ID   WHB5B_MYCTO             Reviewed;         139 AA.
AC   P0DKR7;
DT   28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2012, sequence version 1.
DT   03-AUG-2022, entry version 33.
DE   RecName: Full=Transcriptional regulator WhiB5;
GN   Name=whiB5; OrderedLocusNames=MT0025.1;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
RN   [2]
RP   INDUCTION.
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=16870781; DOI=10.1128/aac.00295-06;
RA   Geiman D.E., Raghunand T.R., Agarwal N., Bishai W.R.;
RT   "Differential gene expression in response to exposure to antimycobacterial
RT   agents and other stress conditions among seven Mycobacterium tuberculosis
RT   whiB-like genes.";
RL   Antimicrob. Agents Chemother. 50:2836-2841(2006).
RN   [3]
RP   INDUCTION.
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=22829866; DOI=10.1371/journal.pone.0037516;
RA   Larsson C., Luna B., Ammerman N.C., Maiga M., Agarwal N., Bishai W.R.;
RT   "Gene expression of Mycobacterium tuberculosis putative transcription
RT   factors whiB1-7 in redox environments.";
RL   PLoS ONE 7:E37516-E37516(2012).
CC   -!- FUNCTION: Acts as a transcriptional regulator. Probably redox-
CC       responsive. The apo- but not holo-form probably binds DNA (By
CC       similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster per subunit. Following nitrosylation of
CC       the [4Fe-4S] cluster binds 1 [4Fe-8(NO)] cluster per subunit.
CC       {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: Essentially constitutive during exponential and early
CC       stationary phases, decreases in late stationary phase. 3-fold induced
CC       by starvation, 3-fold by cumene hydroperoxide and 5-fold by diamide
CC       (oxidzing agents), 15-fold induced by SDS, 200-fold by heat shock.
CC       Repressed by SDS and diamide. Not induced by hypoxia, NO, cAMP or in
CC       mouse infection, slightly repressed in macrophage infection.
CC       {ECO:0000269|PubMed:16870781, ECO:0000269|PubMed:22829866}.
CC   -!- PTM: The Fe-S cluster can be nitrosylated by nitric oxide (NO).
CC       {ECO:0000250}.
CC   -!- PTM: Upon Fe-S cluster removal intramolecular disulfide bonds are
CC       formed. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the WhiB family. {ECO:0000305}.
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DR   EMBL; AE000516; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; WP_003400388.1; NZ_KK341227.1.
DR   AlphaFoldDB; P0DKR7; -.
DR   SMR; P0DKR7; -.
DR   GeneID; 45423982; -.
DR   PATRIC; fig|83331.31.peg.26; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0035731; F:dinitrosyl-iron complex binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01479; WhiB; 1.
DR   InterPro; IPR034768; 4FE4S_WBL.
DR   InterPro; IPR003482; Whib.
DR   Pfam; PF02467; Whib; 1.
DR   PROSITE; PS51674; 4FE4S_WBL; 1.
PE   2: Evidence at transcript level;
KW   4Fe-4S; Cytoplasm; Disulfide bond; DNA-binding; Iron; Iron-sulfur;
KW   Metal-binding; Transcription; Transcription regulation.
FT   CHAIN           1..139
FT                   /note="Transcriptional regulator WhiB5"
FT                   /id="PRO_0000420385"
FT   DOMAIN          4..77
FT                   /note="4Fe-4S Wbl-type"
FT   BINDING         5
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         41
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         45
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         53
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   139 AA;  15178 MW;  C4AFADECFC9DFDD2 CRC64;
     MAHPCATDPE LWFGYPDDDG SDGAAKARAY ERSATQARIQ CLRRCPLLQQ RRCAQHAVEH
     RVEYGVWAGI KLPGGQYRKR EQLAAAHDVL RRIAGGEINS RQLPDNAALL ARNEGLEVTP
     VPGVVVHLPI AQVGPQPAA