WDR91_MOUSE
ID WDR91_MOUSE Reviewed; 748 AA.
AC Q7TMQ7; Q80XN1;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=WD repeat-containing protein 91 {ECO:0000312|MGI:MGI:2141558};
GN Name=Wdr91 {ECO:0000312|MGI:MGI:2141558};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N, and FVB/N-3; TISSUE=Liver, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=17242355; DOI=10.1073/pnas.0609836104;
RA Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P.;
RT "Large-scale phosphorylation analysis of mouse liver.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-294, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION.
RX PubMed=27346680; DOI=10.1016/j.stemcr.2016.05.013;
RA Wang S., Wang X., Ma L., Lin X., Zhang D., Li Z., Wu Y., Zheng C., Feng X.,
RA Liao S., Feng Y., Chen J., Hu X., Wang M., Han C.;
RT "Retinoic Acid is sufficient for the in vitro induction of mouse
RT spermatocytes.";
RL Stem Cell Reports 7:80-94(2016).
CC -!- FUNCTION: Functions as a negative regulator of the PI3 kinase/PI3K
CC activity associated with endosomal membranes via BECN1, a core subunit
CC of the PI3K complex. By modifying the phosphatidylinositol 3-
CC phosphate/PtdInsP3 content of endosomal membranes may regulate endosome
CC fusion, recycling, sorting and early to late endosome transport. It is
CC for instance, required for the delivery of cargos like BST2/tetherin
CC from early to late endosome and thereby participates indirectly to
CC their degradation by the lysosome (By similarity). May play a role in
CC meiosis (PubMed:27346680). {ECO:0000250|UniProtKB:A4D1P6,
CC ECO:0000269|PubMed:27346680}.
CC -!- SUBUNIT: Interacts with WDR81; involved in early to late endosome cargo
CC transport. Interacts with BECN1; negatively regulates the PI3
CC kinase/PI3K activity associated with endosomal membranes.
CC {ECO:0000250|UniProtKB:A4D1P6}.
CC -!- SUBCELLULAR LOCATION: Early endosome membrane
CC {ECO:0000250|UniProtKB:A4D1P6}; Peripheral membrane protein
CC {ECO:0000250|UniProtKB:A4D1P6}. Late endosome membrane
CC {ECO:0000250|UniProtKB:A4D1P6}.
CC -!- SIMILARITY: Belongs to the WD repeat WDR91 family. {ECO:0000305}.
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DR EMBL; BC043682; AAH43682.1; -; mRNA.
DR EMBL; BC055046; AAH55046.1; -; mRNA.
DR CCDS; CCDS39457.1; -.
DR RefSeq; NP_001013384.1; NM_001013366.1.
DR AlphaFoldDB; Q7TMQ7; -.
DR SMR; Q7TMQ7; -.
DR BioGRID; 221616; 2.
DR IntAct; Q7TMQ7; 1.
DR STRING; 10090.ENSMUSP00000079974; -.
DR iPTMnet; Q7TMQ7; -.
DR PhosphoSitePlus; Q7TMQ7; -.
DR EPD; Q7TMQ7; -.
DR jPOST; Q7TMQ7; -.
DR MaxQB; Q7TMQ7; -.
DR PaxDb; Q7TMQ7; -.
DR PeptideAtlas; Q7TMQ7; -.
DR PRIDE; Q7TMQ7; -.
DR ProteomicsDB; 297554; -.
DR Antibodypedia; 18119; 98 antibodies from 15 providers.
DR DNASU; 101240; -.
DR Ensembl; ENSMUST00000081214; ENSMUSP00000079974; ENSMUSG00000058486.
DR GeneID; 101240; -.
DR KEGG; mmu:101240; -.
DR UCSC; uc009bhz.1; mouse.
DR CTD; 29062; -.
DR MGI; MGI:2141558; Wdr91.
DR VEuPathDB; HostDB:ENSMUSG00000058486; -.
DR eggNOG; KOG1333; Eukaryota.
DR GeneTree; ENSGT00390000001566; -.
DR HOGENOM; CLU_022078_0_0_1; -.
DR InParanoid; Q7TMQ7; -.
DR OMA; VLFQCML; -.
DR OrthoDB; 321271at2759; -.
DR PhylomeDB; Q7TMQ7; -.
DR TreeFam; TF317339; -.
DR Reactome; R-MMU-9013148; CDC42 GTPase cycle.
