WFDC2_RAT
ID WFDC2_RAT Reviewed; 168 AA.
AC Q8CHN3; Q8CHN1;
DT 27-JUN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=WAP four-disulfide core domain protein 2;
DE AltName: Full=Epididymal secretory protein 4;
DE Short=RE4;
DE Flags: Precursor;
GN Name=Wfdc2;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=Sprague-Dawley; TISSUE=Epididymis;
RA Kaeppler-Hanno K., Yeung C.H., Kascheike I., Kirchhoff C.;
RT "Identification and characterization of rodent epididymal cDNAs by homology
RT to human and canine counterparts.";
RL Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Broad range protease inhibitor. {ECO:0000250}.
CC -!- SUBUNIT: Homotrimer; disulfide-linked. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8CHN3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8CHN3-2; Sequence=VSP_007673;
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DR EMBL; AJ515239; CAD56201.1; -; mRNA.
DR EMBL; AJ515241; CAD56203.1; -; mRNA.
DR RefSeq; NP_775132.1; NM_173109.1. [Q8CHN3-1]
DR AlphaFoldDB; Q8CHN3; -.
DR SMR; Q8CHN3; -.
DR STRING; 10116.ENSRNOP00000019804; -.
DR MEROPS; I17.004; -.
DR PaxDb; Q8CHN3; -.
DR Ensembl; ENSRNOT00000110333; ENSRNOP00000077787; ENSRNOG00000014739. [Q8CHN3-2]
DR GeneID; 286888; -.
DR KEGG; rno:286888; -.
DR UCSC; RGD:628757; rat. [Q8CHN3-1]
DR CTD; 10406; -.
DR RGD; 628757; Wfdc2.
DR VEuPathDB; HostDB:ENSRNOG00000014739; -.
DR eggNOG; ENOG502SA8J; Eukaryota.
DR GeneTree; ENSGT00730000111410; -.
DR HOGENOM; CLU_105901_3_0_1; -.
DR InParanoid; Q8CHN3; -.
DR OMA; NEKQGSC; -.
DR OrthoDB; 1409658at2759; -.
DR PhylomeDB; Q8CHN3; -.
DR PRO; PR:Q8CHN3; -.
DR Proteomes; UP000002494; Chromosome 3.
DR Bgee; ENSRNOG00000014739; Expressed in lung and 19 other tissues.
DR Genevisible; Q8CHN3; RN.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0019828; F:aspartic-type endopeptidase inhibitor activity; ISO:RGD.
DR GO; GO:0004869; F:cysteine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; ISO:RGD.
DR GO; GO:0019731; P:antibacterial humoral response; IBA:GO_Central.
DR GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR Gene3D; 4.10.75.10; -; 2.
DR InterPro; IPR036645; Elafin-like_sf.
DR InterPro; IPR008197; WAP_dom.
DR Pfam; PF00095; WAP; 2.
DR PRINTS; PR00003; 4DISULPHCORE.
DR SMART; SM00217; WAP; 2.
DR SUPFAM; SSF57256; SSF57256; 2.
DR PROSITE; PS51390; WAP; 2.
PE 2: Evidence at transcript level;
KW Alternative splicing; Aspartic protease inhibitor; Disulfide bond;
KW Protease inhibitor; Reference proteome; Repeat; Secreted;
KW Serine protease inhibitor; Signal; Thiol protease inhibitor.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..168
FT /note="WAP four-disulfide core domain protein 2"
FT /id="PRO_0000041374"
FT DOMAIN 31..74
FT /note="WAP 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DOMAIN 119..167
FT /note="WAP 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT REGION 100..123
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 36..62
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 45..66
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 49..61
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 55..70
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 126..154
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 137..158
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 141..153
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 147..163
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT VAR_SEQ 27..74
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.1"
FT /id="VSP_007673"
SQ SEQUENCE 168 AA; 17448 MW; 02AD0A718CAC1C9C CRC64;
MPACRLCLLA TGLLLGLLLF TPLSATGTRA EKPGVCPQLE PITDCVKACI LDNDCQDNYK
CCQAGCGSVC SKPNGLSEGK LSRTATGTTT LSAGLARTSP LSRGQVSTKP PVVTKEGGNG
EKQGTCPSVD FPKLGLCEDQ CQMDSQCSGN MKCCRNGCGK MGCTTPKF
