CAN13_HUMAN
ID CAN13_HUMAN Reviewed; 669 AA.
AC Q6MZZ7; Q17RF0; Q580X1; Q8TE80;
DT 31-OCT-2006, integrated into UniProtKB/Swiss-Prot.
DT 31-OCT-2006, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Calpain-13;
DE EC=3.4.22.-;
DE AltName: Full=Calcium-activated neutral proteinase 13;
DE Short=CANP 13;
GN Name=CAPN13;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2), AND VARIANT THR-280.
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Esophageal carcinoma;
RX PubMed=17974005; DOI=10.1186/1471-2164-8-399;
RA Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U.,
RA Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D.,
RA Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A.,
RA Wiemann S., Schupp I.;
RT "The full-ORF clone resource of the German cDNA consortium.";
RL BMC Genomics 8:399-399(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15815621; DOI=10.1038/nature03466;
RA Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P.,
RA Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C.,
RA Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L.,
RA Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A.,
RA Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J.,
RA Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M.,
RA Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T.,
RA Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S.,
RA Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K.,
RA McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C.,
RA Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S.,
RA Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C.,
RA Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M.,
RA Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C.,
RA Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J.,
RA Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X.,
RA Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M.,
RA Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C.,
RA Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S.,
RA Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H.,
RA Wilson R.K.;
RT "Generation and annotation of the DNA sequences of human chromosomes 2 and
RT 4.";
RL Nature 434:724-731(2005).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT THR-280.
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=11675017; DOI=10.1016/s0378-1119(01)00599-6;
RA Dear T.N., Boehm T.;
RT "Identification and characterization of two novel calpain large subunit
RT genes.";
RL Gene 274:245-252(2001).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 515-669.
RG Structural genomics consortium (SGC);
RT "Structure of human calpain 13.";
RL Submitted (FEB-2009) to the PDB data bank.
CC -!- FUNCTION: Probable non-lysosomal thiol-protease. {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q6MZZ7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6MZZ7-2; Sequence=VSP_021476, VSP_021477;
CC -!- TISSUE SPECIFICITY: Weakly expressed in lung and testis. Weakly or not
CC expressed in other tissues. {ECO:0000269|PubMed:11675017}.
CC -!- SIMILARITY: Belongs to the peptidase C2 family. {ECO:0000305}.
CC -!- CAUTION: It is unlikely that this protein binds calcium as none of the
CC 2 EF-hand domains seem to contain a canonical calcium-binding site.
CC {ECO:0000305}.
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DR EMBL; AK074418; BAB85075.1; -; mRNA.
DR EMBL; BX640793; CAE45878.1; -; mRNA.
DR EMBL; AC092569; AAX82015.1; -; Genomic_DNA.
DR EMBL; BC117347; AAI17348.1; -; mRNA.
DR EMBL; BC117345; AAI17346.1; -; mRNA.
DR CCDS; CCDS46252.1; -. [Q6MZZ7-1]
DR RefSeq; NP_653176.2; NM_144575.2. [Q6MZZ7-1]
DR RefSeq; XP_011531461.1; XM_011533159.2. [Q6MZZ7-1]
DR RefSeq; XP_011531462.1; XM_011533160.2. [Q6MZZ7-1]
DR RefSeq; XP_011531463.1; XM_011533161.2. [Q6MZZ7-1]
DR RefSeq; XP_016860753.1; XM_017005264.1. [Q6MZZ7-1]
DR RefSeq; XP_016860754.1; XM_017005265.1.
DR PDB; 2I7A; X-ray; 1.80 A; A=515-669.
DR PDBsum; 2I7A; -.
DR AlphaFoldDB; Q6MZZ7; -.
DR SMR; Q6MZZ7; -.
DR BioGRID; 124928; 19.
DR IntAct; Q6MZZ7; 12.
DR STRING; 9606.ENSP00000295055; -.
DR MEROPS; C02.020; -.
DR iPTMnet; Q6MZZ7; -.
DR PhosphoSitePlus; Q6MZZ7; -.
DR BioMuta; CAPN13; -.
DR DMDM; 117949375; -.
