WHI4_YEAST
ID WHI4_YEAST Reviewed; 649 AA.
AC Q07655; D6VRD1;
DT 28-NOV-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 149.
DE RecName: Full=Protein WHI4;
GN Name=WHI4; OrderedLocusNames=YDL224C; ORFNames=D0839;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION.
RX PubMed=11290704; DOI=10.1093/genetics/157.4.1469;
RA Nash R.S., Volpe T., Futcher B.;
RT "Isolation and characterization of WHI3, a size-control gene of
RT Saccharomyces cerevisiae.";
RL Genetics 157:1469-1480(2001).
RN [4]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=14562095; DOI=10.1038/nature02026;
RA Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA Weissman J.S., O'Shea E.K.;
RT "Global analysis of protein localization in budding yeast.";
RL Nature 425:686-691(2003).
RN [5]
RP PHOSPHORYLATION.
RX PubMed=16172400; DOI=10.1073/pnas.0501046102;
RA Budovskaya Y.V., Stephan J.S., Deminoff S.J., Herman P.K.;
RT "An evolutionary proteomics approach identifies substrates of the cAMP-
RT dependent protein kinase.";
RL Proc. Natl. Acad. Sci. U.S.A. 102:13933-13938(2005).
RN [6]
RP INDUCTION.
RX PubMed=16278933; DOI=10.1002/yea.1302;
RA de Lichtenberg U., Wernersson R., Jensen T.S., Nielsen H.B., Fausboell A.,
RA Schmidt P., Hansen F.B., Knudsen S., Brunak S.;
RT "New weakly expressed cell cycle-regulated genes in yeast.";
RL Yeast 22:1191-1201(2005).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-22; SER-206; SER-258 AND
RP SER-283, AND IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- FUNCTION: Has a partially redundant function to WHI3, a dosage-
CC dependent modulator of cell size. {ECO:0000269|PubMed:11290704}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:14562095}.
CC -!- INDUCTION: Cell cycle-regulated. Expression peaks during S/G2 phase.
CC {ECO:0000269|PubMed:16278933}.
CC -!- PTM: Phosphorylated by PKA in vitro. {ECO:0000269|PubMed:16172400}.
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DR EMBL; Z74272; CAA98803.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11641.1; -; Genomic_DNA.
DR PIR; S67787; S67787.
DR RefSeq; NP_010057.1; NM_001180284.1.
DR AlphaFoldDB; Q07655; -.
DR SMR; Q07655; -.
DR BioGRID; 31822; 93.
DR DIP; DIP-4321N; -.
DR IntAct; Q07655; 8.
DR MINT; Q07655; -.
DR STRING; 4932.YDL224C; -.
DR iPTMnet; Q07655; -.
DR MaxQB; Q07655; -.
DR PaxDb; Q07655; -.
DR PRIDE; Q07655; -.
DR EnsemblFungi; YDL224C_mRNA; YDL224C; YDL224C.
DR GeneID; 851302; -.
DR KEGG; sce:YDL224C; -.
DR SGD; S000002383; WHI4.
DR VEuPathDB; FungiDB:YDL224C; -.
DR eggNOG; KOG0118; Eukaryota.
DR GeneTree; ENSGT00940000176750; -.
DR HOGENOM; CLU_018359_0_0_1; -.
DR InParanoid; Q07655; -.
DR OMA; THYANIL; -.
DR BioCyc; YEAST:G3O-29604-MON; -.
DR PRO; PR:Q07655; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q07655; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0003729; F:mRNA binding; ISS:SGD.
DR GO; GO:0061157; P:mRNA destabilization; IGI:SGD.
DR GO; GO:0008361; P:regulation of cell size; IGI:SGD.
DR Gene3D; 3.30.70.330; -; 1.
DR InterPro; IPR012677; Nucleotide-bd_a/b_plait_sf.
DR InterPro; IPR035979; RBD_domain_sf.
DR InterPro; IPR000504; RRM_dom.
DR Pfam; PF00076; RRM_1; 1.
DR SMART; SM00360; RRM; 1.
DR SUPFAM; SSF54928; SSF54928; 1.
DR PROSITE; PS50102; RRM; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Phosphoprotein; Reference proteome; RNA-binding.
FT CHAIN 1..649
FT /note="Protein WHI4"
FT /id="PRO_0000262756"
FT DOMAIN 533..625
FT /note="RRM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00176"
FT REGION 196..217
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 228..247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 438..461
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 604..649
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 616..649
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 22
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 206
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 258
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
FT MOD_RES 283
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19779198"
SQ SEQUENCE 649 AA; 70690 MW; 8A1E7DDED388B73C CRC64;
MSLVHNQTNL NESKFLIERA FSSSSETVPL SKEATYPMPT AYSFSAVRSN SETNIKRENP
QGFAKEPIMT SMLHNLTMST GKGNGNDVNS LAPHDVDVGP YCLLLRNLPK DITLRECYCI
FSLATGVSSI ELKRDDREPF NDNEKVVVVK FGSLSLVTHY ANILNSKSEI FGPSFPFRSH
IDVVNEQTQL PVSFQEHVSS GTTNSSPKNY QLSSSAQNEI QNQSFNTISY GKTSSSPLGP
SAAKPRPSLL SERSLRFSFN DPFGLETISQ RKESVPFLRN SISQHDLSNV TTTPVPAGMP
PQKDAGKSLL LLEKDEINES IWNGDELVND VGNSSFGASL QEPPMSSTPV MEWNASSTAN
IPLFQLSSQE NHQSNLLPPS HHSISQDVPH IQSQPNLNNS GVIHSATSLP HYHLLNQINA
STKTQSIQQS VSNVPSNLDL NLQTENGHPQ SSAPNGSSIF NNQKVNQGFL VSEQDTSTIS
RQKECSSTAS ASAFSKNNET NVAGSTTISQ ADLSLLAKVP PPANPADQNP PCNTLYVGNL
PPDATEQELR QLFSNQQGFR RLSFRNKMNS HGHGNGHGHG PICFVEFEDV SFATRALAEL
YGSQLPHPRP SLNNKGGIRL SFSKNPLGVR GSNSRSKSGY SFNGSYGKS