DR BioGRID-ORCS; 101240; 5 hits in 75 CRISPR screens.
DR ChiTaRS; Wdr91; mouse.
DR PRO; PR:Q7TMQ7; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q7TMQ7; protein.
DR Bgee; ENSMUSG00000058486; Expressed in animal zygote and 216 other tissues.
DR ExpressionAtlas; Q7TMQ7; baseline and differential.
DR Genevisible; Q7TMQ7; MM.
DR GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR GO; GO:0031901; C:early endosome membrane; ISS:UniProtKB.
DR GO; GO:0031313; C:extrinsic component of endosome membrane; ISS:UniProtKB.
DR GO; GO:0031902; C:late endosome membrane; ISS:UniProtKB.
DR GO; GO:0035014; F:phosphatidylinositol 3-kinase regulator activity; ISS:UniProtKB.
DR GO; GO:0045022; P:early endosome to late endosome transport; ISS:UniProtKB.
DR GO; GO:0043551; P:regulation of phosphatidylinositol 3-kinase activity; ISS:UniProtKB.
DR GO; GO:0042176; P:regulation of protein catabolic process; ISO:MGI.
DR Gene3D; 2.130.10.10; -; 2.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR039724; WDR91.
DR PANTHER; PTHR13083; PTHR13083; 1.
DR Pfam; PF00400; WD40; 1.
DR SMART; SM00320; WD40; 5.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS50082; WD_REPEATS_2; 1.
DR PROSITE; PS50294; WD_REPEATS_REGION; 2.
PE 1: Evidence at protein level;
KW Coiled coil; Endosome; Membrane; Phosphoprotein; Reference proteome;
KW Repeat; WD repeat.
FT CHAIN 1..748
FT /note="WD repeat-containing protein 91"
FT /id="PRO_0000295747"
FT REPEAT 407..446
FT /note="WD 1"
FT REPEAT 449..489
FT /note="WD 2"
FT REPEAT 512..556
FT /note="WD 3"
FT REPEAT 561..600
FT /note="WD 4"
FT REPEAT 603..642
FT /note="WD 5"
FT REPEAT 665..703
FT /note="WD 6"
FT REPEAT 710..748
FT /note="WD 7"
FT REGION 266..368
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 183..228
FT /evidence="ECO:0000255"
FT COMPBIAS 266..303
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 329..344
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 257
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:A4D1P6"
FT MOD_RES 289
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:A4D1P6"
FT MOD_RES 294
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319"
FT CONFLICT 590
FT /note="F -> L (in Ref. 1; AAH43682)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 748 AA; 83421 MW; 3973DC3AD76DFA13 CRC64;
MAEAVERTDE LVREYLLFRG FTHTLRQLDA EIKADKEKGF RVDKIVDQLQ QLMQVYDLAA
LRDYWSYLER RLFSRLEDIY RPTINKLKTS LFRFYLVYTI QTNRNDKAQE FFAKQATELQ
NQAEWKDWFV LPFLPSPDTN PTFATYFSRQ WADTFIISLH NFLSVLFQCM PVPVILNFDA
ECQRTNQVQE ENEVLRQKLF ALQAEIHRLK KEEQQQEEEA AALVQHKLPP YVSSMDRLGD
SELALVCSQR PASLSQSPRV GFLSSLLPQS KKSPSRLSPA QGPPQAQSSA KKDSFSSQAT
KGKDSVPGAK DGKSLLSGPV PGEASWTHQR QRRLQDHGKE RRELLSTSSS QSQCAERKPE
VSGAEAEPCL ELHMGPVEVL ARVSTAGSEG DRPEQPFIVL SQEEYGEHHS SIMHCRVDCS
GRRVASLDVD GVIKVWSFNP IMQTKASSIS KSPLLSLEWA TKRDRLLLLG SGVGTVRLYD
TEAKKNLCEI NINDDMPRIL SLACSPNGAS FVCSAAAPSL TSQTDSSAPD IGSKGMNQVP
GKLLLWDTKT MKQQLQFSLD PEPIAINCTA FNHNGNLLVT GAADGVIRLF DMQQHECAMS
WKAHCGEVYS VEFSCDENAV YSIGEDRKFI QWNIHKSGLK VSESNLPSDA TGPFVLSGYS
GYKQVQVPRG RLFAFDSEGN YMLTCSATGG LIYKLGSEEK VLENCLSLGG HRAPVVTVDW
STAMDCGTCL TASMDGKIKL TTLLAHKL
