ID   WECF_SALG2              Reviewed;         359 AA.
AC   B5RFR1;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   04-NOV-2008, sequence version 1.
DT   03-AUG-2022, entry version 67.
DE   RecName: Full=TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferase {ECO:0000255|HAMAP-Rule:MF_01002};
DE            EC=2.4.1.325 {ECO:0000255|HAMAP-Rule:MF_01002};
DE   AltName: Full=4-alpha-L-fucosyltransferase {ECO:0000255|HAMAP-Rule:MF_01002};
DE   AltName: Full=TDP-Fuc4NAc:lipid II Fuc4NAc transferase {ECO:0000255|HAMAP-Rule:MF_01002};
DE            Short=Fuc4NAc transferase {ECO:0000255|HAMAP-Rule:MF_01002};
GN   Name=wecF {ECO:0000255|HAMAP-Rule:MF_01002};
GN   Synonyms=rffT {ECO:0000255|HAMAP-Rule:MF_01002}; OrderedLocusNames=SG3516;
OS   Salmonella gallinarum (strain 287/91 / NCTC 13346).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=550538;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=287/91 / NCTC 13346;
RX   PubMed=18583645; DOI=10.1101/gr.077404.108;
RA   Thomson N.R., Clayton D.J., Windhorst D., Vernikos G., Davidson S.,
RA   Churcher C., Quail M.A., Stevens M., Jones M.A., Watson M., Barron A.,
RA   Layton A., Pickard D., Kingsley R.A., Bignell A., Clark L., Harris B.,
RA   Ormond D., Abdellah Z., Brooks K., Cherevach I., Chillingworth T.,
RA   Woodward J., Norberczak H., Lord A., Arrowsmith C., Jagels K., Moule S.,
RA   Mungall K., Saunders M., Whitehead S., Chabalgoity J.A., Maskell D.,
RA   Humphreys T., Roberts M., Barrow P.A., Dougan G., Parkhill J.;
RT   "Comparative genome analysis of Salmonella enteritidis PT4 and Salmonella
RT   gallinarum 287/91 provides insights into evolutionary and host adaptation
RT   pathways.";
RL   Genome Res. 18:1624-1637(2008).
CC   -!- FUNCTION: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA-Fuc4NAc
CC       (Lipid III), the third lipid-linked intermediate involved in ECA
CC       synthesis. {ECO:0000255|HAMAP-Rule:MF_01002}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=beta-D-ManNAcA-(1->4)-alpha-D-GlcNAc-di-trans,octa-cis-
CC         undecaprenyl diphosphate + dTDP-4-acetamido-4,6-dideoxy-alpha-D-
CC         galactose = alpha-D-FucNAc4-(1->4)-beta-D-ManNAcA-(1->4)-D-GlcNAc-
CC         undecaprenyl diphosphate + dTDP + H(+); Xref=Rhea:RHEA:28759,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:58369, ChEBI:CHEBI:61495,
CC         ChEBI:CHEBI:61496, ChEBI:CHEBI:68493; EC=2.4.1.325;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01002};
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC       antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01002}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC       Rule:MF_01002}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC       Rule:MF_01002}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 56 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01002}.
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DR   EMBL; AM933173; CAR39305.1; -; Genomic_DNA.
DR   RefSeq; WP_000217198.1; NC_011274.1.
DR   AlphaFoldDB; B5RFR1; -.
DR   CAZy; GT56; Glycosyltransferase Family 56.
DR   EnsemblBacteria; CAR39305; CAR39305; SG3516.
DR   KEGG; seg:SG3516; -.
DR   HOGENOM; CLU_066584_0_0_6; -.
DR   OMA; VIVPMGY; -.
DR   UniPathway; UPA00566; -.
DR   Proteomes; UP000008321; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0102031; F:4-acetamido-4,6-dideoxy-D-galactose transferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008417; F:fucosyltransferase activity; IEA:InterPro.
DR   GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0036065; P:fucosylation; IEA:InterPro.
DR   HAMAP; MF_01002; WecF_RffT; 1.
DR   InterPro; IPR009993; WecF.
DR   Pfam; PF07429; Glyco_transf_56; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Glycosyltransferase; Membrane;
KW   Transferase.
FT   CHAIN           1..359
FT                   /note="TDP-N-acetylfucosamine:lipid II N-
FT                   acetylfucosaminyltransferase"
FT                   /id="PRO_1000134606"
SQ   SEQUENCE   359 AA;  40468 MW;  76DB84DA7A4A6787 CRC64;
     MTVLIHVLGS DIPHHNHTVL RFFNDTLAAT SEHAREFMVA GEDNGFTESC PALSLRFYGS
     KKALAQAVIA KAKANRRQRF FFHGQFNTSL WLALLSGGIK PAQFYWHIWG ADLYEVSNGL
     KFRLFYPLRR IAQGRVGCVF ATRGDLSYFA RQHPNVRGEL LYFPTRMDPS LNAMAKECQR
     AGKLTILVGN SGDRSNQHIA ALRAVYQQFG DTVNVVVPMG YPANNQAYID EVRQAGLALF
     SAENLQILSE KMEFDAYLAL LRQCDLGYFI FARQQGIGTL CLLIQADIPC VLNRDNPFWQ
     DMAEQHLPVL FTTDDLNEQV VREAQRQLAS VDKSGITFFS PNYLQPWHNA LRIAAGEAE