CAN12_HUMAN
ID CAN12_HUMAN Reviewed; 719 AA.
AC Q6ZSI9;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Calpain-12;
DE EC=3.4.22.-;
DE AltName: Full=Calcium-activated neutral proteinase 12;
DE Short=CANP 12;
GN Name=CAPN12;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Thalamus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
CC -!- FUNCTION: Calcium-regulated non-lysosomal thiol-protease.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the peptidase C2 family. {ECO:0000305}.
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DR EMBL; AK127398; BAC86959.1; -; mRNA.
DR CCDS; CCDS12519.1; -.
DR RefSeq; NP_653292.2; NM_144691.4.
DR AlphaFoldDB; Q6ZSI9; -.
DR SMR; Q6ZSI9; -.
DR BioGRID; 127107; 37.
DR IntAct; Q6ZSI9; 2.
DR STRING; 9606.ENSP00000331636; -.
DR MEROPS; C02.017; -.
DR iPTMnet; Q6ZSI9; -.
DR PhosphoSitePlus; Q6ZSI9; -.
DR BioMuta; CAPN12; -.
DR DMDM; 54035703; -.
DR EPD; Q6ZSI9; -.
DR MassIVE; Q6ZSI9; -.
DR MaxQB; Q6ZSI9; -.
DR PaxDb; Q6ZSI9; -.
DR PeptideAtlas; Q6ZSI9; -.
DR PRIDE; Q6ZSI9; -.
DR ProteomicsDB; 68220; -.
DR Antibodypedia; 48135; 105 antibodies from 23 providers.
DR DNASU; 147968; -.
DR Ensembl; ENST00000328867.9; ENSP00000331636.3; ENSG00000182472.9.
DR GeneID; 147968; -.
DR KEGG; hsa:147968; -.
DR MANE-Select; ENST00000328867.9; ENSP00000331636.3; NM_144691.4; NP_653292.2.
DR UCSC; uc002ojd.2; human.
DR CTD; 147968; -.
DR DisGeNET; 147968; -.
DR GeneCards; CAPN12; -.
DR HGNC; HGNC:13249; CAPN12.
DR HPA; ENSG00000182472; Group enriched (gallbladder, liver).
DR MIM; 608839; gene.
DR neXtProt; NX_Q6ZSI9; -.
DR OpenTargets; ENSG00000182472; -.
DR PharmGKB; PA134863447; -.
DR VEuPathDB; HostDB:ENSG00000182472; -.
DR eggNOG; KOG0045; Eukaryota.
DR GeneTree; ENSGT00940000161901; -.
DR HOGENOM; CLU_010982_0_2_1; -.
DR InParanoid; Q6ZSI9; -.
DR OMA; AKDVKWM; -.
DR OrthoDB; 704215at2759; -.
DR PhylomeDB; Q6ZSI9; -.
DR TreeFam; TF314748; -.
DR PathwayCommons; Q6ZSI9; -.
DR Reactome; R-HSA-1474228; Degradation of the extracellular matrix.
DR SignaLink; Q6ZSI9; -.
DR BioGRID-ORCS; 147968; 16 hits in 1076 CRISPR screens.
DR ChiTaRS; CAPN12; human.
DR GenomeRNAi; 147968; -.
DR Pharos; Q6ZSI9; Tdark.
DR PRO; PR:Q6ZSI9; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q6ZSI9; protein.
DR Bgee; ENSG00000182472; Expressed in pancreatic ductal cell and 140 other tissues.
DR ExpressionAtlas; Q6ZSI9; baseline and differential.
DR Genevisible; Q6ZSI9; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR CDD; cd00214; Calpain_III; 1.
DR CDD; cd00044; CysPc; 1.
DR InterPro; IPR033883; C2_III.
DR InterPro; IPR022684; Calpain_cysteine_protease.
DR InterPro; IPR022682; Calpain_domain_III.
DR InterPro; IPR022683; Calpain_III.
DR InterPro; IPR036213; Calpain_III_sf.
DR InterPro; IPR029545; CAPN12.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR000169; Pept_cys_AS.
DR InterPro; IPR001300; Peptidase_C2_calpain_cat.
DR PANTHER; PTHR10183:SF280; PTHR10183:SF280; 1.
DR Pfam; PF01067; Calpain_III; 1.
DR Pfam; PF00648; Peptidase_C2; 1.
DR PRINTS; PR00704; CALPAIN.
DR SMART; SM00720; calpain_III; 1.
DR SMART; SM00230; CysPc; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR SUPFAM; SSF49758; SSF49758; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
DR PROSITE; PS50203; CALPAIN_CAT; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
DR PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
PE 2: Evidence at transcript level;
KW Calcium; Hydrolase; Metal-binding; Protease; Reference proteome;
KW Thiol protease.
FT CHAIN 1..719
FT /note="Calpain-12"
FT /id="PRO_0000207730"
FT DOMAIN 45..341
FT /note="Calpain catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00239"
FT DOMAIN 620..655
FT /note="EF-hand"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT REGION 342..540
FT /note="Domain III"
FT REGION 393..418
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 541..719
FT /note="Domain IV"
FT ACT_SITE 105
FT /evidence="ECO:0000250"
FT ACT_SITE 259
FT /evidence="ECO:0000250"
FT ACT_SITE 283
FT /evidence="ECO:0000250"
FT BINDING 633
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 635
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 637
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 639
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT BINDING 644
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00448"
FT VARIANT 287
FT /note="C -> R (in dbSNP:rs12983010)"
FT /id="VAR_051516"
SQ SEQUENCE 719 AA; 81037 MW; BEACE2E5DD5839F2 CRC64;
MASSSGRVTI QLVDEEAGVG AGRLQLFRGQ SYEAIRAACL DSGILFRDPY FPAGPDALGY
DQLGPDSEKA KGVKWMRPHE FCAEPKFICE DMSRTDVCQG SLGNCWFLAA AASLTLYPRL
LRRVVPPGQD FQHGYAGVFH FQLWQFGRWM DVVVDDRLPV REGKLMFVRS EQRNEFWAPL
LEKAYAKLHG SYEVMRGGHM NEAFVDFTGG VGEVLYLRQN SMGLFSALRH ALAKESLVGA
TALSDRGEYR TEEGLVKGHA YSITGTHKVF LGFTKVRLLR LRNPWGCVEW TGAWSDSCPR
WDTLPTECRD ALLVKKEDGE FWMELRDFLL HFDTVQICSL SPEVLGPSPE GGGWHVHTFQ
GRWVRGFNSG GSQPNAETFW TNPQFRLTLL EPDEEDDEDE EGPWGGWGAA GARGPARGGR
TPKCTVLLSL IQRNRRRLRA KGLTYLTVGF HVFQIPEELL GLWDSPRSHA LLPRLLRADR
SPLSARRDVT RRCCLRPGHY LVVPSTAHAG DEADFTLRVF SERRHTAVEI DDVISADLQS
LQGPYLPLEL GLEQLFQELA GEEEELNASQ LQALLSIALE PARAHTSTPR EIGLRTCEQL
LQCFGHGQSL ALHHFQQLWG YLLEWQAIFN KFDEDTSGTM NSYELRLALN AAGFHLNNQL
TQTLTSRYRD SRLRVDFERF VSCVAHLTCI FCHCSQHLDG GEGVICLTHR QWMEVATFS
