WFDC8_HUMAN
ID WFDC8_HUMAN Reviewed; 241 AA.
AC Q8IUA0; E1P623; Q5TDV2; Q96A34;
DT 04-APR-2003, integrated into UniProtKB/Swiss-Prot.
DT 20-FEB-2007, sequence version 2.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=WAP four-disulfide core domain protein 8;
DE AltName: Full=Putative protease inhibitor WAP8;
DE Flags: Precursor;
GN Name=WFDC8; Synonyms=C20orf170, WAP8;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT SER-137.
RX PubMed=12206714; DOI=10.1042/bj20020869;
RA Clauss A., Lilja H., Lundwall A.;
RT "A locus on human chromosome 20 contains several genes expressing protease
RT inhibitor domains with homology to whey acidic protein.";
RL Biochem. J. 368:233-242(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=11780052; DOI=10.1038/414865a;
RA Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R.,
RA Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L.,
RA Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P.,
RA Bird C.P., Blakey S.E., Bridgeman A.M., Brown A.J., Buck D., Burrill W.D.,
RA Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G.,
RA Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E.,
RA Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D.,
RA Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P.,
RA Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E.,
RA Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J.,
RA Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D.,
RA Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S.,
RA Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D.,
RA Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A.,
RA Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T.,
RA Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I.,
RA Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M.,
RA Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D.,
RA Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M.,
RA Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A.,
RA Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L.,
RA Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L.,
RA Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.;
RT "The DNA sequence and comparative analysis of human chromosome 20.";
RL Nature 414:865-871(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed ubiquitously, the highest levels are
CC found in the epididymis followed by testis and trachea.
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DR EMBL; AF492015; AAN70997.1; -; mRNA.
DR EMBL; AF492016; AAN70998.1; -; mRNA.
DR EMBL; AL591715; CAC39449.1; -; mRNA.
DR EMBL; AL121778; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL031663; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471077; EAW75828.1; -; Genomic_DNA.
DR EMBL; CH471077; EAW75829.1; -; Genomic_DNA.
DR CCDS; CCDS13361.1; -.
DR RefSeq; NP_570966.2; NM_130896.2.
DR RefSeq; NP_852611.2; NM_181510.2.
DR AlphaFoldDB; Q8IUA0; -.
DR BioGRID; 124676; 16.
DR STRING; 9606.ENSP00000361735; -.
DR MEROPS; I02.971; -.
DR iPTMnet; Q8IUA0; -.
DR PhosphoSitePlus; Q8IUA0; -.
DR BioMuta; WFDC8; -.
DR DMDM; 126302615; -.
DR MassIVE; Q8IUA0; -.
DR PaxDb; Q8IUA0; -.
DR PeptideAtlas; Q8IUA0; -.
DR PRIDE; Q8IUA0; -.
DR ProteomicsDB; 70526; -.
DR Antibodypedia; 63192; 36 antibodies from 8 providers.
DR DNASU; 90199; -.
DR Ensembl; ENST00000289953.3; ENSP00000289953.2; ENSG00000158901.12.
DR Ensembl; ENST00000357199.8; ENSP00000361735.3; ENSG00000158901.12.
DR GeneID; 90199; -.
DR KEGG; hsa:90199; -.
DR MANE-Select; ENST00000289953.3; ENSP00000289953.2; NM_130896.3; NP_570966.2.
DR UCSC; uc002xow.4; human.
DR CTD; 90199; -.
DR GeneCards; WFDC8; -.
DR HGNC; HGNC:16163; WFDC8.
DR HPA; ENSG00000158901; Tissue enriched (epididymis).
DR neXtProt; NX_Q8IUA0; -.
DR OpenTargets; ENSG00000158901; -.
DR PharmGKB; PA25713; -.
DR VEuPathDB; HostDB:ENSG00000158901; -.
DR eggNOG; KOG4295; Eukaryota.
DR GeneTree; ENSGT00940000162037; -.
DR HOGENOM; CLU_092095_0_0_1; -.
DR InParanoid; Q8IUA0; -.
DR OMA; CGLKCLE; -.
DR OrthoDB; 1631923at2759; -.
DR PhylomeDB; Q8IUA0; -.
DR TreeFam; TF335948; -.
DR PathwayCommons; Q8IUA0; -.
DR BioGRID-ORCS; 90199; 5 hits in 1056 CRISPR screens.
