WHIA_STRCO
ID WHIA_STRCO Reviewed; 328 AA.
AC Q9Z515; Q9S4Y1;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 25-MAY-2022, entry version 101.
DE RecName: Full=Probable cell division protein WhiA {ECO:0000255|HAMAP-Rule:MF_01420, ECO:0000305};
GN Name=whiA {ECO:0000255|HAMAP-Rule:MF_01420}; OrderedLocusNames=SCO1950;
GN ORFNames=SCC54.10c;
OS Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Streptomyces; Streptomyces albidoflavus group.
OX NCBI_TaxID=100226;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], MUTAGENESIS OF LEU-160 AND LEU-194,
RP DEVELOPMENTAL STAGE, POSSIBLE OPERON STRUCTURE, DISRUPTION PHENOTYPE, AND
RP INDUCTION.
RC STRAIN=ATCC BAA-471 / A3(2) / M145;
RX PubMed=10986251; DOI=10.1128/jb.182.19.5470-5478.2000;
RA Ainsa J.A., Ryding N.J., Hartley N., Findlay K.C., Bruton C.J.,
RA Chater K.F.;
RT "WhiA, a protein of unknown function conserved among gram-positive
RT bacteria, is essential for sporulation in Streptomyces coelicolor A3(2).";
RL J. Bacteriol. 182:5470-5478(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC BAA-471 / A3(2) / M145;
RX PubMed=12000953; DOI=10.1038/417141a;
RA Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT A3(2).";
RL Nature 417:141-147(2002).
RN [3]
RP FUNCTION IN PARB EXPRESSION, AND REGULATION MODEL.
RC STRAIN=ATCC BAA-471 / A3(2) / M145;
RX PubMed=16484182; DOI=10.1128/jb.188.5.1710-1720.2006;
RA Jakimowicz D., Mouz S., Zakrzewska-Czerwinska J., Chater K.F.;
RT "Developmental control of a parAB promoter leads to formation of
RT sporulation-associated ParB complexes in Streptomyces coelicolor.";
RL J. Bacteriol. 188:1710-1720(2006).
RN [4]
RP SUGGESTION OF DNA-BINDING.
RX PubMed=17603302; DOI=10.4161/cc.6.13.4471;
RA Knizewski L., Ginalski K.;
RT "Bacterial DUF199/COG1481 proteins including sporulation regulator WhiA are
RT distant homologs of LAGLIDADG homing endonucleases that retained only DNA
RT binding.";
RL Cell Cycle 6:1666-1670(2007).
RN [5]
RP REVIEW.
RX PubMed=16911038; DOI=10.1111/j.1574-6976.2006.00033.x;
RA Chater K.F., Chandra G.;
RT "The evolution of development in Streptomyces analysed by genome
RT comparisons.";
RL FEMS Microbiol. Rev. 30:651-672(2006).
RN [6]
RP FUNCTION, DNA-BINDING, SUBUNIT, INDUCTION, AND DOMAIN.
RX PubMed=22355671; DOI=10.1038/srep00156;
RA Kaiser B.K., Stoddard B.L.;
RT "DNA recognition and transcriptional regulation by the WhiA sporulation
RT factor.";
RL Sci. Rep. 1:156-156(2011).
CC -!- FUNCTION: Involved in cell division and chromosome segregation (By
CC similarity). Involved in sporulation (PubMed:16484182,
CC PubMed:22355671). May coordinate the cessation of aerial hyphae growth
CC and subsequent chromosome segregation and/or septation
CC (PubMed:16484182, PubMed:22355671). Required for expression of the ParB
CC partioning protein during sporogenesis. Activates its own transcription
CC and represses WhiB (PubMed:16484182). Binds with low affinity to its
CC own promoter and to the Parp2 sporulation-specific promoter. Also binds
CC directly to the RNA polymerase sigma factor WhiG, leading to inhibition
CC of WhiG-dependent transcription in a dose-dependent manner
CC (PubMed:22355671). {ECO:0000255|HAMAP-Rule:MF_01420,
CC ECO:0000269|PubMed:16484182, ECO:0000269|PubMed:22355671}.
CC -!- SUBUNIT: Monomer in solution. {ECO:0000269|PubMed:22355671}.
CC -!- DEVELOPMENTAL STAGE: Induced when aerial mycelia start forming,
CC expression is maximal when sporulation starts.
