WGN_DROME
ID WGN_DROME Reviewed; 343 AA.
AC Q9VWS4; Q7Z1X1;
DT 09-DEC-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Tumor necrosis factor receptor superfamily member wengen {ECO:0000303|PubMed:12084706};
DE Flags: Precursor;
GN Name=wgn {ECO:0000312|FlyBase:FBgn0030941};
GN Synonyms=Vader {ECO:0000312|EMBL:AAM48414.1};
GN ORFNames=CG6531 {ECO:0000312|FlyBase:FBgn0030941};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|EMBL:BAC01264.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH EGR, SUBCELLULAR
RP LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=12084706; DOI=10.1074/jbc.c200324200;
RA Kanda H., Igaki T., Kanuka H., Yagi T., Miura M.;
RT "Wengen, a member of the Drosophila tumor necrosis factor receptor
RT superfamily, is required for Eiger signaling.";
RL J. Biol. Chem. 277:28372-28375(2002).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000312|EMBL:AAM48414.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAM48414.1};
RC TISSUE=Embryo {ECO:0000312|EMBL:AAM48414.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5] {ECO:0000312|EMBL:AAM66764.1}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 14-343, FUNCTION, INTERACTION WITH TRAF6, AND
RP TISSUE SPECIFICITY.
RX PubMed=12894227; DOI=10.1038/sj.onc.1206715;
RA Kauppila S., Maaty W.S., Chen P., Tomar R.S., Eby M.T., Chapo J., Chew S.,
RA Rathore N., Zachariah S., Sinha S.K., Abrams J.M., Chaudhary P.M.;
RT "Eiger and its receptor, Wengen, comprise a TNF-like system in
RT Drosophila.";
RL Oncogene 22:4860-4867(2003).
RN [6] {ECO:0000305}
RP FUNCTION.
RX PubMed=19289090; DOI=10.1016/j.devcel.2009.01.002;
RA Igaki T., Pastor-Pareja J.C., Aonuma H., Miura M., Xu T.;
RT "Intrinsic tumor suppression and epithelial maintenance by endocytic
RT activation of Eiger/TNF signaling in Drosophila.";
RL Dev. Cell 16:458-465(2009).
RN [7] {ECO:0000305}
RP FUNCTION.
RX PubMed=19375319; DOI=10.1016/j.cub.2009.03.062;
RA Babcock D.T., Landry C., Galko M.J.;
RT "Cytokine signaling mediates UV-induced nociceptive sensitization in
RT Drosophila larvae.";
RL Curr. Biol. 19:799-806(2009).
RN [8] {ECO:0000305}
RP FUNCTION, INTERACTION WITH MOE, AND TISSUE SPECIFICITY.
RX PubMed=23544124; DOI=10.1371/journal.pone.0060091;
RA Ruan W., Unsain N., Desbarats J., Fon E.A., Barker P.A.;
RT "Wengen, the sole tumour necrosis factor receptor in Drosophila,
RT collaborates with moesin to control photoreceptor axon targeting during
RT development.";
RL PLoS ONE 8:E60091-E60091(2013).
CC -!- FUNCTION: Receptor for egr (PubMed:12084706, PubMed:12894227). Involved
CC in induction of apoptosis by triggering JNK signaling
CC (PubMed:12894227). Mediates the tumor suppressor activity of egr which
CC eliminates oncogenic cells from epithelia, thereby maintaining
CC epithelial integrity (PubMed:19289090). Following UV-induced epidermal
CC damage, binds to egr released from apoptotic epidermal cells and plays
CC a role in development of thermal allodynia, a responsiveness to
CC subthreshold thermal stimuli which are not normally perceived as
CC noxious (PubMed:19375319). Together with Moe, involved in control of
CC axon targeting of R8 and R2-R5 photoreceptors, independent of egr
CC (PubMed:23544124). {ECO:0000269|PubMed:12084706,
CC ECO:0000269|PubMed:12894227, ECO:0000269|PubMed:19289090,
CC ECO:0000269|PubMed:19375319, ECO:0000269|PubMed:23544124}.
CC -!- SUBUNIT: Interacts with egr (PubMed:12084706). Interacts with Traf6
CC (PubMed:12894227). Interacts with Moe (PubMed:23544124).
CC {ECO:0000269|PubMed:12084706, ECO:0000269|PubMed:12894227,
CC ECO:0000269|PubMed:23544124}.
CC -!- INTERACTION:
CC Q9VWS4; Q8MUJ1: egr; NbExp=3; IntAct=EBI-161953, EBI-108054;
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12084706};
CC Single-pass type I membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: In third instar larvae, detected in photoreceptor
CC neurons, including R8 photoreceptors, and also in adult head (at
CC protein level) (PubMed:23544124). Expression appears to be very low or
CC undetectable in pregastrulating embryos. During gastrulation, detected
CC in the inner layer of embryonic tissue corresponding to the presumptive
CC mesoderm. At germ band extended stages, continues to accumulate in the
CC mesodermal segments of the embryo. In later-staged embryos (stages
CC 15/16), detected in subsets of cells within the condensing nerve cord
CC (PubMed:12894227). {ECO:0000269|PubMed:12894227,
CC ECO:0000269|PubMed:23544124}.
