WECG_SERP5
ID WECG_SERP5 Reviewed; 246 AA.
AC A8G842;
DT 26-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT 13-NOV-2007, sequence version 1.
DT 25-MAY-2022, entry version 68.
DE RecName: Full=UDP-N-acetyl-D-mannosaminuronic acid transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE Short=UDP-ManNAcA transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE EC=2.4.1.180 {ECO:0000255|HAMAP-Rule:MF_01001};
GN Name=wecG {ECO:0000255|HAMAP-Rule:MF_01001};
GN Synonyms=rffM {ECO:0000255|HAMAP-Rule:MF_01001};
GN OrderedLocusNames=Spro_0172;
OS Serratia proteamaculans (strain 568).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Yersiniaceae; Serratia.
OX NCBI_TaxID=399741;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=568;
RG US DOE Joint Genome Institute;
RA Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E.,
RA Tice H., Pitluck S., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J.,
RA Larimer F., Land M., Hauser L., Kyrpides N., Kim E., Taghavi S., Newman L.,
RA Vangronsveld J., van der Lelie D., Richardson P.;
RT "Complete sequence of chromosome of Serratia proteamaculans 568.";
RL Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II),
CC the second lipid-linked intermediate involved in enterobacterial common
CC antigen (ECA) synthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=N-acetyl-alpha-D-glucosaminyl-di-trans,octa-cis-undecaprenyl
CC diphosphate + UDP-N-acetyl-alpha-D-mannosaminouronate = beta-D-
CC ManNAcA-(1->4)-alpha-D-GlcNAc-di-trans,octa-cis-undecaprenyl
CC diphosphate + H(+) + UDP; Xref=Rhea:RHEA:28366, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:61495, ChEBI:CHEBI:62959,
CC ChEBI:CHEBI:70731; EC=2.4.1.180; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_01001};
CC -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 26 family.
CC {ECO:0000255|HAMAP-Rule:MF_01001}.
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DR EMBL; CP000826; ABV39282.1; -; Genomic_DNA.
DR RefSeq; WP_012004643.1; NC_009832.1.
DR AlphaFoldDB; A8G842; -.
DR SMR; A8G842; -.
DR STRING; 399741.Spro_0172; -.
DR CAZy; GT26; Glycosyltransferase Family 26.
DR EnsemblBacteria; ABV39282; ABV39282; Spro_0172.
DR KEGG; spe:Spro_0172; -.
DR eggNOG; COG1922; Bacteria.
DR HOGENOM; CLU_063203_3_2_6; -.
DR OMA; PWRWRRM; -.
DR OrthoDB; 1759731at2; -.
DR UniPathway; UPA00566; -.
DR GO; GO:0047241; F:lipopolysaccharide N-acetylmannosaminouronosyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR CDD; cd06533; Glyco_transf_WecG_TagA; 1.
DR HAMAP; MF_01001; WecG_RffM; 1.
DR InterPro; IPR023085; UDP-ManNAcA_Trfase_WecG.
DR InterPro; IPR004629; WecG_TagA_CpsF.
DR PANTHER; PTHR34136; PTHR34136; 1.
DR Pfam; PF03808; Glyco_tran_WecG; 1.
DR TIGRFAMs; TIGR00696; wecG_tagA_cpsF; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase; Transferase.
FT CHAIN 1..246
FT /note="UDP-N-acetyl-D-mannosaminuronic acid transferase"
FT /id="PRO_0000318861"
SQ SEQUENCE 246 AA; 27960 MW; B79D7D09824D9CE2 CRC64;
MEAKISVPKY ELRGFSLWGF RDMAQCMDFL FDGGRVKRGT LVAMNAEKIL TAEQDTELHA
LLDEAEYKYA DGISMVRSIR RKYPGADVSR VAGADLWEAL MQRAGREGTP VFLIGGKPSV
LAETEQKLRS QWNVNLVGSQ DGYFKPEQRE PLFERIRASG AAIVTVAMGS PKQEILMRDC
RKVHPQALYM GVGGTYDVFT GHVKRAPKVW QNLGLEWLYR LLSQPSRLGR QLRLLKFVSY
YYRGKM