WHIA_STRVP
ID WHIA_STRVP Reviewed; 327 AA.
AC F2RGQ4;
DT 12-SEP-2018, integrated into UniProtKB/Swiss-Prot.
DT 31-MAY-2011, sequence version 1.
DT 25-MAY-2022, entry version 52.
DE RecName: Full=Probable cell division protein WhiA {ECO:0000255|HAMAP-Rule:MF_01420, ECO:0000305};
GN Name=whiA {ECO:0000255|HAMAP-Rule:MF_01420, ECO:0000303|PubMed:24065632};
GN OrderedLocusNames=SVEN_1578 {ECO:0000312|EMBL:CCA54865.1};
OS Streptomyces venezuelae (strain ATCC 10712 / CBS 650.69 / DSM 40230 / JCM
OS 4526 / NBRC 13096 / PD 04745).
OC Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC Streptomyces.
OX NCBI_TaxID=953739;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD
RC 04745;
RX PubMed=21463507; DOI=10.1186/1471-2164-12-175;
RA Pullan S.T., Chandra G., Bibb M.J., Merrick M.;
RT "Genome-wide analysis of the role of GlnR in Streptomyces venezuelae
RT provides new insights into global nitrogen regulation in actinomycetes.";
RL BMC Genomics 12:175-175(2011).
RN [2]
RP FUNCTION, DNA-BINDING, INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD
RC 04745;
RX PubMed=24065632; DOI=10.1128/mbio.00684-13;
RA Bush M.J., Bibb M.J., Chandra G., Findlay K.C., Buttner M.J.;
RT "Genes required for aerial growth, cell division, and chromosome
RT segregation are targets of WhiA before sporulation in Streptomyces
RT venezuelae.";
RL MBio 4:E00684-E00684(2013).
RN [3]
RP FUNCTION, AND ACTIVITY REGULATION.
RC STRAIN=ATCC 10712 / CBS 650.69 / DSM 40230 / JCM 4526 / NBRC 13096 / PD
RC 04745;
RX PubMed=27094333; DOI=10.1128/mbio.00523-16;
RA Bush M.J., Chandra G., Bibb M.J., Findlay K.C., Buttner M.J.;
RT "Genome-wide chromatin immunoprecipitation sequencing analysis shows that
RT WhiB is a transcription factor that cocontrols its regulon with WhiA to
RT initiate developmental cell division in Streptomyces.";
RL MBio 7:E00523-E00523(2016).
CC -!- FUNCTION: Involved in cell division and chromosome segregation
CC (PubMed:24065632). Plays a central role in the initiation of
CC sporulation through the activation of multiple components of the cell
CC division machinery (PubMed:24065632). Acts as a transcriptional
CC regulator that is involved in the direct control of genes required for
CC key steps in sporulation, including the cessation of aerial growth, the
CC initiation of cell division, and chromosome segregation. Functions
CC almost equally as an activator and as a repressor (PubMed:24065632).
CC Required for DNA binding by WhiB. May function cooperatively with WhiB
CC (PubMed:27094333). {ECO:0000269|PubMed:24065632,
CC ECO:0000269|PubMed:27094333}.
CC -!- ACTIVITY REGULATION: DNA binding depends on WhiB.
CC {ECO:0000269|PubMed:27094333}.
CC -!- INDUCTION: Autoregulated. Expressed throughout vegetative growth and
CC sporulation. Could be post-translationally regulated.
CC {ECO:0000269|PubMed:24065632}.
CC -!- DISRUPTION PHENOTYPE: Mutants form unusually long aerial hyphae that
CC fail to initiate septation or chromosome segregation.
CC {ECO:0000269|PubMed:24065632}.
CC -!- SIMILARITY: Belongs to the WhiA family. {ECO:0000255|HAMAP-
CC Rule:MF_01420}.
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DR EMBL; FR845719; CCA54865.1; -; Genomic_DNA.
DR AlphaFoldDB; F2RGQ4; -.
DR SMR; F2RGQ4; -.
DR STRING; 953739.SVEN_1578; -.
DR EnsemblBacteria; CCA54865; CCA54865; SVEN_1578.
DR KEGG; sve:SVEN_1578; -.
DR PATRIC; fig|953739.5.peg.3689; -.
DR eggNOG; COG1481; Bacteria.
DR HOGENOM; CLU_053282_0_0_11; -.
DR OMA; CDAEAAW; -.
DR Proteomes; UP000006854; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-UniRule.
DR GO; GO:0043937; P:regulation of sporulation; IEA:InterPro.
DR Gene3D; 3.10.28.10; -; 1.
DR HAMAP; MF_01420; HTH_type_WhiA; 1.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR018478; Sporu_reg_WhiA_N_dom.
DR InterPro; IPR003802; Sporulation_regulator_WhiA.
DR InterPro; IPR023054; Sporulation_regulator_WhiA_C.
DR InterPro; IPR039518; WhiA_LAGLIDADG_dom.
DR PANTHER; PTHR37307; PTHR37307; 1.
DR Pfam; PF02650; HTH_WhiA; 1.
DR Pfam; PF14527; LAGLIDADG_WhiA; 1.
DR Pfam; PF10298; WhiA_N; 1.
DR TIGRFAMs; TIGR00647; DNA_bind_WhiA; 1.
PE 1: Evidence at protein level;
KW Activator; Cell cycle; Cell division; DNA-binding; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..327
FT /note="Probable cell division protein WhiA"
FT /id="PRO_0000445016"
FT DNA_BIND 273..306
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01420"
FT REGION 308..327
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 327 AA; 34904 MW; 2296DD29432A0458 CRC64;
MTAAVKDEIS RLPVTRTCCR KAEVSSILRF AGGLHLVSGR IVIEAELDTA MAARRLKRDI
LEIFGHSSEL IVMAPGGLRR GSRFVVRVVA GGDQLARQTG LVDGRGRPIR GLPPQVVSGA
TCDAEAAWRG AFLAHGSLTE PGRSSSLEVT CPGPEAALAL VGAARRLSIA AKAREVRGVD
RVVVRDGDAI GALLTRLGAH ESVLAWEERR MRREVRATAN RLANFDDANL RRSARAAVAA
GARVQRALEI LGEEVPEHLA AAGRLRMEHK QASLEELGAL ADPPLTKDAV AGRIRRLLAM
ADKRAQDLGI PGTESNLTEE LDDSLVG