -.
DR iPTMnet; Q9BRX9; -.
DR PhosphoSitePlus; Q9BRX9; -.
DR BioMuta; WDR83; -.
DR DMDM; 74761224; -.
DR EPD; Q9BRX9; -.
DR jPOST; Q9BRX9; -.
DR MassIVE; Q9BRX9; -.
DR MaxQB; Q9BRX9; -.
DR PaxDb; Q9BRX9; -.
DR PeptideAtlas; Q9BRX9; -.
DR PRIDE; Q9BRX9; -.
DR ProteomicsDB; 78851; -.
DR Antibodypedia; 26076; 221 antibodies from 29 providers.
DR DNASU; 84292; -.
DR Ensembl; ENST00000418543.8; ENSP00000402653.3; ENSG00000123154.12.
DR GeneID; 84292; -.
DR KEGG; hsa:84292; -.
DR MANE-Select; ENST00000418543.8; ENSP00000402653.3; NM_001099737.3; NP_001093207.1.
DR UCSC; uc002mue.5; human.
DR CTD; 84292; -.
DR DisGeNET; 84292; -.
DR GeneCards; WDR83; -.
DR HGNC; HGNC:32672; WDR83.
DR HPA; ENSG00000123154; Low tissue specificity.
DR neXtProt; NX_Q9BRX9; -.
DR OpenTargets; ENSG00000123154; -.
DR PharmGKB; PA165394765; -.
DR VEuPathDB; HostDB:ENSG00000123154; -.
DR eggNOG; KOG0316; Eukaryota.
DR GeneTree; ENSGT00940000159016; -.
DR InParanoid; Q9BRX9; -.
DR OMA; MCWDIRT; -.
DR OrthoDB; 1090342at2759; -.
DR PhylomeDB; Q9BRX9; -.
DR TreeFam; TF314828; -.
DR PathwayCommons; Q9BRX9; -.
DR Reactome; R-HSA-5674135; MAP2K and MAPK activation.
DR SignaLink; Q9BRX9; -.
DR SIGNOR; Q9BRX9; -.
DR BioGRID-ORCS; 84292; 308 hits in 1088 CRISPR screens.
DR ChiTaRS; WDR83; human.
DR GenomeRNAi; 84292; -.
DR Pharos; Q9BRX9; Tbio.
DR PRO; PR:Q9BRX9; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q9BRX9; protein.
DR Bgee; ENSG00000123154; Expressed in right hemisphere of cerebellum and 117 other tissues.
DR ExpressionAtlas; Q9BRX9; baseline and differential.
DR Genevisible; Q9BRX9; HS.
DR GO; GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
DR GO; GO:0010008; C:endosome membrane; TAS:Reactome.
DR GO; GO:0005681; C:spliceosomal complex; IDA:UniProtKB.
DR GO; GO:0090594; P:inflammatory response to wounding; IEA:Ensembl.
DR GO; GO:0000398; P:mRNA splicing, via spliceosome; IDA:UniProtKB.
DR GO; GO:0043122; P:regulation of I-kappaB kinase/NF-kappaB signaling; IEA:Ensembl.
DR GO; GO:0001666; P:response to hypoxia; IEA:Ensembl.
DR GO; GO:0032496; P:response to lipopolysaccharide; IEA:Ensembl.
DR GO; GO:0000375; P:RNA splicing, via transesterification reactions; IDA:UniProtKB.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR Pfam; PF00400; WD40; 4.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 3.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; mRNA processing; mRNA splicing; Nucleus; Reference proteome;
KW Repeat; Spliceosome; WD repeat.
FT CHAIN 1..315
FT /note="WD repeat domain-containing protein 83"
FT /id="PRO_0000235263"
FT REPEAT 23..62
FT /note="WD 1"
FT REPEAT 65..104
FT /note="WD 2"
FT REPEAT 107..146
FT /note="WD 3"
FT REPEAT 151..188
FT /note="WD 4"
FT REPEAT 190..228
FT /note="WD 5"
FT REPEAT 231..272
FT /note="WD 6"
FT REPEAT 275..313
FT /note="WD 7"
FT VARIANT 278
FT /note="G -> S (in dbSNP:rs34373915)"
FT /id="VAR_053410"
FT VARIANT 304
FT /note="R -> Q (in dbSNP:rs35092999)"
FT /id="VAR_053411"
FT CONFLICT 162
FT /note="S -> L (in Ref. 2; BAD96915)"
FT /evidence="ECO:0000305"
FT CONFLICT 278
FT /note="G -> D (in Ref. 2; BAD96915)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 315 AA; 34343 MW; CF5F59C1FAA432FE CRC64;
MAFPEPKPRP PELPQKRLKT LDCGQGAVRA VRFNVDGNYC LTCGSDKTLK LWNPLRGTLL
RTYSGHGYEV LDAAGSFDNS SLCSGGGDKA VVLWDVASGQ VVRKFRGHAG KVNTVQFNEE
ATVILSGSID SSIRCWDCRS RRPEPVQTLD EARDGVSSVK VSDHEILAGS VDGRVRRYDL
RMGQLFSDYV GSPITCTCFS RDGQCTLVSS LDSTLRLLDK DTGELLGEYK GHKNQEYKLD
CCLSERDTHV VSCSEDGKVF FWDLVEGALA LALPVGSGVV QSLAYHPTEP CLLTAMGGSV
QCWREEAYEA EDGAG
