WHIA_THEMA
ID WHIA_THEMA Reviewed; 295 AA.
AC Q9X234;
DT 12-SEP-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 25-MAY-2022, entry version 118.
DE RecName: Full=Probable cell division protein WhiA {ECO:0000255|HAMAP-Rule:MF_01420, ECO:0000305};
GN Name=whiA {ECO:0000255|HAMAP-Rule:MF_01420, ECO:0000303|PubMed:19836336};
GN OrderedLocusNames=TM_1708 {ECO:0000312|EMBL:AAD36775.1};
OS Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826
OS / MSB8).
OC Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
OX NCBI_TaxID=243274;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8;
RX PubMed=10360571; DOI=10.1038/20601;
RA Nelson K.E., Clayton R.A., Gill S.R., Gwinn M.L., Dodson R.J., Haft D.H.,
RA Hickey E.K., Peterson J.D., Nelson W.C., Ketchum K.A., McDonald L.A.,
RA Utterback T.R., Malek J.A., Linher K.D., Garrett M.M., Stewart A.M.,
RA Cotton M.D., Pratt M.S., Phillips C.A., Richardson D.L., Heidelberg J.F.,
RA Sutton G.G., Fleischmann R.D., Eisen J.A., White O., Salzberg S.L.,
RA Smith H.O., Venter J.C., Fraser C.M.;
RT "Evidence for lateral gene transfer between Archaea and Bacteria from
RT genome sequence of Thermotoga maritima.";
RL Nature 399:323-329(1999).
RN [2] {ECO:0007744|PDB:3HYI, ECO:0007744|PDB:3HYJ}
RP X-RAY CRYSTALLOGRAPHY (2.34 ANGSTROMS), AND DOMAIN.
RX PubMed=19836336; DOI=10.1016/j.str.2009.08.008;
RA Kaiser B.K., Clifton M.C., Shen B.W., Stoddard B.L.;
RT "The structure of a bacterial DUF199/WhiA protein: domestication of an
RT invasive endonuclease.";
RL Structure 17:1368-1376(2009).
CC -!- FUNCTION: Involved in cell division and chromosome segregation.
CC {ECO:0000255|HAMAP-Rule:MF_01420}.
CC -!- DOMAIN: Contains a degenerated N-terminal LAGLIDADG homing endonuclease
CC (LHE) domain, a linker region and a C-terminal helix-turn-helix (HTH)
CC domain. {ECO:0000269|PubMed:19836336}.
CC -!- SIMILARITY: Belongs to the WhiA family. {ECO:0000255|HAMAP-
CC Rule:MF_01420}.
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DR EMBL; AE000512; AAD36775.1; -; Genomic_DNA.
DR PIR; F72221; F72221.
DR RefSeq; NP_229508.1; NC_000853.1.
DR PDB; 3HYI; X-ray; 2.34 A; A=1-295.
DR PDB; 3HYJ; X-ray; 2.60 A; A/D=1-198.
DR PDBsum; 3HYI; -.
DR PDBsum; 3HYJ; -.
DR AlphaFoldDB; Q9X234; -.
DR SMR; Q9X234; -.
DR STRING; 243274.THEMA_05700; -.
DR EnsemblBacteria; AAD36775; AAD36775; TM_1708.
DR KEGG; tma:TM1708; -.
DR PATRIC; fig|243274.18.peg.1101; -.
DR InParanoid; Q9X234; -.
DR OMA; RVQIFIP; -.
DR EvolutionaryTrace; Q9X234; -.
DR Proteomes; UP000008183; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-UniRule.
DR GO; GO:0043937; P:regulation of sporulation; IBA:GO_Central.
DR Gene3D; 3.10.28.10; -; 1.
DR HAMAP; MF_01420; HTH_type_WhiA; 1.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR003802; Sporulation_regulator_WhiA.
DR InterPro; IPR023054; Sporulation_regulator_WhiA_C.
DR InterPro; IPR039518; WhiA_LAGLIDADG_dom.