DR ChiTaRS; WFDC8; human.
DR GenomeRNAi; 90199; -.
DR Pharos; Q8IUA0; Tdark.
DR PRO; PR:Q8IUA0; -.
DR Proteomes; UP000005640; Chromosome 20.
DR RNAct; Q8IUA0; protein.
DR Bgee; ENSG00000158901; Expressed in corpus epididymis and 27 other tissues.
DR ExpressionAtlas; Q8IUA0; baseline and differential.
DR Genevisible; Q8IUA0; HS.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR CDD; cd00109; KU; 1.
DR Gene3D; 4.10.410.10; -; 1.
DR Gene3D; 4.10.75.10; -; 3.
DR InterPro; IPR036645; Elafin-like_sf.
DR InterPro; IPR002223; Kunitz_BPTI.
DR InterPro; IPR036880; Kunitz_BPTI_sf.
DR InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR InterPro; IPR008197; WAP_dom.
DR InterPro; IPR042931; WFDC8.
DR PANTHER; PTHR47769; PTHR47769; 1.
DR Pfam; PF00014; Kunitz_BPTI; 1.
DR Pfam; PF00095; WAP; 3.
DR PRINTS; PR00003; 4DISULPHCORE.
DR PRINTS; PR00759; BASICPTASE.
DR SMART; SM00131; KU; 1.
DR SMART; SM00217; WAP; 3.
DR SUPFAM; SSF57256; SSF57256; 3.
DR SUPFAM; SSF57362; SSF57362; 1.
DR PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR PROSITE; PS50279; BPTI_KUNITZ_2; 1.
DR PROSITE; PS51390; WAP; 3.
PE 2: Evidence at transcript level;
KW Disulfide bond; Protease inhibitor; Reference proteome; Repeat; Secreted;
KW Serine protease inhibitor; Signal.
FT SIGNAL 1..38
FT /evidence="ECO:0000255"
FT CHAIN 39..241
FT /note="WAP four-disulfide core domain protein 8"
FT /id="PRO_0000041384"
FT DOMAIN 44..91
FT /note="WAP 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DOMAIN 95..145
FT /note="BPTI/Kunitz inhibitor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT DOMAIN 147..194
FT /note="WAP 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DOMAIN 195..240
FT /note="WAP 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 51..79
FT /evidence="ECO:0000250"
FT DISULFID 58..83
FT /evidence="ECO:0000250"
FT DISULFID 66..78
FT /evidence="ECO:0000250"
FT DISULFID 72..87
FT /evidence="ECO:0000250"
FT DISULFID 95..145
FT /evidence="ECO:0000250"
FT DISULFID 104..128
FT /evidence="ECO:0000250"
FT DISULFID 120..141
FT /evidence="ECO:0000250"
FT DISULFID 154..182
FT /evidence="ECO:0000250"
FT DISULFID 165..186
FT /evidence="ECO:0000250"
FT DISULFID 169..181
FT /evidence="ECO:0000250"
FT DISULFID 175..190
FT /evidence="ECO:0000250"
FT DISULFID 201..229
FT /evidence="ECO:0000250"
FT DISULFID 208..232
FT /evidence="ECO:0000250"
FT DISULFID 216..228
FT /evidence="ECO:0000250"
FT DISULFID 222..236
FT /evidence="ECO:0000250"
FT VARIANT 96
FT /note="M -> T (in dbSNP:rs2272955)"
FT /id="VAR_021910"
FT VARIANT 137
FT /note="N -> S (in dbSNP:rs2250860)"
FT /evidence="ECO:0000269|PubMed:12206714"
FT /id="VAR_030861"
SQ SEQUENCE 241 AA; 27824 MW; 3EF717C0DC1F54C2 CRC64;
MWTVRTEGGH FPLHSPTFSW RNVAFLLLLS LALEWTSAML TKKIKHKPGL CPKERLTCTT
ELPDSCNTDF DCKEYQKCCF FACQKKCMDP FQEPCMLPVR HGNCNHEAQR WHFDFKNYRC
TPFKYRGCEG NANNFLNEDA CRTACMLIVK DGQCPLFPFT ERKECPPSCH SDIDCPQTDK
CCESRCGFVC ARAWTVKKGF CPRKPLLCTK IDKPKCLQDE ECPLVEKCCS HCGLKCMDPR
R
