WETA_EMENI
ID WETA_EMENI Reviewed; 555 AA.
AC P22022; C8VKV9; Q5BBZ3;
DT 01-AUG-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1996, sequence version 2.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Developmental regulatory protein wetA {ECO:0000305};
GN Name=wetA {ECO:0000303|PubMed:1986246}; ORFNames=AN1937;
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=1986246; DOI=10.1128/mcb.11.1.55-62.1991;
RA Marshall M.A., Timberlake W.E.;
RT "Aspergillus nidulans wetA activates spore-specific gene expression.";
RL Mol. Cell. Biol. 11:55-62(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
RN [4]
RP DISRUPTION PHENOTYPE, AND INDUCTION.
RX PubMed=2823119; DOI=10.1128/mcb.7.9.3113-3118.1987;
RA Boylan M.T., Mirabito P.M., Willett C.E., Zimmerman C.R., Timberlake W.E.;
RT "Isolation and physical characterization of three essential conidiation
RT genes from Aspergillus nidulans.";
RL Mol. Cell. Biol. 7:3113-3118(1987).
RN [5]
RP FUNCTION, AND INDUCTION.
RX PubMed=2655931; DOI=10.1016/0092-8674(89)90800-3;
RA Mirabito P.M., Adams T.H., Timberlake W.E.;
RT "Interactions of three sequentially expressed genes control temporal and
RT spatial specificity in Aspergillus development.";
RL Cell 57:859-868(1989).
RN [6]
RP FUNCTION.
RX PubMed=2180753; DOI=10.1016/0012-1606(90)90215-5;
RA Sewall T.C., Mims C.W., Timberlake W.E.;
RT "Conidium differentiation in Aspergillus nidulans wild-type and wet-white
RT (wetA) mutant strains.";
RL Dev. Biol. 138:499-508(1990).
RN [7]
RP FUNCTION.
RX PubMed=7704830; DOI=10.1139/m94-164;
RA Sewall T.C.;
RT "Cellular effects of misscheduled brlA, abaA, and wetA expression in
RT Aspergillus nidulans.";
RL Can. J. Microbiol. 40:1035-1042(1994).
RN [8]
RP INDUCTION.
RX PubMed=24587098; DOI=10.1371/journal.pone.0089883;
RA Park H.S., Nam T.Y., Han K.H., Kim S.C., Yu J.H.;
RT "VelC positively controls sexual development in Aspergillus nidulans.";
RL PLoS ONE 9:E89883-E89883(2014).
CC -!- FUNCTION: BrlA, abaA and wetA are pivotal regulators of conidiophore
CC development and conidium maturation. They act individually and together
CC to regulate their own expression and that of numerous other
CC sporulation-specific genes (PubMed:2655931). Responsible for activating
CC a set of genes whose products make up the final two conidial wall
CC layers or direct their assembly and, through this activity, is
CC responsible for acquisition of spore dormancy (PubMed:1986246,
CC PubMed:2180753, PubMed:7704830). {ECO:0000269|PubMed:1986246,
CC ECO:0000269|PubMed:2180753, ECO:0000269|PubMed:2655931,
CC ECO:0000269|PubMed:7704830}.
CC -!- DEVELOPMENTAL STAGE: The wetA gene is activated only during
CC conidiophore development, and its mRNA accumulates preferentially in
CC mature conidia.
CC -!- INDUCTION: Expression is induced during conidiation, after conidia had
CC begun to form (PubMed:2823119). Positively regulated by abaA
CC (PubMed:2655931). Negatively regulated by velC (PubMed:24587098).
CC {ECO:0000269|PubMed:24587098, ECO:0000269|PubMed:2655931,
CC ECO:0000269|PubMed:2823119}.
CC -!- DOMAIN: Has an acidic N-terminus (AA 1-52) followed by a Ser-, Thr-,
CC Pro-rich domain (AA 125-233) and a basic C-terminus (AA 461-555).
CC -!- DISRUPTION PHENOTYPE: Prevents the formation of normal conidiophores
CC (PubMed:2823119). {ECO:0000269|PubMed:2823119}.
CC -!- SIMILARITY: Belongs to the wetA family. {ECO:0000305}.
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DR EMBL; M60528; AAA33330.1; -; Genomic_DNA.
DR EMBL; AACD01000029; EAA65102.1; -; Genomic_DNA.
DR EMBL; BN001307; CBF85858.1; -; Genomic_DNA.
DR PIR; A39665; RGASWA.
DR RefSeq; XP_659541.1; XM_654449.1.
DR AlphaFoldDB; P22022; -.
DR STRING; 162425.CADANIAP00008597; -.
DR EnsemblFungi; CBF85858; CBF85858; ANIA_01937.
DR EnsemblFungi; EAA65102; EAA65102; AN1937.2.
DR GeneID; 2875691; -.
DR KEGG; ani:AN1937.2; -.
DR VEuPathDB; FungiDB:AN1937; -.
DR eggNOG; ENOG502S8IT; Eukaryota.
DR HOGENOM; CLU_030750_0_0_1; -.
DR InParanoid; P22022; -.
DR OMA; MAYQEAW; -.
DR OrthoDB; 638649at2759; -.
DR Proteomes; UP000000560; Chromosome VII.
DR Proteomes; UP000005890; Unassembled WGS sequence.
DR GO; GO:0042243; P:asexual spore wall assembly; IMP:AspGD.
DR GO; GO:0048315; P:conidium formation; IMP:AspGD.
DR GO; GO:0046148; P:pigment biosynthetic process; IMP:AspGD.
DR GO; GO:0075307; P:positive regulation of conidium formation; IMP:AspGD.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:AspGD.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:AspGD.
DR InterPro; IPR040112; WetA.
DR PANTHER; PTHR22934:SF23; PTHR22934:SF23; 1.
PE 2: Evidence at transcript level;
KW Activator; Conidiation; Reference proteome; Sporulation; Transcription;
KW Transcription regulation.
FT CHAIN 1..555
FT /note="Developmental regulatory protein wetA"
FT /id="PRO_0000065961"
FT REGION 113..171
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 250..310
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 419..488
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 508..531
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 144..158
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 419..439
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 462..488
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 517..531
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 555 AA; 60276 MW; 4C9F51708D61400E CRC64;
MFAHPFDHSF NDLFNQYVNM DTSSTDANKD VSFPSEFDQL FPLDSFSTDC GDQSPVISTV
QHNSQPAQDW GKDLWSLSQN TGCSTNQDSF SFQDSTQPST ALDLSIGLEA DATGHSQASV
PRSTPSTPPA TPGPKVKGGL FTPKTIRHHR ESNDRRGLLR KQSFSPGLMR SSQLQKGSCR
MAYPEAWAQR LQNFTIRSSD ECLPLSPPPS DILVQQENVK HTPVQMRNAA EGFQGSTELP
QQIDSGYITQ SPAIPMPSPS ANALAGQQQR YLSQTGTSAL TPSPPSARDV FSSPHSSDPQ
SMPSWHSESL NTPAFQYTPE LSDHQTWWSP MPSEVAQRHA SYQQMIASPA PQRPVQAAAN
HGDFLQGGLM IQLDPTQFDI SSSFPSSTIP TTANNHDNLA YNVEAHAPQK YVDASSFNTQ
AVPHPSRSPS ISPKADTSPR HGSANRDGMA MKNAPRRPHG RKLSGQSTST PKPVKTPNSL
STSPRGGKSV TVSFVNFTAN DRQKILTGVA PSGSSKTKAR REQEARDRRR KLSEAALQAV
RKAGGDVEAL EAVLC
