WFDC5_HUMAN
ID WFDC5_HUMAN Reviewed; 224 AA.
AC Q8TCV5; Q5H981; Q6UWE4;
DT 06-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=WAP four-disulfide core domain protein 5;
DE AltName: Full=Putative protease inhibitor WAP1;
DE AltName: Full=p53-responsive gene 5 protein;
DE Flags: Precursor;
GN Name=WFDC5; Synonyms=PRG5, WAP1; ORFNames=UNQ3036/PRO9842;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX PubMed=12206714; DOI=10.1042/bj20020869;
RA Clauss A., Lilja H., Lundwall A.;
RT "A locus on human chromosome 20 contains several genes expressing protease
RT inhibitor domains with homology to whey acidic protein.";
RL Biochem. J. 368:233-242(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT TYR-97.
RX PubMed=12975309; DOI=10.1101/gr.1293003;
RA Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J.,
RA Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P.,
RA Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A.,
RA Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D.,
RA Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L.,
RA Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C.,
RA Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J.,
RA Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
RT "The secreted protein discovery initiative (SPDI), a large-scale effort to
RT identify novel human secreted and transmembrane proteins: a bioinformatics
RT assessment.";
RL Genome Res. 13:2265-2270(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11780052; DOI=10.1038/414865a;
RA Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R.,
RA Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L.,
RA Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P.,
RA Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., Buck D., Burrill W.D.,
RA Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G.,
RA Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E.,
RA Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D.,
RA Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P.,
RA Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E.,
RA Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J.,
RA Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D.,
RA Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S.,
RA Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D.,
RA Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A.,
RA Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T.,
RA Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I.,
RA Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M.,
RA Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D.,
RA Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M.,
RA Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A.,
RA Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L.,
RA Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L.,
RA Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 20.";
RL Nature 414:865-871(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 145-224 (ISOFORM 1), AND INDUCTION.
RX PubMed=10441517; DOI=10.1006/bbrc.1999.1123;
RA Horikoshi N., Cong J., Kley N., Shenk T.;
RT "Isolation of differentially expressed cDNAs from p53-dependent apoptotic
RT cells: activation of the human homologue of the Drosophila peroxidasin
RT gene.";
RL Biochem. Biophys. Res. Commun. 261:864-869(1999).
CC -!- FUNCTION: Putative acid-stable proteinase inhibitor.
CC -!- INTERACTION:
CC Q8TCV5; Q12797-6: ASPH; NbExp=3; IntAct=EBI-12175871, EBI-12092171;
CC Q8TCV5; Q8WUW1: BRK1; NbExp=3; IntAct=EBI-12175871, EBI-2837444;
CC Q8TCV5; P27658: COL8A1; NbExp=3; IntAct=EBI-12175871, EBI-747133;
CC Q8TCV5; Q2WGJ6: KLHL38; NbExp=3; IntAct=EBI-12175871, EBI-6426443;
CC Q8TCV5; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-12175871, EBI-16439278;
CC Q8TCV5; O43765: SGTA; NbExp=3; IntAct=EBI-12175871, EBI-347996;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8TCV5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8TCV5-2; Sequence=VSP_006756;
CC -!- INDUCTION: By p53/TP53. {ECO:0000269|PubMed:10441517}.
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DR EMBL; AY038181; AAK72468.1; -; mRNA.
DR EMBL; AY358822; AAQ89181.1; -; mRNA.
DR EMBL; Z93016; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC039173; AAH39173.1; -; mRNA.
DR EMBL; AF147078; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS33475.1; -. [Q8TCV5-2]
DR RefSeq; NP_663627.1; NM_145652.3. [Q8TCV5-2]
DR RefSeq; XP_011526903.1; XM_011528601.1. [Q8TCV5-2]
DR RefSeq; XP_011526904.1; XM_011528602.1. [Q8TCV5-2]
DR AlphaFoldDB; Q8TCV5; -.
DR SMR; Q8TCV5; -.
DR BioGRID; 127236; 12.
DR IntAct; Q8TCV5; 6.
DR STRING; 9606.ENSP00000361875; -.
DR MEROPS; I17.003; -.
DR BioMuta; WFDC5; -.
DR DMDM; 26401313; -.
DR MassIVE; Q8TCV5; -.
DR PaxDb; Q8TCV5; -.
DR PeptideAtlas; Q8TCV5; -.
DR PRIDE; Q8TCV5; -.
DR ProteomicsDB; 74178; -. [Q8TCV5-1]
DR ProteomicsDB; 74179; -. [Q8TCV5-2]
DR Antibodypedia; 27523; 140 antibodies from 17 providers.
