WFD12_MOUSE
ID WFD12_MOUSE Reviewed; 85 AA.
AC Q9JHY3;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 126.
DE RecName: Full=WAP four-disulfide core domain protein 12;
DE AltName: Full=Elafin-like protein II;
DE AltName: Full=Single WAP motif protein 2;
DE AltName: Full=Whey acidic protein 2;
DE Flags: Precursor;
GN Name=Wfdc12 {ECO:0000312|MGI:MGI:2183434};
GN Synonyms=Swam2 {ECO:0000312|MGI:MGI:2183434};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAF86472.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=129/SvJ {ECO:0000312|EMBL:AAF86472.1};
RX PubMed=12574366; DOI=10.4049/jimmunol.170.4.1973;
RA Hagiwara K., Kikuchi T., Endo Y., Huqun X., Usui K., Takahashi M.,
RA Shibata N., Kusakabe T., Xin H., Hoshi S., Miki M., Inooka N., Tokue Y.,
RA Nukiwa T.;
RT "Mouse SWAM1 and SWAM2 are antibacterial proteins composed of a single whey
RT acidic protein motif.";
RL J. Immunol. 170:1973-1979(2003).
RN [2] {ECO:0000312|EMBL:AAL90748.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Salivary gland {ECO:0000312|EMBL:AAH30939.1};
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Antibacterial protein which inhibits the growth of E.coli and
CC S.aureus. Putative acid-stable proteinase inhibitor.
CC {ECO:0000250|UniProtKB:Q8WWY7, ECO:0000269|PubMed:12574366}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Constitutively expressed in tongue.
CC {ECO:0000269|PubMed:12574366}.
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DR EMBL; AF276975; AAF86472.1; -; mRNA.
DR EMBL; AF482010; AAL90748.1; -; Genomic_DNA.
DR EMBL; BC030939; AAH30939.1; -; mRNA.
DR CCDS; CCDS38318.1; -.
DR RefSeq; NP_619625.1; NM_138684.2.
DR AlphaFoldDB; Q9JHY3; -.
DR SMR; Q9JHY3; -.
DR STRING; 10090.ENSMUSP00000050836; -.
DR MEROPS; I17.003; -.
DR iPTMnet; Q9JHY3; -.
DR PhosphoSitePlus; Q9JHY3; -.
DR PaxDb; Q9JHY3; -.
DR PRIDE; Q9JHY3; -.
DR ProteomicsDB; 299670; -.
DR Antibodypedia; 53770; 60 antibodies from 16 providers.
DR DNASU; 192200; -.
DR Ensembl; ENSMUST00000051272; ENSMUSP00000050836; ENSMUSG00000042845.
DR GeneID; 192200; -.
DR KEGG; mmu:192200; -.
DR UCSC; uc008ntx.1; mouse.
DR CTD; 128488; -.
DR MGI; MGI:2183434; Wfdc12.
DR VEuPathDB; HostDB:ENSMUSG00000042845; -.
DR GeneTree; ENSGT00390000012286; -.
DR HOGENOM; CLU_172659_0_0_1; -.
DR InParanoid; Q9JHY3; -.
DR OMA; IRKCCYL; -.
DR OrthoDB; 1560605at2759; -.
DR PhylomeDB; Q9JHY3; -.
DR BioGRID-ORCS; 192200; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Wfdc12; mouse.
DR PRO; PR:Q9JHY3; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9JHY3; protein.
DR Bgee; ENSMUSG00000042845; Expressed in parotid gland and 46 other tissues.
DR ExpressionAtlas; Q9JHY3; baseline and differential.
DR Genevisible; Q9JHY3; MM.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IBA:GO_Central.
DR GO; GO:0019731; P:antibacterial humoral response; IBA:GO_Central.
DR GO; GO:0042742; P:defense response to bacterium; IDA:UniProtKB.
DR GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR Gene3D; 4.10.75.10; -; 1.
DR InterPro; IPR036645; Elafin-like_sf.
DR InterPro; IPR008197; WAP_dom.
DR InterPro; IPR029718; WFDC12.
DR PANTHER; PTHR19441:SF42; PTHR19441:SF42; 1.
DR Pfam; PF00095; WAP; 1.
DR SUPFAM; SSF57256; SSF57256; 1.
DR PROSITE; PS51390; WAP; 1.
PE 2: Evidence at transcript level;
KW Antibiotic; Antimicrobial; Disulfide bond; Protease inhibitor;
KW Reference proteome; Secreted; Serine protease inhibitor; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..85
FT /note="WAP four-disulfide core domain protein 12"
FT /evidence="ECO:0000255"
FT /id="PRO_0000041391"
FT DOMAIN 25..72
FT /note="WAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 32..60
FT /evidence="ECO:0000250|UniProtKB:P19957,
FT ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 39..64
FT /evidence="ECO:0000250|UniProtKB:P19957,
FT ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 47..59
FT /evidence="ECO:0000250|UniProtKB:P19957,
FT ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 53..68
FT /evidence="ECO:0000250|UniProtKB:P19957,
FT ECO:0000255|PROSITE-ProRule:PRU00722"
SQ SEQUENCE 85 AA; 9559 MW; 653546C95EF54AD7 CRC64;
MWPNSILVLM TLLISSTLVT GGGVKGEEKR VCPPDYVRCI RQDDPQCYSD NDCGDQEICC
FWQCGFKCVL PVKDNSEEQI PQSKV