CC {ECO:0000269|PubMed:10986251}.
CC -!- INDUCTION: Transcribed both as part of an operon and from its own
CC specific promoter during sporulation (PubMed:10986251). Transcription
CC is stimulated by WhiG (PubMed:22355671). {ECO:0000269|PubMed:10986251,
CC ECO:0000269|PubMed:22355671}.
CC -!- DOMAIN: DNA binding is primarily driven by the H-T-H domain, but
CC requires full-length protein for maximum affinity.
CC {ECO:0000269|PubMed:22355671}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene do not sporulate; instead
CC of aerial hyphae of unicellular spores, long slightly coiled unseptated
CC stalks form. {ECO:0000269|PubMed:10986251}.
CC -!- SIMILARITY: Belongs to the WhiA family. {ECO:0000255|HAMAP-
CC Rule:MF_01420}.
CC -!- CAUTION: There may be second mutations present in each of mutant
CC strains C58 and C213. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB38140.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF106003; AAD42169.1; -; Genomic_DNA.
DR EMBL; AL939110; CAB38140.1; ALT_INIT; Genomic_DNA.
DR PIR; T36023; T36023.
DR RefSeq; NP_626214.1; NC_003888.3.
DR RefSeq; WP_011028060.1; NC_003888.3.
DR AlphaFoldDB; Q9Z515; -.
DR SMR; Q9Z515; -.
DR STRING; 100226.SCO1950; -.
DR PRIDE; Q9Z515; -.
DR GeneID; 1097384; -.
DR KEGG; sco:SCO1950; -.
DR PATRIC; fig|100226.15.peg.1976; -.
DR eggNOG; COG1481; Bacteria.
DR HOGENOM; CLU_053282_0_0_11; -.
DR InParanoid; Q9Z515; -.
DR OMA; CDAEAAW; -.
DR PhylomeDB; Q9Z515; -.
DR Proteomes; UP000001973; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-UniRule.
DR GO; GO:0043937; P:regulation of sporulation; IMP:UniProtKB.
DR Gene3D; 3.10.28.10; -; 1.
DR HAMAP; MF_01420; HTH_type_WhiA; 1.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR018478; Sporu_reg_WhiA_N_dom.
DR InterPro; IPR003802; Sporulation_regulator_WhiA.
DR InterPro; IPR023054; Sporulation_regulator_WhiA_C.
DR InterPro; IPR039518; WhiA_LAGLIDADG_dom.
DR PANTHER; PTHR37307; PTHR37307; 1.
DR Pfam; PF02650; HTH_WhiA; 1.
DR Pfam; PF14527; LAGLIDADG_WhiA; 1.
DR Pfam; PF10298; WhiA_N; 1.
DR TIGRFAMs; TIGR00647; DNA_bind_WhiA; 1.
PE 1: Evidence at protein level;
KW Activator; Cell cycle; Cell division; DNA-binding; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..328
FT /note="Probable cell division protein WhiA"
FT /id="PRO_0000376600"
FT DNA_BIND 273..306
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01420"
FT MUTAGEN 160
FT /note="L->P: Aerial hyphae have coiled segments and no
FT signs of septation (strain C85)."
FT /evidence="ECO:0000269|PubMed:10986251"
FT MUTAGEN 194
FT /note="L->P: Aerial hyphae have coiled segments and no
FT signs of septation (strain C213)."
FT /evidence="ECO:0000269|PubMed:10986251"
SQ SEQUENCE 328 AA; 34738 MW; 6C7E48744A537947 CRC64;
MTAAVKSEIS QLPVTRTCCR KAEVSAVLRF AGGLHLVSGR IVIEAELDTG NAARRLKRDI
LEIFGHSSEL IVMAPGGLRR GSRFVVRVVA GGDQLARQTG LVDGRGRPIR GLPPQVVSGA
TCDAEAAWRG AFLAHGSLTE PGRSSSLEVT CPGPEAALAL VGAARRLSIP AKAREVRGVD
RVVVRDGDAI GALLTRLGAH DSVLAWEERR LRREVRATAN RLANFDDANL RRSARAAVAA
GARVQRALEI LADDVPEHLA AAGRLRMEHK QASLEELGAL ADPPLTKDAV AGRIRRLLAM
ADKRASDLGI AGTDANLGEE ELADNLVG
