CAN15_MOUSE
ID CAN15_MOUSE Reviewed; 1095 AA.
AC Q9JLG8; Q6PEE9;
DT 20-FEB-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Calpain-15;
DE EC=3.4.22.-;
DE AltName: Full=Small optic lobes homolog;
GN Name=Capn15; Synonyms=Solh;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RC STRAIN=BALB/cJ;
RX PubMed=10708520; DOI=10.1006/geno.1999.6098;
RA Kamei M., Webb G.C., Heydon K., Hendry I.A., Young I.G., Campbell H.D.;
RT "Solh, the mouse homologue of the Drosophila melanogaster small optic lobes
RT gene: organization, chromosomal mapping, and localization of gene product
RT to the olfactory bulb.";
RL Genomics 64:82-89(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Fetal brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP DISRUPTION PHENOTYPE, AND TISSUE SPECIFICITY.
RX PubMed=32885237; DOI=10.1093/hmg/ddaa198;
RA Zha C., Farah C.A., Holt R.J., Ceroni F., Al-Abdi L., Thuriot F.,
RA Khan A.O., Helaby R., Levesque S., Alkuraya F.S., Kraus A., Ragge N.K.,
RA Sossin W.S.;
RT "Biallelic variants in the small optic lobe calpain CAPN15 are associated
RT with congenital eye anomalies, deafness and other neurodevelopmental
RT deficits.";
RL Hum. Mol. Genet. 29:3054-3063(2020).
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9JLG8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9JLG8-2; Sequence=VSP_023363, VSP_023364, VSP_023365;
CC -!- TISSUE SPECIFICITY: Highly expressed in the brain and eye during
CC development (PubMed:32885237). Expressed in olfactory bulbs (at protein
CC level) (PubMed:10708520). {ECO:0000269|PubMed:10708520,
CC ECO:0000269|PubMed:32885237}.
CC -!- DISRUPTION PHENOTYPE: Knockout animals show significantly reduced
CC viability. Homozygous knockout mice also weigh significantly less than
CC their wild-type or heterozygous littermates. These mice also display
CC severe developmental eye defects, including anophthalmia,
CC microphthalmia, and cataract, and the presence of these phenotypes is
CC significantly increased compared to wild-type or heterozygous mice.
CC {ECO:0000269|PubMed:32885237}.
CC -!- SIMILARITY: Belongs to the peptidase C2 family. {ECO:0000305}.
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DR EMBL; AF180445; AAF62871.1; -; Genomic_DNA.
DR EMBL; BC058094; AAH58094.1; -; mRNA.
DR CCDS; CCDS28540.1; -. [Q9JLG8-1]
DR RefSeq; NP_056645.1; NM_015830.1. [Q9JLG8-1]
DR AlphaFoldDB; Q9JLG8; -.
DR SMR; Q9JLG8; -.
DR BioGRID; 206132; 2.
DR STRING; 10090.ENSMUSP00000039528; -.
DR iPTMnet; Q9JLG8; -.
DR PhosphoSitePlus; Q9JLG8; -.
DR MaxQB; Q9JLG8; -.
DR PaxDb; Q9JLG8; -.
DR PRIDE; Q9JLG8; -.
DR ProteomicsDB; 265522; -. [Q9JLG8-1]
DR ProteomicsDB; 265523; -. [Q9JLG8-2]
DR Antibodypedia; 22715; 76 antibodies from 15 providers.
DR DNASU; 50817; -.
DR Ensembl; ENSMUST00000041641; ENSMUSP00000039528; ENSMUSG00000037326. [Q9JLG8-1]
DR Ensembl; ENSMUST00000212149; ENSMUSP00000148718; ENSMUSG00000037326. [Q9JLG8-1]
DR GeneID; 50817; -.
DR KEGG; mmu:50817; -.
DR UCSC; uc008bcz.1; mouse. [Q9JLG8-1]
DR CTD; 6650; -.
DR MGI; MGI:1355075; Capn15.
DR VEuPathDB; HostDB:ENSMUSG00000037326; -.
DR eggNOG; KOG0045; Eukaryota.
DR GeneTree; ENSGT00940000158312; -.
DR HOGENOM; CLU_003001_0_0_1; -.
DR InParanoid; Q9JLG8; -.
DR OMA; HELMPHG; -.
DR PhylomeDB; Q9JLG8; -.
DR TreeFam; TF322245; -.
DR Reactome; R-MMU-1474228; Degradation of the extracellular matrix.
DR BioGRID-ORCS; 50817; 7 hits in 73 CRISPR screens.
DR ChiTaRS; Capn15; mouse.
DR PRO; PR:Q9JLG8; -.
DR Proteomes; UP000000589; Chromosome 17.
DR RNAct; Q9JLG8; protein.
DR Bgee; ENSMUSG00000037326; Expressed in motor neuron and 249 other tissues.
DR ExpressionAtlas; Q9JLG8; baseline and differential.
DR Genevisible; Q9JLG8; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IBA:GO_Central.
DR CDD; cd00044; CysPc; 1.
DR InterPro; IPR022684; Calpain_cysteine_protease.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR000169; Pept_cys_AS.
DR InterPro; IPR001300; Peptidase_C2_calpain_cat.
DR InterPro; IPR001876; Znf_RanBP2.
