WF15B_MOUSE
ID WF15B_MOUSE Reviewed; 80 AA.
AC Q9JHY4; A2A5M6; Q8BVC0;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=WAP four-disulfide core domain protein 15B;
DE AltName: Full=Elafin-like protein I;
DE AltName: Full=Single WAP motif protein 1;
DE Flags: Precursor;
GN Name=Wfdc15b; Synonyms=Swam1, Wfdc15;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090 {ECO:0000312|EMBL:AAF86471.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, TISSUE SPECIFICITY, AND
RP INDUCTION.
RC STRAIN=129/SvJ {ECO:0000312|EMBL:AAF86471.1};
RX PubMed=12574366; DOI=10.4049/jimmunol.170.4.1973;
RA Hagiwara K., Kikuchi T., Endo Y., Huqun X., Usui K., Takahashi M.,
RA Shibata N., Kusakabe T., Xin H., Hoshi S., Miki M., Inooka N., Tokue Y.,
RA Nukiwa T.;
RT "Mouse SWAM1 and SWAM2 are antibacterial proteins composed of a single whey
RT acidic protein motif.";
RL J. Immunol. 170:1973-1979(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Epididymis, and Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION.
RX PubMed=15950183; DOI=10.1016/j.bbrc.2005.05.125;
RA Clauss A., Lilja H., Lundwall A.;
RT "The evolution of a genetic locus encoding small serine proteinase
RT inhibitors.";
RL Biochem. Biophys. Res. Commun. 333:383-389(2005).
CC -!- FUNCTION: Antibacterial protein which inhibits the growth of E.coli and
CC S.aureus. {ECO:0000269|PubMed:12574366}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Constitutively expressed in kidney and epididymis.
CC {ECO:0000269|PubMed:12574366}.
CC -!- INDUCTION: By intranasal administration of S.pneumoniae.
CC {ECO:0000269|PubMed:12574366}.
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DR EMBL; AF276974; AAF86471.1; -; mRNA.
DR EMBL; AF482009; AAL90747.1; -; Genomic_DNA.
DR EMBL; AK078998; BAC37499.1; -; mRNA.
DR EMBL; AK085392; BAC39438.1; -; mRNA.
DR EMBL; AL591512; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC061041; AAH61041.1; -; mRNA.
DR CCDS; CCDS38320.1; -.
DR RefSeq; NP_001039019.1; NM_001045554.1.
DR RefSeq; NP_619626.1; NM_138685.2.
DR RefSeq; XP_006499020.1; XM_006498957.3.
DR AlphaFoldDB; Q9JHY4; -.
DR SMR; Q9JHY4; -.
DR STRING; 10090.ENSMUSP00000105001; -.
DR PaxDb; Q9JHY4; -.
DR PRIDE; Q9JHY4; -.
DR ProteomicsDB; 299764; -.
DR DNASU; 192201; -.
DR Ensembl; ENSMUST00000018355; ENSMUSP00000018355; ENSMUSG00000018211.
DR Ensembl; ENSMUST00000109376; ENSMUSP00000105001; ENSMUSG00000018211.
DR Ensembl; ENSMUST00000164567; ENSMUSP00000132507; ENSMUSG00000018211.
DR GeneID; 192201; -.
DR KEGG; mmu:192201; -.
DR UCSC; uc008ntz.1; mouse.
DR CTD; 192201; -.
DR MGI; MGI:2445041; Wfdc15b.
DR VEuPathDB; HostDB:ENSMUSG00000018211; -.
DR eggNOG; ENOG502TEXR; Eukaryota.
DR GeneTree; ENSGT00390000002529; -.
DR HOGENOM; CLU_200089_0_0_1; -.
DR InParanoid; Q9JHY4; -.
DR OMA; PEFFLEC; -.
DR OrthoDB; 1571744at2759; -.
DR PhylomeDB; Q9JHY4; -.
DR TreeFam; TF339673; -.
DR BioGRID-ORCS; 192201; 1 hit in 69 CRISPR screens.
DR ChiTaRS; Wfdc15b; mouse.
DR PRO; PR:Q9JHY4; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9JHY4; protein.
DR Bgee; ENSMUSG00000018211; Expressed in right kidney and 61 other tissues.
DR Genevisible; Q9JHY4; MM.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IBA:GO_Central.
DR GO; GO:0019731; P:antibacterial humoral response; IBA:GO_Central.
DR GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR Gene3D; 4.10.75.10; -; 1.
DR InterPro; IPR036645; Elafin-like_sf.
DR InterPro; IPR008197; WAP_dom.
DR Pfam; PF00095; WAP; 1.
DR PRINTS; PR00003; 4DISULPHCORE.
DR SMART; SM00217; WAP; 1.
DR SUPFAM; SSF57256; SSF57256; 1.
DR PROSITE; PS51390; WAP; 1.
PE 2: Evidence at transcript level;
KW Antibiotic; Antimicrobial; Disulfide bond; Reference proteome; Secreted;
KW Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..80
FT /note="WAP four-disulfide core domain protein 15B"
FT /id="PRO_0000041394"
FT DOMAIN 29..76
FT /note="WAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 36..64
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 43..68
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 51..63
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 57..72
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
SQ SEQUENCE 80 AA; 9237 MW; 9135647ED91F63ED CRC64;
MKLLGLSLLA VTILLCCNMA RPEIKKKNVF SKPGYCPEYR VPCPFVLIPK CRRDKGCKDA
LKCCFFYCQM RCVDPWESPE
