ID   WECG_YERPB              Reviewed;         246 AA.
AC   B2K060;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   10-JUN-2008, sequence version 1.
DT   03-AUG-2022, entry version 63.
DE   RecName: Full=UDP-N-acetyl-D-mannosaminuronic acid transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE            Short=UDP-ManNAcA transferase {ECO:0000255|HAMAP-Rule:MF_01001};
DE            EC=2.4.1.180 {ECO:0000255|HAMAP-Rule:MF_01001};
GN   Name=wecG {ECO:0000255|HAMAP-Rule:MF_01001};
GN   Synonyms=rffM {ECO:0000255|HAMAP-Rule:MF_01001};
GN   OrderedLocusNames=YPTS_0191;
OS   Yersinia pseudotuberculosis serotype IB (strain PB1/+).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=502801;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PB1/+;
RA   Copeland A., Lucas S., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Munk A.C., Brettin T., Detter J.C.,
RA   Han C., Tapia R., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Challacombe J.F., Green L., Lindler L.E., Nikolich M.P., Richardson P.;
RT   "Complete sequence of Yersinia pseudotuberculosis PB1/+.";
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II),
CC       the second lipid-linked intermediate involved in enterobacterial common
CC       antigen (ECA) synthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=N-acetyl-alpha-D-glucosaminyl-di-trans,octa-cis-undecaprenyl
CC         diphosphate + UDP-N-acetyl-alpha-D-mannosaminouronate = beta-D-
CC         ManNAcA-(1->4)-alpha-D-GlcNAc-di-trans,octa-cis-undecaprenyl
CC         diphosphate + H(+) + UDP; Xref=Rhea:RHEA:28366, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:61495, ChEBI:CHEBI:62959,
CC         ChEBI:CHEBI:70731; EC=2.4.1.180; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01001};
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; enterobacterial common
CC       antigen biosynthesis. {ECO:0000255|HAMAP-Rule:MF_01001}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 26 family.
CC       {ECO:0000255|HAMAP-Rule:MF_01001}.
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DR   EMBL; CP001048; ACC87188.1; -; Genomic_DNA.
DR   RefSeq; WP_011191504.1; NZ_CP009780.1.
DR   AlphaFoldDB; B2K060; -.
DR   SMR; B2K060; -.
DR   CAZy; GT26; Glycosyltransferase Family 26.
DR   GeneID; 66843420; -.
DR   KEGG; ypb:YPTS_0191; -.
DR   PATRIC; fig|502801.10.peg.3868; -.
DR   OMA; PWRWRRM; -.
DR   BioCyc; YPSE502801:YPTS_RS00990-MON; -.
DR   UniPathway; UPA00566; -.
DR   GO; GO:0047241; F:lipopolysaccharide N-acetylmannosaminouronosyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0009246; P:enterobacterial common antigen biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd06533; Glyco_transf_WecG_TagA; 1.
DR   HAMAP; MF_01001; WecG_RffM; 1.
DR   InterPro; IPR023085; UDP-ManNAcA_Trfase_WecG.
DR   InterPro; IPR004629; WecG_TagA_CpsF.
DR   PANTHER; PTHR34136; PTHR34136; 1.
DR   Pfam; PF03808; Glyco_tran_WecG; 1.
DR   TIGRFAMs; TIGR00696; wecG_tagA_cpsF; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Transferase.
FT   CHAIN           1..246
FT                   /note="UDP-N-acetyl-D-mannosaminuronic acid transferase"
FT                   /id="PRO_1000134591"
SQ   SEQUENCE   246 AA;  27646 MW;  220670C5D2222F26 CRC64;
     MEPNTVIPKY NVRGFEIWGF RDMAQVLDHL LGSGPVKTGT LVAMNAEKLL KAEDDTALCE
     LIKNAEYLYA DGISMVRAIR RKYPQAELSR VAGADLWEAL MQRAGQQGTP VFLVGGKPDV
     LAETEAKLRA QWNVNLVGSQ DGYFTPEQRE ALFARIAASG AAIVTVAMGS PKQEIFMRDC
     RKFYPDALYM GVGGTYDVFT GHVKRAPKIW QNMGLEWLYR LLAQPSRIRR QLKLLKFVGY
     YYSGRL