CC -!- DEVELOPMENTAL STAGE: Present at all stages of development with
CC expression detected in embryo, larva, pupa and adult.
CC {ECO:0000269|PubMed:12084706}.
CC -!- MISCELLANEOUS: 'wengen' is named after a village at the foot of the
CC Eiger mountain after which the egr gene is named.
CC {ECO:0000303|PubMed:12084706}.
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DR EMBL; AB085747; BAC01264.1; -; mRNA.
DR EMBL; AE003509; AAF48864.2; -; Genomic_DNA.
DR EMBL; AY118385; AAM48414.1; -; mRNA.
DR EMBL; AY115552; AAM66764.1; -; mRNA.
DR RefSeq; NP_728186.1; NM_167626.2.
DR AlphaFoldDB; Q9VWS4; -.
DR IntAct; Q9VWS4; 2.
DR STRING; 7227.FBpp0074388; -.
DR PaxDb; Q9VWS4; -.
DR DNASU; 32849; -.
DR EnsemblMetazoa; FBtr0074617; FBpp0074388; FBgn0030941.
DR GeneID; 32849; -.
DR KEGG; dme:Dmel_CG6531; -.
DR UCSC; CG6531-RA; d. melanogaster.
DR CTD; 32849; -.
DR FlyBase; FBgn0030941; wgn.
DR VEuPathDB; VectorBase:FBgn0030941; -.
DR eggNOG; ENOG502SCIN; Eukaryota.
DR HOGENOM; CLU_069708_0_0_1; -.
DR InParanoid; Q9VWS4; -.
DR OMA; TSCQGEM; -.
DR PhylomeDB; Q9VWS4; -.
DR BioGRID-ORCS; 32849; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 32849; -.
DR PRO; PR:Q9VWS4; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0030941; Expressed in crop (Drosophila) and 54 other tissues.
DR ExpressionAtlas; Q9VWS4; baseline and differential.
DR GO; GO:0009986; C:cell surface; IDA:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:FlyBase.
DR GO; GO:0005031; F:tumor necrosis factor receptor activity; IDA:FlyBase.
DR GO; GO:0006915; P:apoptotic process; IDA:UniProtKB.
DR GO; GO:1900074; P:negative regulation of neuromuscular synaptic transmission; IGI:FlyBase.
DR GO; GO:0070050; P:neuron cellular homeostasis; IGI:FlyBase.
DR GO; GO:0072499; P:photoreceptor cell axon guidance; IMP:FlyBase.
DR GO; GO:0010942; P:positive regulation of cell death; IGI:FlyBase.
DR GO; GO:0033209; P:tumor necrosis factor-mediated signaling pathway; IGI:FlyBase.
DR InterPro; IPR001368; TNFR/NGFR_Cys_rich_reg.
DR SMART; SM00208; TNFR; 1.
DR PROSITE; PS50050; TNFR_NGFR_2; 1.
PE 1: Evidence at protein level;
KW Apoptosis; Cell membrane; Coiled coil; Disulfide bond; Membrane;
KW Neurogenesis; Receptor; Reference proteome; Signal; Transmembrane;
KW Transmembrane helix.
FT SIGNAL 1..76
FT /evidence="ECO:0000255"
FT CHAIN 77..343
FT /note="Tumor necrosis factor receptor superfamily member
FT wengen"
FT /evidence="ECO:0000255"
FT /id="PRO_0000434983"
FT TOPO_DOM 77..201
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 223..343
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT REPEAT 99..137
FT /note="TNFR-Cys"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00206"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 74..96
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 225..257
FT /evidence="ECO:0000255"
FT COMPBIAS 26..41
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT DISULFID 100..113
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00206"
FT DISULFID 116..129
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00206"
FT DISULFID 119..137
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00206"
SQ SEQUENCE 343 AA; 37829 MW; BD5E6F5318A82170 CRC64;
MMPPRLPGGH GGAMRSRSSS SGHHLNTTFH KRRRRRQQHH IGGSHISISH GSYLALLLLS
TTCSLVATSA SSSSSAANTD IAPPDPPPVS QVSIASSSPC APQHWWDSQR DRCTPCTRCQ
GEMIPLRPCQ LHTDTICGSI YDLKIDWVVL AKTEPNWKER RKSSEYEHFE HNAPLQHLTH
EQLQQLHEEA AAAWVLDWQT GVLYVAVLTC LVFFSVAACI LIHHMRQWRR MERRLDQDVE
ELSTKLMAKL AEVQSLDGGT FFIGNADALR GLPASAATTH PATTQSGIFQ PQHVLLPEKR
GKHQERRILK TLQPGNVYIE ESNAGLGGMG VGLGVRGCSG LKG
