ID   WECG_SALPA              Reviewed;         246 AA.
AC   Q5PKK7;
DT   13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT   04-JAN-2005, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=UDP-N-acetyl-D-mannosaminuronic acid transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE            Short=UDP-ManNAcA transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE            EC=2.4.1.180 {ECO:0000255|HAMAP-Rule:MF_01001};
GN   Name=wecG {ECO:0000255|HAMAP-Rule:MF_01001};
GN   Synonyms=rffM {ECO:0000255|HAMAP-Rule:MF_01001}; OrderedLocusNames=SPA3769;
OS   Salmonella paratyphi A (strain ATCC 9150 / SARB42).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=295319;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 9150 / SARB42;
RX   PubMed=15531882; DOI=10.1038/ng1470;
RA   McClelland M., Sanderson K.E., Clifton S.W., Latreille P., Porwollik S.,
RA   Sabo A., Meyer R., Bieri T., Ozersky P., McLellan M., Harkins C.R.,
RA   Wang C., Nguyen C., Berghoff A., Elliott G., Kohlberg S., Strong C., Du F.,
RA   Carter J., Kremizki C., Layman D., Leonard S., Sun H., Fulton L., Nash W.,
RA   Miner T., Minx P., Delehaunty K., Fronick C., Magrini V., Nhan M.,
RA   Warren W., Florea L., Spieth J., Wilson R.K.;
RT   "Comparison of genome degradation in Paratyphi A and Typhi, human-
RT   restricted serovars of Salmonella enterica that cause typhoid.";
RL   Nat. Genet. 36:1268-1274(2004).
CC   -!- FUNCTION: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II),
CC       the second lipid-linked intermediate involved in enterobacterial common
CC       antigen (ECA) synthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=N-acetyl-alpha-D-glucosaminyl-di-trans,octa-cis-undecaprenyl
CC         diphosphate + UDP-N-acetyl-alpha-D-mannosaminouronate = beta-D-
CC         ManNAcA-(1->4)-alpha-D-GlcNAc-di-trans,octa-cis-undecaprenyl
CC         diphosphate + H(+) + UDP; Xref=Rhea:RHEA:28366, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:61495, ChEBI:CHEBI:62959,
CC         ChEBI:CHEBI:70731; EC=2.4.1.180; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01001};
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC       antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 26 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01001}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; CP000026; AAV79551.1; -; Genomic_DNA.
DR   RefSeq; WP_000183626.1; NC_006511.1.
DR   AlphaFoldDB; Q5PKK7; -.
DR   SMR; Q5PKK7; -.
DR   CAZy; GT26; Glycosyltransferase Family 26.
DR   EnsemblBacteria; AAV79551; AAV79551; SPA3769.
DR   KEGG; spt:SPA3769; -.
DR   HOGENOM; CLU_063203_3_2_6; -.
DR   OMA; NMNGTDF; -.
DR   UniPathway; UPA00566; -.
DR   Proteomes; UP000008185; Chromosome.
DR   GO; GO:0047241; F:lipopolysaccharide N-acetylmannosaminouronosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd06533; Glyco_transf_WecG_TagA; 1.
DR   HAMAP; MF_01001; WecG_RffM; 1.
DR   InterPro; IPR023085; UDP-ManNAcA_Trfase_WecG.
DR   InterPro; IPR004629; WecG_TagA_CpsF.
DR   PANTHER; PTHR34136; PTHR34136; 1.
DR   Pfam; PF03808; Glyco_tran_WecG; 1.
DR   TIGRFAMs; TIGR00696; wecG_tagA_cpsF; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Transferase.
FT   CHAIN           1..246
FT                   /note="UDP-N-acetyl-D-mannosaminuronic acid transferase"
FT                   /id="PRO_0000208432"
SQ   SEQUENCE   246 AA;  27557 MW;  4273ACFBDD3F390F CRC64;
     MTNNAAAPLY SLRGLPLIGW RDMSHALNYL FADGQLKQGT LVAINAEKLL TAEDNPEVRA
     LIGAAEFKYA DGISVVRSIR KKFPQAQVSR VAGADLWEAL MARAGKEGTP VFLVGGKPEV
     LAQTEAKLRT QWNVNIVGSQ DGYFTPEQRQ ALFARIHASG AKIVTVAMGS PKQELLMRDC
     REVHPHALYM GVGGTYDVFT GHVKRAPKIW QNLGLEWLYR LLSQPKRITR QMRLLRYLRW
     HYTGDL