DR PANTHER; PTHR37307; PTHR37307; 1.
DR Pfam; PF02650; HTH_WhiA; 1.
DR Pfam; PF14527; LAGLIDADG_WhiA; 1.
DR SUPFAM; SSF55608; SSF55608; 1.
DR TIGRFAMs; TIGR00647; DNA_bind_WhiA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell cycle; Cell division; DNA-binding; Reference proteome.
FT CHAIN 1..295
FT /note="Probable cell division protein WhiA"
FT /id="PRO_0000445017"
FT DNA_BIND 262..293
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01420"
FT HELIX 9..16
FT /evidence="ECO:0007829|PDB:3HYI"
FT TURN 17..19
FT /evidence="ECO:0007829|PDB:3HYI"
FT HELIX 25..39
FT /evidence="ECO:0007829|PDB:3HYI"
FT STRAND 40..43
FT /evidence="ECO:0007829|PDB:3HYI"
FT TURN 44..47
FT /evidence="ECO:0007829|PDB:3HYI"
FT STRAND 48..54
FT /evidence="ECO:0007829|PDB:3HYI"
FT HELIX 56..68
FT /evidence="ECO:0007829|PDB:3HYI"
FT STRAND 73..79
FT /evidence="ECO:0007829|PDB:3HYI"
FT STRAND 83..86
FT /evidence="ECO:0007829|PDB:3HYJ"
FT STRAND 88..94
FT /evidence="ECO:0007829|PDB:3HYI"
FT HELIX 97..100
FT /evidence="ECO:0007829|PDB:3HYI"
FT STRAND 105..107
FT /evidence="ECO:0007829|PDB:3HYJ"
FT HELIX 108..122
FT /evidence="ECO:0007829|PDB:3HYI"
FT STRAND 123..125
FT /evidence="ECO:0007829|PDB:3HYI"
FT TURN 128..130
FT /evidence="ECO:0007829|PDB:3HYI"
FT STRAND 134..139
FT /evidence="ECO:0007829|PDB:3HYI"
FT HELIX 141..155
FT /evidence="ECO:0007829|PDB:3HYI"
FT STRAND 160..163
FT /evidence="ECO:0007829|PDB:3HYI"
FT STRAND 168..172
FT /evidence="ECO:0007829|PDB:3HYI"
FT HELIX 175..184
FT /evidence="ECO:0007829|PDB:3HYI"
FT HELIX 189..201
FT /evidence="ECO:0007829|PDB:3HYI"
FT HELIX 207..238
FT /evidence="ECO:0007829|PDB:3HYI"
FT HELIX 241..243
FT /evidence="ECO:0007829|PDB:3HYI"
FT HELIX 246..257
FT /evidence="ECO:0007829|PDB:3HYI"
FT HELIX 263..268
FT /evidence="ECO:0007829|PDB:3HYI"
FT TURN 269..271
FT /evidence="ECO:0007829|PDB:3HYI"
FT HELIX 274..288
FT /evidence="ECO:0007829|PDB:3HYI"
FT TURN 289..291
FT /evidence="ECO:0007829|PDB:3HYI"
SQ SEQUENCE 295 AA; 34244 MW; 442A432806A54DC8 CRC64;
MVSLLRRTFS EEIKEELVNV PFGSREEVIS ELLGFIKARG DLDVKSRHIV FSLHSFAASR
RLLNLMKYLS KPVSEIIVEK SHNIKKRYIK ITAEYSESFM VIEPFFDVAL FVSFLRGLFL
SGGSMTNPRY HYHLEINLFE EETLALTRKS LKDFFNINAG IIELRNTRKL YIKSIKDILV
FLEAIGVQRK LEEIDRIVTE RKVIGDVNRT VNFIEANAIR TANSTARQIR AIELIKENMG
LENLPEDLRR VALVRLRNKE LSLRELGKKL NLTKSQIYSK LKRIIKIAER FGDVK