DR jPOST; Q6MZZ7; -.
DR MassIVE; Q6MZZ7; -.
DR MaxQB; Q6MZZ7; -.
DR PaxDb; Q6MZZ7; -.
DR PeptideAtlas; Q6MZZ7; -.
DR PRIDE; Q6MZZ7; -.
DR ProteomicsDB; 66593; -. [Q6MZZ7-1]
DR ProteomicsDB; 66594; -. [Q6MZZ7-2]
DR Antibodypedia; 28998; 83 antibodies from 22 providers.
DR DNASU; 92291; -.
DR Ensembl; ENST00000295055.12; ENSP00000295055.8; ENSG00000162949.16. [Q6MZZ7-1]
DR Ensembl; ENST00000458085.6; ENSP00000416191.2; ENSG00000162949.16. [Q6MZZ7-2]
DR GeneID; 92291; -.
DR KEGG; hsa:92291; -.
DR MANE-Select; ENST00000295055.12; ENSP00000295055.8; NM_144575.3; NP_653176.2.
DR UCSC; uc061hvv.1; human. [Q6MZZ7-1]
DR CTD; 92291; -.
DR DisGeNET; 92291; -.
DR GeneCards; CAPN13; -.
DR HGNC; HGNC:16663; CAPN13.
DR HPA; ENSG00000162949; Tissue enhanced (intestine, stomach).
DR MIM; 610228; gene.
DR neXtProt; NX_Q6MZZ7; -.
DR OpenTargets; ENSG00000162949; -.
DR PharmGKB; PA134931170; -.
DR VEuPathDB; HostDB:ENSG00000162949; -.
DR eggNOG; KOG0037; Eukaryota.
DR eggNOG; KOG0045; Eukaryota.
DR GeneTree; ENSGT00940000160921; -.
DR HOGENOM; CLU_010982_0_3_1; -.
DR InParanoid; Q6MZZ7; -.
DR OMA; SQHAYTV; -.
DR OrthoDB; 704215at2759; -.
DR PhylomeDB; Q6MZZ7; -.
DR TreeFam; TF314748; -.
DR PathwayCommons; Q6MZZ7; -.
DR Reactome; R-HSA-1474228; Degradation of the extracellular matrix.
DR SignaLink; Q6MZZ7; -.
DR BioGRID-ORCS; 92291; 9 hits in 1067 CRISPR screens.
DR ChiTaRS; CAPN13; human.
DR EvolutionaryTrace; Q6MZZ7; -.
DR GenomeRNAi; 92291; -.
DR Pharos; Q6MZZ7; Tdark.
DR PRO; PR:Q6MZZ7; -.
DR Proteomes; UP000005640; Chromosome 2.
DR RNAct; Q6MZZ7; protein.
DR Bgee; ENSG00000162949; Expressed in palpebral conjunctiva and 125 other tissues.
DR ExpressionAtlas; Q6MZZ7; baseline and differential.
DR Genevisible; Q6MZZ7; HS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IBA:GO_Central.
DR GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR CDD; cd00044; CysPc; 1.
DR InterPro; IPR022684; Calpain_cysteine_protease.
DR InterPro; IPR022682; Calpain_domain_III.
DR InterPro; IPR036213; Calpain_III_sf.
DR InterPro; IPR029544; CANP13.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR001300; Peptidase_C2_calpain_cat.
DR PANTHER; PTHR10183:SF333; PTHR10183:SF333; 1.
DR Pfam; PF01067; Calpain_III; 1.
DR Pfam; PF00648; Peptidase_C2; 1.
DR PRINTS; PR00704; CALPAIN.
DR SMART; SM00230; CysPc; 1.
DR SUPFAM; SSF47473; SSF47473; 1.
DR SUPFAM; SSF49758; SSF49758; 1.
DR SUPFAM; SSF54001; SSF54001; 1.
DR PROSITE; PS50203; CALPAIN_CAT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Hydrolase; Protease;
KW Reference proteome; Repeat; Thiol protease.
