ID   WHB7B_MYCTO             Reviewed;          92 AA.
AC   Q8VJ53;
DT   28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT   28-NOV-2012, sequence version 2.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Probable transcriptional regulator WhiB7;
GN   Name=whiB7; OrderedLocusNames=MT3290.1;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
RN   [2]
RP   INDUCTION.
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=16870781; DOI=10.1128/aac.00295-06;
RA   Geiman D.E., Raghunand T.R., Agarwal N., Bishai W.R.;
RT   "Differential gene expression in response to exposure to antimycobacterial
RT   agents and other stress conditions among seven Mycobacterium tuberculosis
RT   whiB-like genes.";
RL   Antimicrob. Agents Chemother. 50:2836-2841(2006).
RN   [3]
RP   INDUCTION.
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=22829866; DOI=10.1371/journal.pone.0037516;
RA   Larsson C., Luna B., Ammerman N.C., Maiga M., Agarwal N., Bishai W.R.;
RT   "Gene expression of Mycobacterium tuberculosis putative transcription
RT   factors whiB1-7 in redox environments.";
RL   PLoS ONE 7:E37516-E37516(2012).
CC   -!- FUNCTION: Acts as a transcriptional regulator. Probably redox-
CC       responsive. The apo- but not holo-form probably binds DNA (By
CC       similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster per subunit. Following nitrosylation of
CC       the [4Fe-4S] cluster binds 1 [4Fe-8(NO)] cluster per subunit.
CC       {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: Weakly expressed in exponential phase, expression peaks as
CC       cells enter stationary phase then falls again. 30-fold induced by iron-
CC       starvation, 60-fold by heat shock, 70-fold by streptomycin and
CC       kanamycin. 2-fold repressed by ethanol and streptomycin. Slightly
CC       induced during entry into hypoxia, by NO, cAMP, in mouse infections and
CC       15-fold induced in macrophage infection. {ECO:0000269|PubMed:16870781,
CC       ECO:0000269|PubMed:22829866}.
CC   -!- PTM: The Fe-S cluster can be nitrosylated by nitric oxide (NO).
CC       {ECO:0000250}.
CC   -!- PTM: Upon Fe-S cluster removal intramolecular disulfide bonds are
CC       formed. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the WhiB family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK47632.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK47632.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_003899963.1; NZ_KK341227.1.
DR   AlphaFoldDB; Q8VJ53; -.
DR   SMR; Q8VJ53; -.
DR   EnsemblBacteria; AAK47632; AAK47632; MT3290.1.
DR   GeneID; 45427187; -.
DR   KEGG; mtc:MT3290.1; -.
DR   PATRIC; fig|83331.31.peg.3542; -.
DR   HOGENOM; CLU_106245_3_1_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0035731; F:dinitrosyl-iron complex binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01479; WhiB; 1.
DR   InterPro; IPR034768; 4FE4S_WBL.
DR   InterPro; IPR017956; AT_hook_DNA-bd_motif.
DR   InterPro; IPR003482; Whib.
DR   PANTHER; PTHR38839; PTHR38839; 1.
DR   Pfam; PF02178; AT_hook; 1.
DR   Pfam; PF02467; Whib; 1.
DR   PROSITE; PS51674; 4FE4S_WBL; 1.
PE   2: Evidence at transcript level;
KW   4Fe-4S; Cytoplasm; Disulfide bond; DNA-binding; Iron; Iron-sulfur;
KW   Metal-binding; Transcription; Transcription regulation.
FT   CHAIN           1..92
FT                   /note="Probable transcriptional regulator WhiB7"
FT                   /id="PRO_0000420388"
FT   DOMAIN          19..76
FT                   /note="4Fe-4S Wbl-type"
FT   DNA_BIND        80..91
FT                   /note="A.T hook"
FT   BINDING         20
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         43
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         46
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         52
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   92 AA;  10139 MW;  18EA51941235B9E8 CRC64;
     MSVLTVPRQT PRQRLPVLPC HVGDPDLWFA DTPAGLEVAK TLCVSCPIRR QCLAAALQRA
     EPWGVWGGEI FDQGSIVSHK RPRGRPRKDA VA