WECG_ECOSE
ID WECG_ECOSE Reviewed; 246 AA.
AC B6I4D2;
DT 14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 1.
DT 25-MAY-2022, entry version 63.
DE RecName: Full=UDP-N-acetyl-D-mannosaminuronic acid transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE Short=UDP-ManNAcA transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE EC=2.4.1.180 {ECO:0000255|HAMAP-Rule:MF_01001};
GN Name=wecG {ECO:0000255|HAMAP-Rule:MF_01001};
GN Synonyms=rffM {ECO:0000255|HAMAP-Rule:MF_01001};
GN OrderedLocusNames=ECSE_4078;
OS Escherichia coli (strain SE11).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=409438;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SE11;
RX PubMed=18931093; DOI=10.1093/dnares/dsn026;
RA Oshima K., Toh H., Ogura Y., Sasamoto H., Morita H., Park S.-H., Ooka T.,
RA Iyoda S., Taylor T.D., Hayashi T., Itoh K., Hattori M.;
RT "Complete genome sequence and comparative analysis of the wild-type
RT commensal Escherichia coli strain SE11 isolated from a healthy adult.";
RL DNA Res. 15:375-386(2008).
CC -!- FUNCTION: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II),
CC the second lipid-linked intermediate involved in enterobacterial common
CC antigen (ECA) synthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N-acetyl-alpha-D-glucosaminyl-di-trans,octa-cis-undecaprenyl
CC diphosphate + UDP-N-acetyl-alpha-D-mannosaminouronate = beta-D-
CC ManNAcA-(1->4)-alpha-D-GlcNAc-di-trans,octa-cis-undecaprenyl
CC diphosphate + H(+) + UDP; Xref=Rhea:RHEA:28366, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:61495, ChEBI:CHEBI:62959,
CC ChEBI:CHEBI:70731; EC=2.4.1.180; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01001};
CC -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 26 family.
CC {ECO:0000255|HAMAP-Rule:MF_01001}.
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DR EMBL; AP009240; BAG79602.1; -; Genomic_DNA.
DR RefSeq; WP_001064038.1; NC_011415.1.
DR AlphaFoldDB; B6I4D2; -.
DR SMR; B6I4D2; -.
DR CAZy; GT26; Glycosyltransferase Family 26.
DR EnsemblBacteria; BAG79602; BAG79602; ECSE_4078.
DR GeneID; 66672302; -.
DR KEGG; ecy:ECSE_4078; -.
DR HOGENOM; CLU_063203_3_2_6; -.
DR OMA; PWRWRRM; -.
DR UniPathway; UPA00566; -.
DR Proteomes; UP000008199; Chromosome.
DR GO; GO:0047241; F:lipopolysaccharide N-acetylmannosaminouronosyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd06533; Glyco_transf_WecG_TagA; 1.
DR HAMAP; MF_01001; WecG_RffM; 1.
DR InterPro; IPR023085; UDP-ManNAcA_Trfase_WecG.
DR InterPro; IPR004629; WecG_TagA_CpsF.
DR PANTHER; PTHR34136; PTHR34136; 1.
DR Pfam; PF03808; Glyco_tran_WecG; 1.
DR TIGRFAMs; TIGR00696; wecG_tagA_cpsF; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase; Transferase.
FT CHAIN 1..246
FT /note="UDP-N-acetyl-D-mannosaminuronic acid transferase"
FT /id="PRO_1000134577"
SQ SEQUENCE 246 AA; 27962 MW; CE98CA2DA6A69535 CRC64;
MNNNTTAPTY TLRGLQLIGW RDMQHALDYL FADGQLKQGT LVAINAEKML TIEDNAEVRE
LINAAEFKYA DGISVVRSVR KKYPQAQVSR VAGADLWEEL MARAGKEGTP VFLVGGKPEV
LAQTEAKLRN QWNVNIVGSQ DGYFKPEQRQ ALFERIHASG AQIVTVAMGS PKQEIFMRDC
RLVHPDALYM GVGGTYDVFT GHVKRAPKIW QTLGLEWLYR LLSQPSRIKR QLRLLRYLRW
HYTGNL