WFDC1_HUMAN
ID WFDC1_HUMAN Reviewed; 220 AA.
AC Q9HC57; D3DUL7; Q8NC27; Q9HAU1;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 02-AUG-2002, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=WAP four-disulfide core domain protein 1;
DE AltName: Full=Prostate stromal protein ps20;
DE AltName: Full=ps20 growth inhibitor;
DE Flags: Precursor;
GN Name=WFDC1; Synonyms=PS20;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT ARG-217.
RC TISSUE=Prostate;
RX PubMed=10967136; DOI=10.1007/s003350010135;
RA Larsen M., Ressler S.J., Gerdes M.J., Lu B., Byron M., Lawrence J.B.,
RA Rowley D.R.;
RT "The WFDC1 gene encoding ps20 localizes to 16q24, a region of LOH in
RT multiple cancers.";
RL Mamm. Genome 11:767-773(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Uterus;
RA Ong C.K., Ng C.Y., Lim K.B., Chan T.W.M.G., Huynh H.;
RT "Molecular cloning and characterization of the human ps20 protein in human
RT uterus.";
RL Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Placenta;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT ARG-217.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon, Kidney, and Stomach;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP VARIANT [LARGE SCALE ANALYSIS] MET-138.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal cancers.";
RL Science 314:268-274(2006).
CC -!- FUNCTION: Has growth inhibitory activity. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG15263.1; Type=Frameshift; Evidence={ECO:0000305};
CC -!- WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and
CC Haematology;
CC URL="http://atlasgeneticsoncology.org/Genes/WFDC1ID424.html";
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DR EMBL; AF169631; AAG16647.1; -; mRNA.
DR EMBL; AF302109; AAG15263.1; ALT_FRAME; mRNA.
DR EMBL; AK075061; BAC11377.1; -; mRNA.
DR EMBL; CH471114; EAW95486.1; -; Genomic_DNA.
DR EMBL; CH471114; EAW95487.1; -; Genomic_DNA.
DR EMBL; BC029159; AAH29159.1; -; mRNA.
DR CCDS; CCDS10946.1; -.
DR RefSeq; NP_001269395.1; NM_001282466.1.
DR RefSeq; NP_001269396.1; NM_001282467.1.
DR RefSeq; NP_067020.2; NM_021197.3.
DR AlphaFoldDB; Q9HC57; -.
DR SMR; Q9HC57; -.
DR BioGRID; 121803; 146.
DR IntAct; Q9HC57; 5.
DR STRING; 9606.ENSP00000219454; -.
DR iPTMnet; Q9HC57; -.
DR PhosphoSitePlus; Q9HC57; -.
DR BioMuta; WFDC1; -.
DR DMDM; 22096244; -.
DR MassIVE; Q9HC57; -.
DR PaxDb; Q9HC57; -.
DR PeptideAtlas; Q9HC57; -.
DR PRIDE; Q9HC57; -.
DR ProteomicsDB; 81641; -.
DR Antibodypedia; 30582; 180 antibodies from 29 providers.
DR DNASU; 58189; -.
DR Ensembl; ENST00000219454.10; ENSP00000219454.5; ENSG00000103175.11.
DR Ensembl; ENST00000568638.1; ENSP00000456920.1; ENSG00000103175.11.
DR GeneID; 58189; -.
DR KEGG; hsa:58189; -.
DR MANE-Select; ENST00000219454.10; ENSP00000219454.5; NM_021197.4; NP_067020.2.
DR UCSC; uc002fhv.5; human.
DR CTD; 58189; -.
DR DisGeNET; 58189; -.
DR GeneCards; WFDC1; -.
DR HGNC; HGNC:15466; WFDC1.
DR HPA; ENSG00000103175; Tissue enhanced (placenta, seminal vesicle).
DR MIM; 605322; gene.
DR neXtProt; NX_Q9HC57; -.
DR OpenTargets; ENSG00000103175; -.
DR PharmGKB; PA37963; -.
