ID   WECG_PROMH              Reviewed;         245 AA.
AC   B4F1W8;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   23-SEP-2008, sequence version 1.
DT   25-MAY-2022, entry version 78.
DE   RecName: Full=UDP-N-acetyl-D-mannosaminuronic acid transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE            Short=UDP-ManNAcA transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE            EC=2.4.1.180 {ECO:0000255|HAMAP-Rule:MF_01001};
GN   Name=wecG {ECO:0000255|HAMAP-Rule:MF_01001};
GN   Synonyms=rffM {ECO:0000255|HAMAP-Rule:MF_01001}; OrderedLocusNames=PMI3327;
OS   Proteus mirabilis (strain HI4320).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Proteus.
OX   NCBI_TaxID=529507;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HI4320;
RX   PubMed=18375554; DOI=10.1128/jb.01981-07;
RA   Pearson M.M., Sebaihia M., Churcher C., Quail M.A., Seshasayee A.S.,
RA   Luscombe N.M., Abdellah Z., Arrosmith C., Atkin B., Chillingworth T.,
RA   Hauser H., Jagels K., Moule S., Mungall K., Norbertczak H.,
RA   Rabbinowitsch E., Walker D., Whithead S., Thomson N.R., Rather P.N.,
RA   Parkhill J., Mobley H.L.T.;
RT   "Complete genome sequence of uropathogenic Proteus mirabilis, a master of
RT   both adherence and motility.";
RL   J. Bacteriol. 190:4027-4037(2008).
CC   -!- FUNCTION: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II),
CC       the second lipid-linked intermediate involved in enterobacterial common
CC       antigen (ECA) synthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=N-acetyl-alpha-D-glucosaminyl-di-trans,octa-cis-undecaprenyl
CC         diphosphate + UDP-N-acetyl-alpha-D-mannosaminouronate = beta-D-
CC         ManNAcA-(1->4)-alpha-D-GlcNAc-di-trans,octa-cis-undecaprenyl
CC         diphosphate + H(+) + UDP; Xref=Rhea:RHEA:28366, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:61495, ChEBI:CHEBI:62959,
CC         ChEBI:CHEBI:70731; EC=2.4.1.180; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01001};
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC       antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 26 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01001}.
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DR   EMBL; AM942759; CAR46526.1; -; Genomic_DNA.
DR   RefSeq; WP_004246339.1; NC_010554.1.
DR   AlphaFoldDB; B4F1W8; -.
DR   SMR; B4F1W8; -.
DR   STRING; 529507.PMI3327; -.
DR   CAZy; GT26; Glycosyltransferase Family 26.
DR   EnsemblBacteria; CAR46526; CAR46526; PMI3327.
DR   GeneID; 6801694; -.
DR   KEGG; pmr:PMI3327; -.
DR   eggNOG; COG1922; Bacteria.
DR   HOGENOM; CLU_063203_3_2_6; -.
DR   OMA; PWRWRRM; -.
DR   UniPathway; UPA00566; -.
DR   Proteomes; UP000008319; Chromosome.
DR   GO; GO:0047241; F:lipopolysaccharide N-acetylmannosaminouronosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd06533; Glyco_transf_WecG_TagA; 1.
DR   HAMAP; MF_01001; WecG_RffM; 1.
DR   InterPro; IPR028082; Peripla_BP_I.
DR   InterPro; IPR023085; UDP-ManNAcA_Trfase_WecG.
DR   InterPro; IPR004629; WecG_TagA_CpsF.
DR   PANTHER; PTHR34136; PTHR34136; 1.
DR   Pfam; PF03808; Glyco_tran_WecG; 1.
DR   SUPFAM; SSF53822; SSF53822; 1.
DR   TIGRFAMs; TIGR00696; wecG_tagA_cpsF; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..245
FT                   /note="UDP-N-acetyl-D-mannosaminuronic acid transferase"
FT                   /id="PRO_1000134581"
SQ   SEQUENCE   245 AA;  27583 MW;  CBF7DCD20E18EF0C CRC64;
     MDTNSIPKYS IRGHNIWGFK DMAHFLDYLY QGAQIKSGSL IAINAEKILT AETDKGLNQL
     LDEADYLYAD GISVVRGIRR KYPDAQVSRI AGADLWEALM ERAGKEGTPV FLVGGKPQVL
     AQTESKLKAQ WNVNIVGSQD GYFTPQDRDA LFERIKASGA KIVTIAMGSP KQELFIRACR
     QVHPDALYMG VGGTYDVFTG HVKRAPKAWQ NLGLEWLYRL LSQPSRIKRQ FKLLKFVGYY
     YSNKL