FT CHAIN 1..669
FT /note="Calpain-13"
FT /id="PRO_0000259582"
FT DOMAIN 34..333
FT /note="Calpain catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00239"
FT DOMAIN 538..573
FT /note="EF-hand 1"
FT DOMAIN 636..669
FT /note="EF-hand 2"
FT ACT_SITE 93
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00239"
FT ACT_SITE 249
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00239"
FT ACT_SITE 273
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00239"
FT VAR_SEQ 417..423
FT /note="RFREKFP -> KHCPKLK (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_021476"
FT VAR_SEQ 424..669
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_021477"
FT VARIANT 249
FT /note="H -> Y (in dbSNP:rs17010210)"
FT /id="VAR_028964"
FT VARIANT 280
FT /note="A -> T (in dbSNP:rs508405)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334"
FT /id="VAR_028965"
FT VARIANT 596
FT /note="I -> T (in dbSNP:rs2276568)"
FT /id="VAR_028966"
FT CONFLICT 562
FT /note="E -> G (in Ref. 2; CAE45878)"
FT /evidence="ECO:0000305"
FT HELIX 518..529
FT /evidence="ECO:0007829|PDB:2I7A"
FT HELIX 540..550
FT /evidence="ECO:0007829|PDB:2I7A"
FT STRAND 555..558
FT /evidence="ECO:0007829|PDB:2I7A"
FT HELIX 560..580
FT /evidence="ECO:0007829|PDB:2I7A"
FT STRAND 586..588
FT /evidence="ECO:0007829|PDB:2I7A"
FT HELIX 589..591
FT /evidence="ECO:0007829|PDB:2I7A"
FT HELIX 592..597
FT /evidence="ECO:0007829|PDB:2I7A"
FT HELIX 600..602
FT /evidence="ECO:0007829|PDB:2I7A"
FT HELIX 609..619
FT /evidence="ECO:0007829|PDB:2I7A"
FT STRAND 624..626
FT /evidence="ECO:0007829|PDB:2I7A"
FT HELIX 628..648
FT /evidence="ECO:0007829|PDB:2I7A"
FT STRAND 650..653
FT /evidence="ECO:0007829|PDB:2I7A"
FT HELIX 659..667
FT /evidence="ECO:0007829|PDB:2I7A"
SQ SEQUENCE 669 AA; 76696 MW; BB24CF09D55CF790 CRC64;
MAYYQEPSVE TSIIKFKDQD FTTLRDHCLS MGRTFKDETF PAADSSIGQK LLQEKRLSNV
IWKRPQDLPG GPPHFILDDI SRFDIQQGGA ADCWFLAALG SLTQNPQYRQ KILMVQSFSH
QYAGIFRFRF WQCGQWVEVV IDDRLPVQGD KCLFVRPRHQ NQEFWPCLLE KAYAKLLGSY
SDLHYGFLED ALVDLTGGVI TNIHLHSSPV DLVKAVKTAT KAGSLITCAT PSGPTDTAQA
MENGLVSLHA YTVTGAEQIQ YRRGWEEIIS LWNPWGWGEA EWRGRWSDGS QEWEETCDPR
KSQLHKKRED GEFWMSCQDF QQKFIAMFIC SEIPITLDHG NTLHEGWSQI MFRKQVILGN
TAGGPRNDAQ FNFSVQEPME GTNVVVCVTV AVTPSNLKAE DAKFPLDFQV ILAGSQRFRE
KFPPVFFSSF RNTVQSSNNK FRRNFTMTYH LSPGNYVVVA QTRRKSAEFL LRIFLKMPDS
DRHLSSHFNL RMKGSPSEHG SQQSIFNRYA QQRLDIDATQ LQGLLNQELL TGPPGDMFSL
DECRSLVALM ELKVNGRLDQ EEFARLWKRL VHYQHVFQKV QTSPGVLLSS DLWKAIENTD
FLRGIFISRE LLHLVTLRYS DSVGRVSFPS LVCFLMRLEA MAKTFRNLSK DGKGLYLTEM
EWMSLVMYN