DR VEuPathDB; HostDB:ENSG00000103175; -.
DR eggNOG; ENOG502QWDC; Eukaryota.
DR GeneTree; ENSGT00390000014299; -.
DR HOGENOM; CLU_108761_0_0_1; -.
DR InParanoid; Q9HC57; -.
DR OMA; DGHNLRH; -.
DR OrthoDB; 1614363at2759; -.
DR PhylomeDB; Q9HC57; -.
DR TreeFam; TF328768; -.
DR PathwayCommons; Q9HC57; -.
DR SignaLink; Q9HC57; -.
DR BioGRID-ORCS; 58189; 9 hits in 1064 CRISPR screens.
DR ChiTaRS; WFDC1; human.
DR GeneWiki; WFDC1; -.
DR GenomeRNAi; 58189; -.
DR Pharos; Q9HC57; Tbio.
DR PRO; PR:Q9HC57; -.
DR Proteomes; UP000005640; Chromosome 16.
DR RNAct; Q9HC57; protein.
DR Bgee; ENSG00000103175; Expressed in saphenous vein and 165 other tissues.
DR ExpressionAtlas; Q9HC57; baseline and differential.
DR Genevisible; Q9HC57; HS.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0030308; P:negative regulation of cell growth; NAS:UniProtKB.
DR GO; GO:0050728; P:negative regulation of inflammatory response; IEA:Ensembl.
DR GO; GO:0061045; P:negative regulation of wound healing; IEA:Ensembl.
DR GO; GO:0001558; P:regulation of cell growth; IBA:GO_Central.
DR Gene3D; 4.10.75.10; -; 1.
DR InterPro; IPR036645; Elafin-like_sf.
DR InterPro; IPR008197; WAP_dom.
DR InterPro; IPR042357; WFDC1.
DR PANTHER; PTHR14308; PTHR14308; 1.
DR Pfam; PF00095; WAP; 1.
DR SMART; SM00217; WAP; 1.
DR SUPFAM; SSF57256; SSF57256; 1.
DR PROSITE; PS51390; WAP; 1.
PE 2: Evidence at transcript level;
KW Disulfide bond; Protease inhibitor; Reference proteome; Secreted;
KW Serine protease inhibitor; Signal.
FT SIGNAL 1..31
FT /evidence="ECO:0000255"
FT CHAIN 32..220
FT /note="WAP four-disulfide core domain protein 1"
FT /id="PRO_0000041365"
FT DOMAIN 59..108
FT /note="WAP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT REGION 46..70
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 199..220
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 66..96
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 78..100
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 83..95
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 89..104
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT VARIANT 138
FT /note="V -> M (in a breast cancer sample; somatic mutation;
FT dbSNP:rs11643870)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_036489"
FT VARIANT 196
FT /note="L -> V (in dbSNP:rs35504166)"
FT /id="VAR_052948"
FT VARIANT 217
FT /note="K -> R (in dbSNP:rs12933084)"
FT /evidence="ECO:0000269|PubMed:10967136, ECO:0000269|Ref.4"
FT /id="VAR_052949"
FT CONFLICT 33
FT /note="N -> D (in Ref. 3; BAC11377)"
FT /evidence="ECO:0000305"
FT CONFLICT 79
FT /note="Q -> R (in Ref. 3; BAC11377)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 220 AA; 23977 MW; 8098EB9379F88CBD CRC64;
MPLTGVGPGS CRRQIIRALC LLLLLLHAGS AKNIWKRALP ARLAEKSRAE EAGAPGGPRQ
PRADRCPPPP RTLPPGACQA ARCQADSECP RHRRCCYNGC AYACLEAVPP PPVLDWLVQP
KPRWLGGNGW LLDGPEEVLQ AEACSTTEDG AEPLLCPSGY ECHILSPGDV AEGIPNRGQC
VKQRRQADGR ILRHKLYKEY PEGDSKNVAE PGRGQQKHFQ