DR InterPro; IPR036443; Znf_RanBP2_sf.
DR Pfam; PF00648; Peptidase_C2; 1.
DR Pfam; PF00641; zf-RanBP; 4.
DR PRINTS; PR00704; CALPAIN.
DR SMART; SM00230; CysPc; 1.
DR SMART; SM00547; ZnF_RBZ; 5.
DR SUPFAM; SSF54001; SSF54001; 1.
DR SUPFAM; SSF90209; SSF90209; 4.
DR PROSITE; PS50203; CALPAIN_CAT; 1.
DR PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
DR PROSITE; PS01358; ZF_RANBP2_1; 5.
DR PROSITE; PS50199; ZF_RANBP2_2; 4.
PE 1: Evidence at protein level;
KW Alternative splicing; Hydrolase; Metal-binding; Phosphoprotein; Protease;
KW Reference proteome; Repeat; Thiol protease; Zinc; Zinc-finger.
FT CHAIN 1..1095
FT /note="Calpain-15"
FT /id="PRO_0000278771"
FT DOMAIN 497..803
FT /note="Calpain catalytic"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00239"
FT ZN_FING 3..32
FT /note="RanBP2-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00322"
FT ZN_FING 44..73
FT /note="RanBP2-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00322"
FT ZN_FING 148..178
FT /note="RanBP2-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00322"
FT ZN_FING 348..379
FT /note="RanBP2-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00322"
FT ZN_FING 422..451
FT /note="RanBP2-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00322"
FT REGION 96..153
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 204..292
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1074..1095
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 217..234
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 267..283
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 562
FT /evidence="ECO:0000250"
FT ACT_SITE 727
FT /evidence="ECO:0000250"
FT ACT_SITE 747
FT /evidence="ECO:0000250"
FT MOD_RES 311
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75808"
FT MOD_RES 345
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75808"
FT MOD_RES 348
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75808"
FT MOD_RES 1079
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O75808"
FT VAR_SEQ 921
FT /note="Q -> QGKGPSQMRPTPSFPTSEPQSPPTGKLLAPLSTPPT (in
FT isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023363"
FT VAR_SEQ 978..1037
FT /note="GREGMTCYYLTHGWAGLIVVVENRHPKSYLHVQCDCTDSFNVVSTRGSLRTQ
FT DSVPPLHR -> VGGVLMGEGRGWATTSPGPPTLCLCPGSRGHDLLLPDSWLGGTHRGS
FT GEPAPQVLPACAM (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023364"
FT VAR_SEQ 1038..1095
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_023365"
SQ SEQUENCE 1095 AA; 118719 MW; 081E1DFED2AE5D28 CRC64;
MATVGEWSCA RCTFLNPAGQ RQCSICEAPR HKPDLDQILR LSVEEQKWPC ARCTFRNFLG
KEACEVCGFT PEPVPGAPLL PIINGVLPKP PTILVEPKGS GKEEAGPVRT AGLVATEPAR
GRPEGEEERE ERGEEEEKEQ EGEGERAEPG SGWACQRCTL HNTPVASSCS ACGGPRKLSL
PRIPPEALVV PEVVAPTGFH VVPAPSQPVL PGEGAEADSP STSQGPTSTD QEPPRVPLFS
PFSPTLQNNP VPRSRREVPP QLQPPVPEAV QSSASTSSKG PQQGPGRAAA GASRLAELLS
GKELLPGKRL SVLEEEVPES SPARCESCSD VIDLAGDIVR YTPASPSSPD FTTWSCARCT
LRNPTTAPRC SVCGGSKLHG FQEHSEPPTH CPDCGANKPG PCVGSCGRAP SAHKAVRLLP
DRPGQWACPA CTLINTPRAK HCAACHTPQL LVTQCRGATP LRRRESMHVE KRRQTDEGEA
KALWENIVAF CRENSVNFVD DSFPPGPASV GFPVGDSVQQ RVKQWLRPHE INCSVFRDHG
TPWSVFHTLR PSDILQGLLG NCWFLSALAV LAERPDLVER VMVTRSLCAE GAYQVRLCKD
GTWTTVLVDD MLPCDEAGFL LFSQAQRKQL WVALIEKALA KLHGSYFALQ AGRAIEGLAT
LTGAPCESLA LQVSSTNPRE EPVDTDLIWA KMLSSKEAGF LMGASCGGGN MKVDDAAYES
LGLRPRHAYS VLDVRDVQGS RLLRLRNPWG RFSWNGSWSD EWPHWPGHLR AELMPHGSSE
GVFWMEYSDF IRYFDSVDIC KVHSDWQEAR VQGCFPSTAG GPVGVTALTV LERASLEFAL
FQEGSRRSDS VDSHLLDLCI LVFRATFGTG GRLSLGRLLA HSKRAVKKFV NCDVMLEPGE
YAVVCCAFNH WNPAPPGPPA QASSPSAGVP RGAPEPPGHV LAVYSSRLVM VEPVEAQPTT
LADAIILLTE SRGERHEGRE GMTCYYLTHG WAGLIVVVEN RHPKSYLHVQ CDCTDSFNVV
STRGSLRTQD SVPPLHRQVL VILSQLEGNA GFSITHRLAH RKAAQAFLSD WTASRGTHSP
PLTPDVAGLH GPRPL
