WECG_PECAS
ID WECG_PECAS Reviewed; 249 AA.
AC Q6CZF2;
DT 13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 16-AUG-2004, sequence version 1.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=UDP-N-acetyl-D-mannosaminuronic acid transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE Short=UDP-ManNAcA transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE EC=2.4.1.180 {ECO:0000255|HAMAP-Rule:MF_01001};
GN Name=wecG {ECO:0000255|HAMAP-Rule:MF_01001};
GN Synonyms=rffM {ECO:0000255|HAMAP-Rule:MF_01001}; OrderedLocusNames=ECA4200;
OS Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672) (Erwinia
OS carotovora subsp. atroseptica).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Pectobacteriaceae; Pectobacterium.
OX NCBI_TaxID=218491;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SCRI 1043 / ATCC BAA-672;
RX PubMed=15263089; DOI=10.1073/pnas.0402424101;
RA Bell K.S., Sebaihia M., Pritchard L., Holden M.T.G., Hyman L.J.,
RA Holeva M.C., Thomson N.R., Bentley S.D., Churcher L.J.C., Mungall K.,
RA Atkin R., Bason N., Brooks K., Chillingworth T., Clark K., Doggett J.,
RA Fraser A., Hance Z., Hauser H., Jagels K., Moule S., Norbertczak H.,
RA Ormond D., Price C., Quail M.A., Sanders M., Walker D., Whitehead S.,
RA Salmond G.P.C., Birch P.R.J., Parkhill J., Toth I.K.;
RT "Genome sequence of the enterobacterial phytopathogen Erwinia carotovora
RT subsp. atroseptica and characterization of virulence factors.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:11105-11110(2004).
CC -!- FUNCTION: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II),
CC the second lipid-linked intermediate involved in enterobacterial common
CC antigen (ECA) synthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N-acetyl-alpha-D-glucosaminyl-di-trans,octa-cis-undecaprenyl
CC diphosphate + UDP-N-acetyl-alpha-D-mannosaminouronate = beta-D-
CC ManNAcA-(1->4)-alpha-D-GlcNAc-di-trans,octa-cis-undecaprenyl
CC diphosphate + H(+) + UDP; Xref=Rhea:RHEA:28366, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:61495, ChEBI:CHEBI:62959,
CC ChEBI:CHEBI:70731; EC=2.4.1.180; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01001};
CC -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 26 family.
CC {ECO:0000255|HAMAP-Rule:MF_01001}.
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DR EMBL; BX950851; CAG77097.1; -; Genomic_DNA.
DR RefSeq; WP_011095671.1; NC_004547.2.
DR AlphaFoldDB; Q6CZF2; -.
DR SMR; Q6CZF2; -.
DR STRING; 218491.ECA4200; -.
DR CAZy; GT26; Glycosyltransferase Family 26.
DR EnsemblBacteria; CAG77097; CAG77097; ECA4200.
DR GeneID; 57210866; -.
DR KEGG; eca:ECA4200; -.
DR PATRIC; fig|218491.5.peg.4276; -.
DR eggNOG; COG1922; Bacteria.
DR HOGENOM; CLU_063203_3_2_6; -.
DR OMA; PWRWRRM; -.
DR OrthoDB; 1759731at2; -.
DR UniPathway; UPA00566; -.
DR Proteomes; UP000007966; Chromosome.
DR GO; GO:0047241; F:lipopolysaccharide N-acetylmannosaminouronosyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd06533; Glyco_transf_WecG_TagA; 1.
DR HAMAP; MF_01001; WecG_RffM; 1.
DR InterPro; IPR023085; UDP-ManNAcA_Trfase_WecG.
DR InterPro; IPR004629; WecG_TagA_CpsF.
DR PANTHER; PTHR34136; PTHR34136; 1.
DR Pfam; PF03808; Glyco_tran_WecG; 1.
DR TIGRFAMs; TIGR00696; wecG_tagA_cpsF; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase; Reference proteome; Transferase.
FT CHAIN 1..249
FT /note="UDP-N-acetyl-D-mannosaminuronic acid transferase"
FT /id="PRO_0000208429"
SQ SEQUENCE 249 AA; 28282 MW; 16DC27A780A8A95A CRC64;
MTALKTTETI PLYTIRGLPI HGFRNMGTFV DYLFAGERVE TGTLVAINAE KVLTAEKEVA
LRTLLDRAEY KYADGISIVR SIRRKYPQAD VTRIAGADLW EALMERAGKQ ETPVFLVGGK
PDVLAQTEAK LRAQWDVNIV GSQDGYFTPE QRDALFERIR ASGAQIVTVA MGSPRQEILM
RDCRHHYPDA LYMGIGGTYD VFTGHVKRAP LVWQNLGLEW LYRLLSQPSR IFRQLRLLKY
VAYHYSGRL