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WECG_ECOLI
ID   WECG_ECOLI              Reviewed;         246 AA.
AC   P27836; Q2M8A3;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   15-JUL-1998, sequence version 2.
DT   03-AUG-2022, entry version 154.
DE   RecName: Full=UDP-N-acetyl-D-mannosaminuronic acid transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE            Short=UDP-ManNAcA transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE            EC=2.4.1.180 {ECO:0000255|HAMAP-Rule:MF_01001};
GN   Name=wecG {ECO:0000255|HAMAP-Rule:MF_01001};
GN   Synonyms=rffM {ECO:0000255|HAMAP-Rule:MF_01001};
GN   OrderedLocusNames=b3794, JW3770;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=1379743; DOI=10.1126/science.1379743;
RA   Daniels D.L., Plunkett G. III, Burland V.D., Blattner F.R.;
RT   "Analysis of the Escherichia coli genome: DNA sequence of the region from
RT   84.5 to 86.5 minutes.";
RL   Science 257:771-778(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND SEQUENCE REVISION TO
RP   120-121.
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   CATALYTIC ACTIVITY.
RX   PubMed=3275612; DOI=10.1128/jb.170.1.228-233.1988;
RA   Barr K., Ward S., Meier-Dieter U., Mayer H., Rick P.D.;
RT   "Characterization of an Escherichia coli rff mutant defective in transfer
RT   of N-acetylmannosaminuronic acid (ManNAcA) from UDP-ManNAcA to a lipid-
RT   linked intermediate involved in enterobacterial common antigen synthesis.";
RL   J. Bacteriol. 170:228-233(1988).
RN   [5]
RP   FUNCTION.
RX   PubMed=1730666; DOI=10.1016/s0021-9258(18)48347-0;
RA   Meier-Dieter U., Barr K., Starman R., Hatch L., Rick P.D.;
RT   "Nucleotide sequence of the Escherichia coli rfe gene involved in the
RT   synthesis of enterobacterial common antigen. Molecular cloning of the rfe-
RT   rff gene cluster.";
RL   J. Biol. Chem. 267:746-753(1992).
CC   -!- FUNCTION: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II),
CC       the second lipid-linked intermediate involved in enterobacterial common
CC       antigen (ECA) synthesis. {ECO:0000255|HAMAP-Rule:MF_01001,
CC       ECO:0000269|PubMed:1730666}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=N-acetyl-alpha-D-glucosaminyl-di-trans,octa-cis-undecaprenyl
CC         diphosphate + UDP-N-acetyl-alpha-D-mannosaminouronate = beta-D-
CC         ManNAcA-(1->4)-alpha-D-GlcNAc-di-trans,octa-cis-undecaprenyl
CC         diphosphate + H(+) + UDP; Xref=Rhea:RHEA:28366, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:61495, ChEBI:CHEBI:62959,
CC         ChEBI:CHEBI:70731; EC=2.4.1.180; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01001, ECO:0000269|PubMed:3275612};
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC       antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 26 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01001}.
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DR   EMBL; M87049; AAA67594.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC76801.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE77503.1; -; Genomic_DNA.
DR   PIR; G65183; G65183.
DR   RefSeq; NP_418242.1; NC_000913.3.
DR   RefSeq; WP_001064040.1; NZ_SSZK01000025.1.
DR   AlphaFoldDB; P27836; -.
DR   SMR; P27836; -.
DR   BioGRID; 4261340; 190.
DR   BioGRID; 852599; 1.
DR   DIP; DIP-11132N; -.
DR   IntAct; P27836; 15.
DR   STRING; 511145.b3794; -.
DR   CAZy; GT26; Glycosyltransferase Family 26.
DR   jPOST; P27836; -.
DR   PaxDb; P27836; -.
DR   PRIDE; P27836; -.
DR   DNASU; 948301; -.
DR   EnsemblBacteria; AAC76801; AAC76801; b3794.
DR   EnsemblBacteria; BAE77503; BAE77503; BAE77503.
DR   GeneID; 948301; -.
DR   KEGG; ecj:JW3770; -.
DR   KEGG; eco:b3794; -.
DR   PATRIC; fig|1411691.4.peg.2911; -.
DR   EchoBASE; EB1427; -.
DR   eggNOG; COG1922; Bacteria.
DR   HOGENOM; CLU_063203_3_2_6; -.
DR   InParanoid; P27836; -.
DR   OMA; PWRWRRM; -.
DR   PhylomeDB; P27836; -.
DR   BioCyc; EcoCyc:UDPMANACATRANS-MON; -.
DR   BioCyc; MetaCyc:UDPMANACATRANS-MON; -.
DR   UniPathway; UPA00566; -.
DR   PRO; PR:P27836; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016758; F:hexosyltransferase activity; IMP:EcoCyc.
DR   GO; GO:0047241; F:lipopolysaccharide N-acetylmannosaminouronosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IMP:EcoCyc.
DR   CDD; cd06533; Glyco_transf_WecG_TagA; 1.
DR   HAMAP; MF_01001; WecG_RffM; 1.
DR   InterPro; IPR023085; UDP-ManNAcA_Trfase_WecG.
DR   InterPro; IPR004629; WecG_TagA_CpsF.
DR   PANTHER; PTHR34136; PTHR34136; 1.
DR   Pfam; PF03808; Glyco_tran_WecG; 1.
DR   TIGRFAMs; TIGR00696; wecG_tagA_cpsF; 1.
PE   1: Evidence at protein level;
KW   Glycosyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..246
FT                   /note="UDP-N-acetyl-D-mannosaminuronic acid transferase"
FT                   /id="PRO_0000208426"
FT   CONFLICT        120..121
FT                   /note="VL -> AV (in Ref. 1; AAA67594)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   246 AA;  27928 MW;  0D9435DEA6A69CAC CRC64;
     MNNNTTAPTY TLRGLQLIGW RDMQHALDYL FADGQLKQGT LVAINAEKML TIEDNAEVRE
     LINAAEFKYA DGISVVRSVR KKYPQAQVSR VAGADLWEEL MARAGKEGTP VFLVGGKPEV
     LAQTEAKLRN QWNVNIVGSQ DGYFKPEQRQ ALFERIHASG AQIVTVAMGS PKQEIIMRDC
     RLVHPDALYM GVGGTYDVFT GHVKRAPKIW QTLGLEWLYR LLSQPSRIKR QLRLLRYLRW
     HYTGNL
 
 
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