WER_ARATH
ID WER_ARATH Reviewed; 203 AA.
AC Q9SEI0; Q9ZTD2;
DT 01-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Transcription factor WER;
DE AltName: Full=Myb-related protein 66;
DE Short=AtMYB66;
DE AltName: Full=Protein WEREWOLF;
GN Name=WER; Synonyms=MYB66; OrderedLocusNames=At5g14750; ORFNames=T9L3.50;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=10589676; DOI=10.1016/s0092-8674(00)81536-6;
RA Lee M.M., Schiefelbein J.;
RT "WEREWOLF, a MYB-related protein in Arabidopsis, is a position-dependent
RT regulator of epidermal cell patterning.";
RL Cell 99:473-483(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L.-J., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: a genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 76-203.
RC STRAIN=cv. Columbia;
RX PubMed=9839469; DOI=10.1046/j.1365-313x.1998.00278.x;
RA Kranz H.D., Denekamp M., Greco R., Jin H.-L., Leyva A., Meissner R.C.,
RA Petroni K., Urzainqui A., Bevan M., Martin C., Smeekens S., Tonelli C.,
RA Paz-Ares J., Weisshaar B.;
RT "Towards functional characterisation of the members of the R2R3-MYB gene
RT family from Arabidopsis thaliana.";
RL Plant J. 16:263-276(1998).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [8]
RP FUNCTION.
RX PubMed=11585796; DOI=10.1242/dev.128.19.3697;
RA Lin Y., Schiefelbein J.;
RT "Embryonic control of epidermal cell patterning in the root and hypocotyl
RT of Arabidopsis.";
RL Development 128:3697-3705(2001).
RN [9]
RP INDUCTION.
RX PubMed=11910008; DOI=10.1105/tpc.010434;
RA Lee M.M., Schiefelbein J.;
RT "Cell pattern in the Arabidopsis root epidermis determined by lateral
RT inhibition with feedback.";
RL Plant Cell 14:611-618(2002).
RN [10]
RP FUNCTION, INTERACTION WITH GL3 AND BHLH2, AND TISSUE SPECIFICITY.
RX PubMed=14627722; DOI=10.1242/dev.00880;
RA Bernhardt C., Lee M.M., Gonzalez A., Zhang F., Lloyd A.M., Schiefelbein J.;
RT "The bHLH genes GLABRA3 (GL3) and ENHANCER OF GLABRA3 (EGL3) specify
RT epidermal cell fate in the Arabidopsis root.";
RL Development 130:6431-6439(2003).
RN [11]
RP FUNCTION, AND INTERACTION WITH BHLH2 AND BHLH12.
RX PubMed=15361138; DOI=10.1111/j.1365-313x.2004.02183.x;
RA Zimmermann I.M., Heim M.A., Weisshaar B., Uhrig J.F.;
RT "Comprehensive identification of Arabidopsis thaliana MYB transcription
RT factors interacting with R/B-like BHLH proteins.";
RL Plant J. 40:22-34(2004).
RN [12]
RP INDUCTION BY HDA18.
RX PubMed=16176989; DOI=10.1073/pnas.0503143102;
RA Xu C.-R., Liu C., Wang Y.-L., Li L.-C., Chen W.-Q., Xu Z.-H., Bai S.-N.;
RT "Histone acetylation affects expression of cellular patterning genes in the
RT Arabidopsis root epidermis.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:14469-14474(2005).
RN [13]
RP FUNCTION.
RX PubMed=15795220; DOI=10.1093/pcp/pci096;
RA Koshino-Kimura Y., Wada T., Tachibana T., Tsugeki R., Ishiguro S.,
RA Okada K.;
RT "Regulation of CAPRICE transcription by MYB proteins for root epidermis
RT differentiation in Arabidopsis.";
RL Plant Cell Physiol. 46:817-826(2005).
RN [14]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=16207757; DOI=10.1242/dev.02055;
RA Ryu K.H., Kang Y.H., Park Y.-H., Hwang I., Schiefelbein J., Lee M.M.;
RT "The WEREWOLF MYB protein directly regulates CAPRICE transcription during
RT cell fate specification in the Arabidopsis root epidermis.";
RL Development 132:4765-4775(2005).
RN [15]
RP GENE FAMILY.
RX PubMed=16463103; DOI=10.1007/s11103-005-2910-y;
RA Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X.,
RA Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H.,
RA Qu L.-J.;
RT "The MYB transcription factor superfamily of Arabidopsis: expression
RT analysis and phylogenetic comparison with the rice MYB family.";
RL Plant Mol. Biol. 60:107-124(2006).
CC -!- FUNCTION: Transcription activator, when associated with
CC BHLH2/EGL3/MYC146 or BHLH12/MYC1. Involved in epidermal cell fate
CC specification in roots and hypocotyl. Together with GL3 or BHLH2,
CC promotes the formation of non-hair developing cells (atrichoblasts) et
CC the N position in root epidermis. Regulates stomata spatial
CC distribution in hypocotyls. Binds to the WER-binding sites (WBS)
CC promoter regions and activates the transcription of target genes such
CC as GL2 and of CPC. {ECO:0000269|PubMed:10589676,
CC ECO:0000269|PubMed:11585796, ECO:0000269|PubMed:14627722,
CC ECO:0000269|PubMed:15361138, ECO:0000269|PubMed:15795220,
CC ECO:0000269|PubMed:16207757}.
