WECB_YERPE
ID WECB_YERPE Reviewed; 376 AA.
AC Q8ZAE3; Q0WAE6;
DT 05-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 05-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=UDP-N-acetylglucosamine 2-epimerase {ECO:0000255|HAMAP-Rule:MF_02028};
DE EC=5.1.3.14 {ECO:0000255|HAMAP-Rule:MF_02028};
DE AltName: Full=UDP-GlcNAc-2-epimerase {ECO:0000255|HAMAP-Rule:MF_02028};
GN Name=wecB {ECO:0000255|HAMAP-Rule:MF_02028}; Synonyms=rffE;
GN OrderedLocusNames=YPO3864, y0364, YP_3181;
OS Yersinia pestis.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Yersinia.
OX NCBI_TaxID=632;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CO-92 / Biovar Orientalis;
RX PubMed=11586360; DOI=10.1038/35097083;
RA Parkhill J., Wren B.W., Thomson N.R., Titball R.W., Holden M.T.G.,
RA Prentice M.B., Sebaihia M., James K.D., Churcher C.M., Mungall K.L.,
RA Baker S., Basham D., Bentley S.D., Brooks K., Cerdeno-Tarraga A.-M.,
RA Chillingworth T., Cronin A., Davies R.M., Davis P., Dougan G., Feltwell T.,
RA Hamlin N., Holroyd S., Jagels K., Karlyshev A.V., Leather S., Moule S.,
RA Oyston P.C.F., Quail M.A., Rutherford K.M., Simmonds M., Skelton J.,
RA Stevens K., Whitehead S., Barrell B.G.;
RT "Genome sequence of Yersinia pestis, the causative agent of plague.";
RL Nature 413:523-527(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=KIM10+ / Biovar Mediaevalis;
RX PubMed=12142430; DOI=10.1128/jb.184.16.4601-4611.2002;
RA Deng W., Burland V., Plunkett G. III, Boutin A., Mayhew G.F., Liss P.,
RA Perna N.T., Rose D.J., Mau B., Zhou S., Schwartz D.C., Fetherston J.D.,
RA Lindler L.E., Brubaker R.R., Plano G.V., Straley S.C., McDonough K.A.,
RA Nilles M.L., Matson J.S., Blattner F.R., Perry R.D.;
RT "Genome sequence of Yersinia pestis KIM.";
RL J. Bacteriol. 184:4601-4611(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=91001 / Biovar Mediaevalis;
RX PubMed=15368893; DOI=10.1093/dnares/11.3.179;
RA Song Y., Tong Z., Wang J., Wang L., Guo Z., Han Y., Zhang J., Pei D.,
RA Zhou D., Qin H., Pang X., Han Y., Zhai J., Li M., Cui B., Qi Z., Jin L.,
RA Dai R., Chen F., Li S., Ye C., Du Z., Lin W., Wang J., Yu J., Yang H.,
RA Wang J., Huang P., Yang R.;
RT "Complete genome sequence of Yersinia pestis strain 91001, an isolate
RT avirulent to humans.";
RL DNA Res. 11:179-197(2004).
CC -!- FUNCTION: Catalyzes the reversible epimerization at C-2 of UDP-N-
CC acetylglucosamine (UDP-GlcNAc) and thereby provides bacteria with UDP-
CC N-acetylmannosamine (UDP-ManNAc), the activated donor of ManNAc
CC residues. {ECO:0000255|HAMAP-Rule:MF_02028}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=UDP-N-acetyl-alpha-D-glucosamine = UDP-N-acetyl-alpha-D-
CC mannosamine; Xref=Rhea:RHEA:17213, ChEBI:CHEBI:57705,
CC ChEBI:CHEBI:68623; EC=5.1.3.14; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_02028};
CC -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_02028}.
CC -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_02028}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_02028}.
CC -!- SIMILARITY: Belongs to the UDP-N-acetylglucosamine 2-epimerase family.
CC {ECO:0000255|HAMAP-Rule:MF_02028}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM83953.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAS63349.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AL590842; CAL22451.1; -; Genomic_DNA.
DR EMBL; AE009952; AAM83953.1; ALT_INIT; Genomic_DNA.
DR EMBL; AE017042; AAS63349.1; ALT_INIT; Genomic_DNA.
DR PIR; AH0470; AH0470.
DR RefSeq; WP_002211986.1; NZ_WHLN01000069.1.
DR RefSeq; YP_002348742.1; NC_003143.1.
DR AlphaFoldDB; Q8ZAE3; -.
DR SMR; Q8ZAE3; -.
DR STRING; 214092.YPO3864; -.
DR PaxDb; Q8ZAE3; -.
DR DNASU; 1145311; -.
DR EnsemblBacteria; AAM83953; AAM83953; y0364.
DR EnsemblBacteria; AAS63349; AAS63349; YP_3181.
DR GeneID; 57974839; -.
DR KEGG; ype:YPO3864; -.
DR KEGG; ypk:y0364; -.
DR KEGG; ypm:YP_3181; -.
DR PATRIC; fig|214092.21.peg.4390; -.
DR eggNOG; COG0381; Bacteria.
DR HOGENOM; CLU_041674_1_0_6; -.
DR OMA; RYNTERP; -.
DR UniPathway; UPA00566; -.
DR Proteomes; UP000000815; Chromosome.
DR Proteomes; UP000001019; Chromosome.
DR Proteomes; UP000002490; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0008761; F:UDP-N-acetylglucosamine 2-epimerase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR HAMAP; MF_02028; WecB_RffE; 1.
DR InterPro; IPR003331; UDP_GlcNAc_Epimerase_2_dom.
DR InterPro; IPR032892; WecB.
DR InterPro; IPR029767; WecB-like.
DR PANTHER; PTHR43174; PTHR43174; 1.
DR Pfam; PF02350; Epimerase_2; 1.
DR TIGRFAMs; TIGR00236; wecB; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Isomerase; Reference proteome.
FT CHAIN 1..376
FT /note="UDP-N-acetylglucosamine 2-epimerase"
FT /id="PRO_0000208531"
FT BINDING 10
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02028"
FT BINDING 15
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02028"
FT BINDING 95
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02028"
FT BINDING 117
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02028"
FT BINDING 213
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02028"
FT BINDING 271
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02028"
FT BINDING 276
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02028"
FT BINDING 290..292
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02028"
FT BINDING 296
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02028"
FT BINDING 313
FT /ligand="substrate"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_02028"
SQ SEQUENCE 376 AA; 42379 MW; 0F9BE03C292DDD02 CRC64;
MKVLTVFGTR PEAIKMAPLV HALAQDDAFE SRVCVTAQHR EMLDQVLRLF EIQPDYDLDI
MRPGQGLTEI TCRILEGLKP VLEEFKPDVI LVHGDTTTTL SASLAGFYHR IPVGHVEAGL
RTGDLYSPWP EEANRQLTGH LAMYHFAPTE NSRQNLLREW VPENRIFVTG NTVIDALFWV
RDRVMNTPDL RANLAQRYAF LDTNKKMILV TGHRRESFGG GFERICSALA EIARKHPEVQ
VVYPVHLNPN VSEPVNRILK GIDNIILIDP QDYLPFVYLM NHAYLILTDS GGIQEEAPSL
GKPVLVMRDT TERPEAVDSG TVLLVGTNIN KIVDAVTRLL TDETAYHQMT RAHNPYGDGY
ACQRILKALK NHQVTL
