WFD12_HUMAN
ID WFD12_HUMAN Reviewed; 111 AA.
AC Q8WWY7; Q5H980; Q9BR31;
DT 19-OCT-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=WAP four-disulfide core domain protein 12;
DE AltName: Full=Putative protease inhibitor WAP12;
DE AltName: Full=Whey acidic protein 2;
DE Flags: Precursor;
GN Name=WFDC12; Synonyms=C20orf122, WAP2; ORFNames=UNQ544/PRO844;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=11779191; DOI=10.1006/bbrc.2001.6224;
RA Lundwall A., Clauss A.;
RT "Identification of a novel protease inhibitor gene that is highly expressed
RT in the prostate.";
RL Biochem. Biophys. Res. Commun. 290:452-456(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11780052; DOI=10.1038/414865a;
RA Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R.,
RA Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L.,
RA Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P.,
RA Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., Buck D., Burrill W.D.,
RA Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G.,
RA Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E.,
RA Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D.,
RA Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P.,
RA Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E.,
RA Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J.,
RA Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D.,
RA Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S.,
RA Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D.,
RA Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A.,
RA Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T.,
RA Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I.,
RA Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M.,
RA Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D.,
RA Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M.,
RA Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A.,
RA Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L.,
RA Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L.,
RA Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 20.";
RL Nature 414:865-871(2001).
CC -!- FUNCTION: Antibacterial protein. Putative acid-stable proteinase
CC inhibitor.
CC -!- INTERACTION:
CC Q8WWY7; Q12797-6: ASPH; NbExp=3; IntAct=EBI-11958577, EBI-12092171;
CC Q8WWY7; O43765: SGTA; NbExp=3; IntAct=EBI-11958577, EBI-347996;
CC Q8WWY7; Q96EQ0: SGTB; NbExp=3; IntAct=EBI-11958577, EBI-744081;
CC Q8WWY7; Q92504: SLC39A7; NbExp=3; IntAct=EBI-11958577, EBI-1051105;
CC Q8WWY7; Q9UMX0: UBQLN1; NbExp=3; IntAct=EBI-11958577, EBI-741480;
CC Q8WWY7; Q9UHD9: UBQLN2; NbExp=6; IntAct=EBI-11958577, EBI-947187;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Highly expressed in prostate, skin, lung and
CC esophagus. Weakly expressed in skeletal muscle, epididymis, kidney,
CC trachea, salivary gland, testis and seminal vesicle.
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DR EMBL; AY037803; AAK68848.1; -; mRNA.
DR EMBL; AY358678; AAQ89041.1; -; mRNA.
DR EMBL; Z93016; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS13343.1; -.
DR RefSeq; NP_543145.1; NM_080869.1.
DR AlphaFoldDB; Q8WWY7; -.
DR SMR; Q8WWY7; -.
DR BioGRID; 126126; 7.
DR IntAct; Q8WWY7; 6.
DR STRING; 9606.ENSP00000361871; -.
DR MEROPS; I17.003; -.
DR iPTMnet; Q8WWY7; -.
DR PhosphoSitePlus; Q8WWY7; -.
DR BioMuta; WFDC12; -.
DR DMDM; 24212610; -.
DR MassIVE; Q8WWY7; -.
DR PaxDb; Q8WWY7; -.
DR PeptideAtlas; Q8WWY7; -.
DR PRIDE; Q8WWY7; -.
DR ProteomicsDB; 74959; -.
DR Antibodypedia; 53770; 60 antibodies from 16 providers.
DR DNASU; 128488; -.
DR Ensembl; ENST00000372785.3; ENSP00000361871.3; ENSG00000168703.5.
DR GeneID; 128488; -.
DR KEGG; hsa:128488; -.
DR MANE-Select; ENST00000372785.3; ENSP00000361871.3; NM_080869.2; NP_543145.1.
DR UCSC; uc002xnf.2; human.
DR CTD; 128488; -.
DR GeneCards; WFDC12; -.
DR HGNC; HGNC:16115; WFDC12.
DR HPA; ENSG00000168703; Tissue enriched (skin).
DR MIM; 609872; gene.
DR neXtProt; NX_Q8WWY7; -.
DR OpenTargets; ENSG00000168703; -.
DR PharmGKB; PA25663; -.
DR VEuPathDB; HostDB:ENSG00000168703; -.
DR eggNOG; ENOG502TDXW; Eukaryota.
DR GeneTree; ENSGT00390000012286; -.
DR HOGENOM; CLU_172659_0_0_1; -.
DR InParanoid; Q8WWY7; -.
DR OMA; IRKCCYL; -.
DR OrthoDB; 1560605at2759; -.
DR PhylomeDB; Q8WWY7; -.
DR TreeFam; TF338375; -.
DR PathwayCommons; Q8WWY7; -.
DR SignaLink; Q8WWY7; -.
DR BioGRID-ORCS; 128488; 8 hits in 1060 CRISPR screens.
DR GenomeRNAi; 128488; -.
DR Pharos; Q8WWY7; Tbio.
DR PRO; PR:Q8WWY7; -.
DR Proteomes; UP000005640; Chromosome 20.
DR RNAct; Q8WWY7; protein.
DR Bgee; ENSG00000168703; Expressed in penis and 80 other tissues.
DR Genevisible; Q8WWY7; HS.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IBA:GO_Central.
DR GO; GO:0019731; P:antibacterial humoral response; IBA:GO_Central.
DR GO; GO:0042742; P:defense response to bacterium; ISS:UniProtKB.
DR GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR Gene3D; 4.10.75.10; -; 1.
DR InterPro; IPR036645; Elafin-like_sf.
DR InterPro; IPR008197; WAP_dom.
DR InterPro; IPR029718; WFDC12.
DR PANTHER; PTHR19441:SF42; PTHR19441:SF42; 1.
DR Pfam; PF00095; WAP; 1.
DR PRINTS; PR00003; 4DISULPHCORE.
DR SMART; SM00217; WAP; 1.
DR SUPFAM; SSF57256; SSF57256; 1.
DR PROSITE; PS51390; WAP; 1.
PE 1: Evidence at protein level;
KW Antibiotic; Antimicrobial; Disulfide bond; Protease inhibitor;
KW Reference proteome; Secreted; Serine protease inhibitor; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000255"
FT CHAIN 24..111
FT /note="WAP four-disulfide core domain protein 12"
FT /id="PRO_0000041390"
FT DOMAIN 27..74
FT /note="WAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT REGION 80..111
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 34..62
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 41..66
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 49..61
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 55..70
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
SQ SEQUENCE 111 AA; 12050 MW; 2DFB39043F1A0997 CRC64;
MGSSSFLVLM VSLVLVTLVA VEGVKEGIEK AGVCPADNVR CFKSDPPQCH TDQDCLGERK
CCYLHCGFKC VIPVKELEEG GNKDEDVSRP YPEPGWEAKC PGSSSTRCPQ K