CC -!- SUBUNIT: Interacts with GL3, BHLH2/EGL3/MYC146 and BHLH12/MYC1.
CC {ECO:0000269|PubMed:14627722, ECO:0000269|PubMed:15361138}.
CC -!- INTERACTION:
CC Q9SEI0; Q9CAD0: BHLH2; NbExp=4; IntAct=EBI-533373, EBI-533398;
CC Q9SEI0; Q9FN69: GL3; NbExp=3; IntAct=EBI-533373, EBI-533348;
CC Q9SEI0; Q9LJI5: VHA-d1; NbExp=3; IntAct=EBI-533373, EBI-25520180;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00625,
CC ECO:0000269|PubMed:16207757}.
CC -!- TISSUE SPECIFICITY: Restricted to roots and hypocotyl. Specifically
CC expressed in root non-hair developing cells (atrichoblasts) at the N
CC position. Also present in lateral root cap cells. In hypocotyls,
CC expressed within files of epidermal cells located outside a single
CC cortical cell equivalent to roots N cells (at protein level).
CC {ECO:0000269|PubMed:10589676, ECO:0000269|PubMed:14627722,
CC ECO:0000269|PubMed:16207757}.
CC -!- INDUCTION: Transcriptional activation correlates with reduced histone
CC acetylation on H3 and H4 mediated by HDA18 in N cells. Repressed by CPC
CC in hair cells (H position). {ECO:0000269|PubMed:11910008,
CC ECO:0000269|PubMed:16176989}.
CC -!- MISCELLANEOUS: This protein is called 'Werewolf' because plants lacking
CC WER exhibit hairy roots.
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DR EMBL; AF126399; AAF18939.1; -; Genomic_DNA.
DR EMBL; AY519623; AAS10093.1; -; mRNA.
DR EMBL; AL391149; CAC01874.1; -; Genomic_DNA.
DR EMBL; CP002688; AED92073.1; -; Genomic_DNA.
DR EMBL; BT026346; ABH04453.1; -; mRNA.
DR EMBL; AF062900; AAC83622.1; -; mRNA.
DR PIR; T51420; T51420.
DR PIR; T51672; T51672.
DR RefSeq; NP_196979.1; NM_121479.3.
DR PDB; 6KKS; X-ray; 2.15 A; A=12-130.
DR PDBsum; 6KKS; -.
DR AlphaFoldDB; Q9SEI0; -.
DR SMR; Q9SEI0; -.
DR BioGRID; 16604; 11.
DR IntAct; Q9SEI0; 7.
DR STRING; 3702.AT5G14750.1; -.
DR PaxDb; Q9SEI0; -.
DR PRIDE; Q9SEI0; -.
DR EnsemblPlants; AT5G14750.1; AT5G14750.1; AT5G14750.
DR GeneID; 831327; -.
DR Gramene; AT5G14750.1; AT5G14750.1; AT5G14750.
DR KEGG; ath:AT5G14750; -.
DR Araport; AT5G14750; -.
DR TAIR; locus:2185470; AT5G14750.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_028567_25_8_1; -.
DR InParanoid; Q9SEI0; -.
DR OMA; NQWNTRI; -.
DR OrthoDB; 1499244at2759; -.
DR PhylomeDB; Q9SEI0; -.
DR PRO; PR:Q9SEI0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9SEI0; baseline and differential.
DR Genevisible; Q9SEI0; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IPI:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0045165; P:cell fate commitment; IMP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:TAIR.
DR GO; GO:0048765; P:root hair cell differentiation; IMP:TAIR.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Developmental protein; DNA-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation.
FT CHAIN 1..203
FT /note="Transcription factor WER"
FT /id="PRO_0000285273"
FT DOMAIN 13..65
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 66..120
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 41..65
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 93..116
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT CONFLICT 134
FT /note="I -> M (in Ref. 6; AAC83622)"
FT /evidence="ECO:0000305"
FT HELIX 23..36
FT /evidence="ECO:0007829|PDB:6KKS"
FT HELIX 43..47
FT /evidence="ECO:0007829|PDB:6KKS"
FT HELIX 54..63
FT /evidence="ECO:0007829|PDB:6KKS"
FT HELIX 76..89
FT /evidence="ECO:0007829|PDB:6KKS"
FT HELIX 93..97
FT /evidence="ECO:0007829|PDB:6KKS"
FT HELIX 105..115
FT /evidence="ECO:0007829|PDB:6KKS"
SQ SEQUENCE 203 AA; 23569 MW; EFAB8A342F0FB66F CRC64;
MRKKVSSSGD EGNNEYKKGL WTVEEDKILM DYVKAHGKGH WNRIAKKTGL KRCGKSCRLR
WMNYLSPNVK RGNFTEQEED LIIRLHKLLG NRWSLIAKRV PGRTDNQVKN YWNTHLSKKL
GIKDQKTKQS NGDIVYQINL PNPTETSEET KISNIVDNNN ILGDEIQEDH QGSNYLSSLW
VHEDEFELST LTNMMDFIDG HCF
