WEE1_ORYSJ
ID WEE1_ORYSJ Reviewed; 520 AA.
AC Q6Z829; A0A0P0VED2; Q0E458;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 120.
DE RecName: Full=Wee1-like protein kinase;
DE EC=2.7.10.2;
GN Name=WEE1; OrderedLocusNames=Os02g0135300, LOC_Os02g04240;
GN ORFNames=P0585B01.27;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [4]
RP GENE FAMILY.
RX PubMed=17443292; DOI=10.1007/s11103-007-9154-y;
RA Guo J., Song J., Wang F., Zhang X.S.;
RT "Genome-wide identification and expression analysis of rice cell cycle
RT genes.";
RL Plant Mol. Biol. 64:349-360(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-
CC [protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-
CC COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858,
CC ChEBI:CHEBI:82620, ChEBI:CHEBI:456216; EC=2.7.10.2;
CC Evidence={ECO:0000255|PROSITE-ProRule:PRU10027};
CC -!- SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein
CC kinase family. WEE1 subfamily. {ECO:0000255|PROSITE-ProRule:PRU00159}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF07730.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AP004799; BAD10095.1; -; Genomic_DNA.
DR EMBL; AP008208; BAF07730.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014958; BAS76855.1; -; Genomic_DNA.
DR RefSeq; XP_015626457.1; XM_015770971.1.
DR AlphaFoldDB; Q6Z829; -.
DR SMR; Q6Z829; -.
DR STRING; 4530.OS02T0135300-00; -.
DR PaxDb; Q6Z829; -.
DR PRIDE; Q6Z829; -.
DR EnsemblPlants; Os02t0135300-00; Os02t0135300-00; Os02g0135300.
DR GeneID; 4328224; -.
DR Gramene; Os02t0135300-00; Os02t0135300-00; Os02g0135300.
DR KEGG; osa:4328224; -.
DR eggNOG; KOG0601; Eukaryota.
DR HOGENOM; CLU_000288_25_0_1; -.
DR InParanoid; Q6Z829; -.
DR OMA; WFENEKL; -.
DR OrthoDB; 1063695at2759; -.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR Genevisible; Q6Z829; OS.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004715; F:non-membrane spanning protein tyrosine kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0004672; F:protein kinase activity; IBA:GO_Central.
DR GO; GO:0004713; F:protein tyrosine kinase activity; IBA:GO_Central.
DR GO; GO:1902750; P:negative regulation of cell cycle G2/M phase transition; IEA:EnsemblPlants.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR017441; Protein_kinase_ATP_BS.
DR InterPro; IPR008271; Ser/Thr_kinase_AS.
DR Pfam; PF00069; Pkinase; 1.
DR SMART; SM00220; S_TKc; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00107; PROTEIN_KINASE_ATP; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
DR PROSITE; PS00108; PROTEIN_KINASE_ST; 1.
PE 3: Inferred from homology;
KW ATP-binding; Kinase; Magnesium; Metal-binding; Nucleotide-binding;
KW Phosphoprotein; Reference proteome; Transferase; Tyrosine-protein kinase.
FT CHAIN 1..520
FT /note="Wee1-like protein kinase"
FT /id="PRO_0000295673"
FT DOMAIN 267..510
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT REGION 1..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 61..103
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 17..38
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 82..97
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 387
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159,
FT ECO:0000255|PROSITE-ProRule:PRU10027"
FT BINDING 273..281
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 296
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT BINDING 392
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
FT BINDING 404
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000250"
SQ SEQUENCE 520 AA; 58391 MW; 934F492877B99F9F CRC64;
MLRTKTPRPR GGKSRRATAA AGKERERERE REREGRSPSG ELSLQLEHVS LFSFLADAPR
EGAAAARTPF TPFEELLEGS CDPDPTPPPP LPPLQPQATP MDADEVVEEK DSGILSQDFF
CTPDYITPDA PQLGSGFDAN KENIPCPNSP EKSVCRSKRY KRDCSPKGLG SNDIFDSQWI
APVQFEGLDD SEEEQLKESS SHKRGSYVSQ SAVALRCRVM PPPCIRNPYL NTDHQIDDNV
FGGRQCKSSG FSPSVDGDGL SRYRTDFHEI EQIGRGNFSV VFKVLKRIDG CLYAVKRSIR
QLHNDRERRQ AVKEVQALAA LGCHENIVGY FTSWFENKQL FIQMELCDRC LSMDRNQPLK
CGEALELLYQ ICKGLDFIHE RGIAHLDVKP DNIYVRNGVY KLGDFGCATL IDRSLAIEDG
DSRYMPPEML NDKYEHLDKV DIFSLGAAIY ELIRGTQLPD SGPQFTSLRE GKIALLPGCP
MQFQSLIKSM MDPDPVRRPS AKEVLRHPIF DKLHKAPAKK
