WFDC8_MOUSE
ID WFDC8_MOUSE Reviewed; 273 AA.
AC Q4KUS1;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 02-AUG-2005, sequence version 1.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=WAP four-disulfide core domain protein 8;
DE AltName: Full=Putative protease inhibitor WAP8C;
GN Name=Wfdc8; Synonyms=Gm706;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=15950183; DOI=10.1016/j.bbrc.2005.05.125;
RA Clauss A., Lilja H., Lundwall A.;
RT "The evolution of a genetic locus encoding small serine proteinase
RT inhibitors.";
RL Biochem. Biophys. Res. Commun. 333:383-389(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC protein {ECO:0000305}.
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DR EMBL; AY757956; AAU93641.1; -; mRNA.
DR EMBL; AL591478; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR CCDS; CCDS38324.1; -.
DR RefSeq; NP_001074019.1; NM_001080550.2.
DR AlphaFoldDB; Q4KUS1; -.
DR SMR; Q4KUS1; -.
DR STRING; 10090.ENSMUSP00000104962; -.
DR MEROPS; I02.978; -.
DR PhosphoSitePlus; Q4KUS1; -.
DR PaxDb; Q4KUS1; -.
DR PRIDE; Q4KUS1; -.
DR ProteomicsDB; 299766; -.
DR Antibodypedia; 63192; 36 antibodies from 8 providers.
DR DNASU; 277343; -.
DR Ensembl; ENSMUST00000109338; ENSMUSP00000104962; ENSMUSG00000070533.
DR GeneID; 277343; -.
DR KEGG; mmu:277343; -.
DR UCSC; uc008nvk.2; mouse.
DR CTD; 90199; -.
DR MGI; MGI:2685552; Wfdc8.
DR VEuPathDB; HostDB:ENSMUSG00000070533; -.
DR eggNOG; KOG4295; Eukaryota.
DR GeneTree; ENSGT00940000162037; -.
DR HOGENOM; CLU_092095_0_0_1; -.
DR InParanoid; Q4KUS1; -.
DR OMA; CGLKCLE; -.
DR PhylomeDB; Q4KUS1; -.
DR TreeFam; TF335948; -.
DR BioGRID-ORCS; 277343; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Wfdc8; mouse.
DR PRO; PR:Q4KUS1; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q4KUS1; protein.
DR Bgee; ENSMUSG00000070533; Expressed in testis and 4 other tissues.
DR ExpressionAtlas; Q4KUS1; baseline and differential.
DR GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-KW.
DR CDD; cd00109; KU; 1.
DR Gene3D; 4.10.410.10; -; 1.
DR Gene3D; 4.10.75.10; -; 3.
DR InterPro; IPR036645; Elafin-like_sf.
DR InterPro; IPR002223; Kunitz_BPTI.
DR InterPro; IPR036880; Kunitz_BPTI_sf.
DR InterPro; IPR020901; Prtase_inh_Kunz-CS.
DR InterPro; IPR008197; WAP_dom.
DR InterPro; IPR042931; WFDC8.
DR PANTHER; PTHR47769; PTHR47769; 1.
DR Pfam; PF00014; Kunitz_BPTI; 1.
DR Pfam; PF00095; WAP; 3.
DR PRINTS; PR00003; 4DISULPHCORE.
DR PRINTS; PR00759; BASICPTASE.
DR SMART; SM00131; KU; 1.
DR SMART; SM00217; WAP; 3.
DR SUPFAM; SSF57256; SSF57256; 3.
DR SUPFAM; SSF57362; SSF57362; 1.
DR PROSITE; PS00280; BPTI_KUNITZ_1; 1.
DR PROSITE; PS50279; BPTI_KUNITZ_2; 1.
DR PROSITE; PS51390; WAP; 3.
PE 2: Evidence at transcript level;
KW Disulfide bond; Membrane; Protease inhibitor; Reference proteome; Repeat;
KW Serine protease inhibitor; Transmembrane; Transmembrane helix.
FT CHAIN 1..273
FT /note="WAP four-disulfide core domain protein 8"
FT /id="PRO_0000307717"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 76..123
FT /note="WAP 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DOMAIN 127..177
FT /note="BPTI/Kunitz inhibitor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00031"
FT DOMAIN 179..226
FT /note="WAP 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DOMAIN 228..272
FT /note="WAP 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00722"
FT DISULFID 83..111
FT /evidence="ECO:0000250"
FT DISULFID 90..115
FT /evidence="ECO:0000250"
FT DISULFID 98..110
FT /evidence="ECO:0000250"
FT DISULFID 104..119
FT /evidence="ECO:0000250"
FT DISULFID 127..177
FT /evidence="ECO:0000250"
FT DISULFID 136..160
FT /evidence="ECO:0000250"
FT DISULFID 152..173
FT /evidence="ECO:0000250"
FT DISULFID 186..214
FT /evidence="ECO:0000250"
FT DISULFID 197..218
FT /evidence="ECO:0000250"
FT DISULFID 201..213
FT /evidence="ECO:0000250"
FT DISULFID 207..222
FT /evidence="ECO:0000250"
FT DISULFID 233..261
FT /evidence="ECO:0000250"
FT DISULFID 240..264
FT /evidence="ECO:0000250"
FT DISULFID 248..260
FT /evidence="ECO:0000250"
FT DISULFID 254..268
FT /evidence="ECO:0000250"
SQ SEQUENCE 273 AA; 31279 MW; 9FDF4619B9EF1513 CRC64;
MAKCGVPQRV PGDCFRPVST RHPRQIPSCF SICFLTHEAQ SLRALAHSWW SGALLLLLLF
LFLSLEQTST SYNAKIKQKV GECPRQRLEC RNESLSSCKT DFNCKAHFKC CQFACGRKCM
DPYEEPCMLP SDKGNCQDIL TRWYFDSQKH QCRAFLYSGC RGNANNFLTK TDCRNACMFV
EKKGQCPLFP FQMRMECPAS CKNDMDCPEK EKCCESRCGF ICARVWLVKT GFCPRKPIVC
SKIDKPKCLQ DIDCPLDEKC CTRCGLKCLK PRH