DR DNASU; 149708; -.
DR Ensembl; ENST00000307971.7; ENSP00000312381.4; ENSG00000175121.11. [Q8TCV5-1]
DR Ensembl; ENST00000372789.5; ENSP00000361875.4; ENSG00000175121.11. [Q8TCV5-2]
DR GeneID; 149708; -.
DR KEGG; hsa:149708; -.
DR MANE-Select; ENST00000372789.6; ENSP00000361875.4; NM_145652.4; NP_663627.1. [Q8TCV5-2]
DR UCSC; uc002xne.2; human. [Q8TCV5-1]
DR CTD; 149708; -.
DR DisGeNET; 149708; -.
DR GeneCards; WFDC5; -.
DR HGNC; HGNC:20477; WFDC5.
DR HPA; ENSG00000175121; Tissue enriched (skin).
DR MIM; 605161; gene.
DR neXtProt; NX_Q8TCV5; -.
DR OpenTargets; ENSG00000175121; -.
DR PharmGKB; PA134885667; -.
DR VEuPathDB; HostDB:ENSG00000175121; -.
DR eggNOG; ENOG502S99V; Eukaryota.
DR GeneTree; ENSGT00730000111369; -.
DR HOGENOM; CLU_105901_2_0_1; -.
DR InParanoid; Q8TCV5; -.
DR OMA; KRCCPGA; -.
DR OrthoDB; 1560605at2759; -.
DR PhylomeDB; Q8TCV5; -.
DR TreeFam; TF338375; -.
DR PathwayCommons; Q8TCV5; -.
DR SignaLink; Q8TCV5; -.
DR BioGRID-ORCS; 149708; 7 hits in 1057 CRISPR screens.
DR GeneWiki; WFDC5; -.
DR GenomeRNAi; 149708; -.
DR Pharos; Q8TCV5; Tbio.
DR PRO; PR:Q8TCV5; -.
DR Proteomes; UP000005640; Chromosome 20.
DR RNAct; Q8TCV5; protein.
DR Bgee; ENSG00000175121; Expressed in upper arm skin and 88 other tissues.
DR Genevisible; Q8TCV5; HS.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IBA:GO_Central.
DR GO; GO:0019731; P:antibacterial humoral response; IBA:GO_Central.
DR GO; GO:0045087; P:innate immune response; IBA:GO_Central.
DR Gene3D; 4.10.75.10; -; 2.
DR InterPro; IPR036645; Elafin-like_sf.
DR InterPro; IPR008197; WAP_dom.
DR Pfam; PF00095; WAP; 2.
DR PRINTS; PR00003; 4DISULPHCORE.
DR SMART; SM00217; WAP; 2.
DR SUPFAM; SSF57256; SSF57256; 2.
DR PROSITE; PS51390; WAP; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Disulfide bond; Protease inhibitor;
KW Reference proteome; Repeat; Secreted; Serine protease inhibitor; Signal.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..224
FT /note="WAP four-disulfide core domain protein 5"
FT /id="PRO_0000041382"
FT DOMAIN 27..73
FT /note="WAP 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DOMAIN 74..121
FT /note="WAP 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 34..62
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 41..66
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 49..61
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 55..70
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 81..109
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 88..113
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 96..108
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 102..117
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT VAR_SEQ 124..224
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:12206714,
FT ECO:0000303|PubMed:12975309, ECO:0000303|PubMed:15489334"
FT /id="VSP_006756"
FT VARIANT 97
FT /note="H -> Y (in dbSNP:rs17422688)"
FT /evidence="ECO:0000269|PubMed:12975309"
FT /id="VAR_057474"
FT CONFLICT 172
FT /note="Q -> R (in Ref. 5; AF147078)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 224 AA; 24238 MW; 305CC59024058F15 CRC64;
MRTQSLLLLG ALLAVGSQLP AVFGRKKGEK SGGCPPDDGP CLLSVPDQCV EDSQCPLTRK
CCYRACFRQC VPRVSVKLGS CPEDQLRCLS PMNHLCHKDS DCSGKKRCCH SACGRDCRDP
ARGTAPGCPG QANSDLGSVA LHLSWGPTER VHDGRPGALP AGQHYLYQRW FQPSDNHWPA
DTSLQPIHPW FLLLGVKVHS LSSEEGLCIT PVLCTTAIRA SHPS
