ID   WHIA_BACSU              Reviewed;         316 AA.
AC   O06975; Q795F9;
DT   26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Probable cell division protein WhiA {ECO:0000255|HAMAP-Rule:MF_01420, ECO:0000305};
GN   Name=whiA {ECO:0000255|HAMAP-Rule:MF_01420, ECO:0000303|PubMed:24097947};
GN   Synonyms=yvcL; OrderedLocusNames=BSU34750;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Denizot F.;
RL   Submitted (APR-1997) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   FUNCTION, SUBCELLULAR LOCATION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=24097947; DOI=10.1128/jb.00507-13;
RA   Surdova K., Gamba P., Claessen D., Siersma T., Jonker M.J., Errington J.,
RA   Hamoen L.W.;
RT   "The conserved DNA-binding protein WhiA is involved in cell division in
RT   Bacillus subtilis.";
RL   J. Bacteriol. 195:5450-5460(2013).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=29378890; DOI=10.1128/jb.00633-17;
RA   Bohorquez L.C., Surdova K., Jonker M.J., Hamoen L.W.;
RT   "The conserved DNA binding protein WhiA influences chromosome segregation
RT   in Bacillus subtilis.";
RL   J. Bacteriol. 200:E00633-E00633(2018).
CC   -!- FUNCTION: Involved in cell division and chromosome segregation
CC       (PubMed:24097947, PubMed:29378890). May influence the activity of FtsZ
CC       (PubMed:24097947). Binds DNA, but does not seem to function as a
CC       transcription factor (PubMed:24097947). {ECO:0000269|PubMed:24097947,
CC       ECO:0000269|PubMed:29378890}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, nucleoid
CC       {ECO:0000269|PubMed:24097947}.
CC   -!- INDUCTION: Constitutively expressed throughout the growth phase.
CC       {ECO:0000269|PubMed:24097947}.
CC   -!- DISRUPTION PHENOTYPE: Mutant grows slower and cells are longer compared
CC       to the wild-type strain. Mutation has a relatively mild effect on the
CC       sporulation. Mutant is sensitive to reduced FtsZ concentrations.
CC       Deletion of the gene in a zapA mutant background results in very
CC       filamentous cells that are blocked in proper Z-ring formation
CC       (PubMed:24097947). Mutant shows increased internucleoid distances
CC       (PubMed:29378890). {ECO:0000269|PubMed:24097947,
CC       ECO:0000269|PubMed:29378890}.
CC   -!- SIMILARITY: Belongs to the WhiA family. {ECO:0000255|HAMAP-
CC       Rule:MF_01420}.
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DR   EMBL; Z94043; CAB08059.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB15480.1; -; Genomic_DNA.
DR   PIR; B70032; B70032.
DR   RefSeq; NP_391355.1; NC_000964.3.
DR   RefSeq; WP_003243775.1; NZ_JNCM01000033.1.
DR   AlphaFoldDB; O06975; -.
DR   SMR; O06975; -.
DR   STRING; 224308.BSU34750; -.
DR   PaxDb; O06975; -.
DR   PRIDE; O06975; -.
DR   DNASU; 936540; -.
DR   EnsemblBacteria; CAB15480; CAB15480; BSU_34750.
DR   GeneID; 936540; -.
DR   KEGG; bsu:BSU34750; -.
DR   PATRIC; fig|224308.179.peg.3763; -.
DR   eggNOG; COG1481; Bacteria.
DR   InParanoid; O06975; -.
DR   OMA; CDAEAAW; -.
DR   PhylomeDB; O06975; -.
DR   BioCyc; BSUB:BSU34750-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0009295; C:nucleoid; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-UniRule.
DR   GO; GO:0043937; P:regulation of sporulation; IBA:GO_Central.
DR   Gene3D; 3.10.28.10; -; 1.
DR   HAMAP; MF_01420; HTH_type_WhiA; 1.
DR   InterPro; IPR027434; Homing_endonucl.
DR   InterPro; IPR018478; Sporu_reg_WhiA_N_dom.
DR   InterPro; IPR003802; Sporulation_regulator_WhiA.
DR   InterPro; IPR023054; Sporulation_regulator_WhiA_C.
DR   InterPro; IPR039518; WhiA_LAGLIDADG_dom.
DR   PANTHER; PTHR37307; PTHR37307; 1.
DR   Pfam; PF02650; HTH_WhiA; 1.
DR   Pfam; PF14527; LAGLIDADG_WhiA; 1.
DR   Pfam; PF10298; WhiA_N; 1.
DR   SUPFAM; SSF55608; SSF55608; 1.
DR   TIGRFAMs; TIGR00647; DNA_bind_WhiA; 1.
PE   2: Evidence at transcript level;
KW   Cell cycle; Cell division; Cytoplasm; DNA-binding; Reference proteome.
FT   CHAIN           1..316
FT                   /note="Probable cell division protein WhiA"
FT                   /id="PRO_0000376442"
FT   DNA_BIND        275..309
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_01420"
SQ   SEQUENCE   316 AA;  36340 MW;  7ADD0705FED4DC61 CRC64;
     MSFASETKKE LTNLEVKDCC INAELSALIR MNGALSFTNR HLVLDVQTEN AAIARRIYTL
     LKKQYDVSVE LLVRKKMRLK KNNVYIVRFS ENAKAILEDL KILGENFVFE RSISKELVKK
     RCCKRSYMRG AFLAGGSVNN PETSSYHLEI FSLYKEHNDS LCDLLNEFQL NSKTLERKKG
     YITYLKEAEK ITEFLNVIGA HNSLLRFEDV RIVRDMRNSV NRLVNCETAN LNKTIGASLR
     QVENIKYIDE RIGLEALPEK LREIAQLRID YQEVTLKELG EMVASGKISK SGINHRLRKL
     DEIAEQLRTG QTVTLK