WECF_ECOLI
ID WECF_ECOLI Reviewed; 359 AA.
AC P56258; Q2M8A1; Q93DX7;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 05-MAR-2002, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferase;
DE EC=2.4.1.325;
DE AltName: Full=4-alpha-L-fucosyltransferase;
DE AltName: Full=TDP-Fuc4NAc:lipid II Fuc4NAc transferase;
DE Short=Fuc4NAc transferase;
GN Name=wecF; Synonyms=rffT, yifM; OrderedLocusNames=b4481, JW5596;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=11673418; DOI=10.1128/jb.183.22.6509-6516.2001;
RA Rahman A., Barr K., Rick P.D.;
RT "Identification of the structural gene for the TDP-Fuc4NAc:Lipid II Fuc4NAc
RT transferase involved in synthesis of enterobacterial common antigen in
RT Escherichia coli K-12.";
RL J. Bacteriol. 183:6509-6516(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=1379743; DOI=10.1126/science.1379743;
RA Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
RT "Analysis of the Escherichia coli genome: DNA sequence of the region from
RT 84.5 to 86.5 minutes.";
RL Science 257:771-778(1992).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP SEQUENCE REVISION.
RX PubMed=16397293; DOI=10.1093/nar/gkj405;
RA Riley M., Abe T., Arnaud M.B., Berlyn M.K.B., Blattner F.R.,
RA Chaudhuri R.R., Glasner J.D., Horiuchi T., Keseler I.M., Kosuge T.,
RA Mori H., Perna N.T., Plunkett G. III, Rudd K.E., Serres M.H., Thomas G.H.,
RA Thomson N.R., Wishart D., Wanner B.L.;
RT "Escherichia coli K-12: a cooperatively developed annotation snapshot
RT -- 2005.";
RL Nucleic Acids Res. 34:1-9(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
CC -!- FUNCTION: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA-Fuc4NAc
CC (Lipid III), the third lipid-linked intermediate involved in ECA
CC synthesis. {ECO:0000269|PubMed:11673418}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=beta-D-ManNAcA-(1->4)-alpha-D-GlcNAc-di-trans,octa-cis-
CC undecaprenyl diphosphate + dTDP-4-acetamido-4,6-dideoxy-alpha-D-
CC galactose = alpha-D-FucNAc4-(1->4)-beta-D-ManNAcA-(1->4)-D-GlcNAc-
CC undecaprenyl diphosphate + dTDP + H(+); Xref=Rhea:RHEA:28759,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:58369, ChEBI:CHEBI:61495,
CC ChEBI:CHEBI:61496, ChEBI:CHEBI:68493; EC=2.4.1.325;
CC Evidence={ECO:0000269|PubMed:11673418};
CC -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC antigen biosynthesis.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Peripheral
CC membrane protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 56 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA67593.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AF375882; AAK62972.1; -; Genomic_DNA.
DR EMBL; M87049; AAA67593.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U00096; AAT48215.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77505.1; -; Genomic_DNA.
DR RefSeq; WP_000217234.1; NZ_LN832404.1.
DR RefSeq; YP_026257.1; NC_000913.3.
DR AlphaFoldDB; P56258; -.
DR BioGRID; 4262106; 182.
DR BioGRID; 853430; 3.
DR IntAct; P56258; 3.
DR STRING; 511145.b4481; -.
DR CAZy; GT56; Glycosyltransferase Family 56.
DR jPOST; P56258; -.
DR PaxDb; P56258; -.
DR PRIDE; P56258; -.
DR EnsemblBacteria; AAT48215; AAT48215; b4481.
DR EnsemblBacteria; BAE77505; BAE77505; BAE77505.
DR GeneID; 2847677; -.
DR KEGG; ecj:JW5596; -.
DR KEGG; eco:b4481; -.
DR PATRIC; fig|1411691.4.peg.2913; -.
DR EchoBASE; EB4066; -.
DR eggNOG; COG0554; Bacteria.
DR HOGENOM; CLU_066584_0_0_6; -.
DR OMA; VIVPMGY; -.
DR PhylomeDB; P56258; -.
DR BioCyc; EcoCyc:G7800-MON; -.
DR BioCyc; MetaCyc:G7800-MON; -.
DR UniPathway; UPA00566; -.
DR PRO; PR:P56258; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0102031; F:4-acetamido-4,6-dideoxy-D-galactose transferase activity; IDA:EcoCyc.
DR GO; GO:0008417; F:fucosyltransferase activity; IEA:InterPro.
DR GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IMP:EcoCyc.
DR GO; GO:0036065; P:fucosylation; IEA:InterPro.
DR HAMAP; MF_01002; WecF_RffT; 1.
DR InterPro; IPR009993; WecF.
DR Pfam; PF07429; Glyco_transf_56; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Glycosyltransferase; Membrane;
KW Reference proteome; Transferase.
FT CHAIN 1..359
FT /note="TDP-N-acetylfucosamine:lipid II N-
FT acetylfucosaminyltransferase"
FT /id="PRO_0000216180"
FT CONFLICT 132
FT /note="A -> G (in Ref. 2; AAA67593)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 359 AA; 40640 MW; 092E2813918DC1D1 CRC64;
MTVLIHVLGS DIPHHNRTVL RFFNDALAAT SEHAREFMVV GKDDGLSDSC PALSVQFFPG
KKSLAEAVIA KAKANRQQRF FFHGQFNPTL WLALLSGGIK PSQFFWHIWG ADLYELSSGL
RYKLFYPLRR LAQKRVGCVF ATRGDLSFFA KTHPKVRGEL LFFPTRMDPS LNTMANDRQR
EGKMTILVGN SGDRSNEHIA ALRAVHQQFG DTVKVVVPMG YPPNNEAYIE EVRQAGLELF
SEENLQILSE KLEFDAYLAL LRQCDLGYFI FARQQGIGTL CLLIQAGIPC VLNRENPFWQ
DMTEQHLPVL FTTDDLNEDI VREAQRQLAS VDKNTIAFFS PNYLQGWQRA LAIAAREVA
