ID   WECG_ECODH              Reviewed;         246 AA.
AC   B1XAG6;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 1.
DT   03-AUG-2022, entry version 70.
DE   RecName: Full=UDP-N-acetyl-D-mannosaminuronic acid transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE            Short=UDP-ManNAcA transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE            EC=2.4.1.180 {ECO:0000255|HAMAP-Rule:MF_01001};
GN   Name=wecG {ECO:0000255|HAMAP-Rule:MF_01001};
GN   Synonyms=rffM {ECO:0000255|HAMAP-Rule:MF_01001};
GN   OrderedLocusNames=ECDH10B_3984;
OS   Escherichia coli (strain K12 / DH10B).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=316385;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / DH10B;
RX   PubMed=18245285; DOI=10.1128/jb.01695-07;
RA   Durfee T., Nelson R., Baldwin S., Plunkett G. III, Burland V., Mau B.,
RA   Petrosino J.F., Qin X., Muzny D.M., Ayele M., Gibbs R.A., Csorgo B.,
RA   Posfai G., Weinstock G.M., Blattner F.R.;
RT   "The complete genome sequence of Escherichia coli DH10B: insights into the
RT   biology of a laboratory workhorse.";
RL   J. Bacteriol. 190:2597-2606(2008).
CC   -!- FUNCTION: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II),
CC       the second lipid-linked intermediate involved in enterobacterial common
CC       antigen (ECA) synthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=N-acetyl-alpha-D-glucosaminyl-di-trans,octa-cis-undecaprenyl
CC         diphosphate + UDP-N-acetyl-alpha-D-mannosaminouronate = beta-D-
CC         ManNAcA-(1->4)-alpha-D-GlcNAc-di-trans,octa-cis-undecaprenyl
CC         diphosphate + H(+) + UDP; Xref=Rhea:RHEA:28366, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:61495, ChEBI:CHEBI:62959,
CC         ChEBI:CHEBI:70731; EC=2.4.1.180; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01001};
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC       antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 26 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01001}.
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DR   EMBL; CP000948; ACB04824.1; -; Genomic_DNA.
DR   RefSeq; WP_001064040.1; NC_010473.1.
DR   AlphaFoldDB; B1XAG6; -.
DR   SMR; B1XAG6; -.
DR   CAZy; GT26; Glycosyltransferase Family 26.
DR   KEGG; ecd:ECDH10B_3984; -.
DR   HOGENOM; CLU_063203_3_2_6; -.
DR   OMA; PWRWRRM; -.
DR   BioCyc; ECOL316385:ECDH10B_RS20275-MON; -.
DR   UniPathway; UPA00566; -.
DR   GO; GO:0047241; F:lipopolysaccharide N-acetylmannosaminouronosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd06533; Glyco_transf_WecG_TagA; 1.
DR   HAMAP; MF_01001; WecG_RffM; 1.
DR   InterPro; IPR023085; UDP-ManNAcA_Trfase_WecG.
DR   InterPro; IPR004629; WecG_TagA_CpsF.
DR   PANTHER; PTHR34136; PTHR34136; 1.
DR   Pfam; PF03808; Glyco_tran_WecG; 1.
DR   TIGRFAMs; TIGR00696; wecG_tagA_cpsF; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Transferase.
FT   CHAIN           1..246
FT                   /note="UDP-N-acetyl-D-mannosaminuronic acid transferase"
FT                   /id="PRO_1000134575"
SQ   SEQUENCE   246 AA;  27928 MW;  0D9435DEA6A69CAC CRC64;
     MNNNTTAPTY TLRGLQLIGW RDMQHALDYL FADGQLKQGT LVAINAEKML TIEDNAEVRE
     LINAAEFKYA DGISVVRSVR KKYPQAQVSR VAGADLWEEL MARAGKEGTP VFLVGGKPEV
     LAQTEAKLRN QWNVNIVGSQ DGYFKPEQRQ ALFERIHASG AQIVTVAMGS PKQEIIMRDC
     RLVHPDALYM GVGGTYDVFT GHVKRAPKIW QTLGLEWLYR LLSQPSRIKR QLRLLRYLRW
     HYTGNL