WHIB1_MYCTO
ID WHIB1_MYCTO Reviewed; 84 AA.
AC P9WF42; F2GK92; L0TEP0; O05847; Q7D5W8;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 38.
DE RecName: Full=Transcriptional regulator WhiB1;
GN Name=whiB1; OrderedLocusNames=MT3315;
OS Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83331;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CDC 1551 / Oshkosh;
RX PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT laboratory strains.";
RL J. Bacteriol. 184:5479-5490(2002).
RN [2]
RP INDUCTION.
RC STRAIN=CDC 1551 / Oshkosh;
RX PubMed=16870781; DOI=10.1128/aac.00295-06;
RA Geiman D.E., Raghunand T.R., Agarwal N., Bishai W.R.;
RT "Differential gene expression in response to exposure to antimycobacterial
RT agents and other stress conditions among seven Mycobacterium tuberculosis
RT whiB-like genes.";
RL Antimicrob. Agents Chemother. 50:2836-2841(2006).
RN [3]
RP INDUCTION.
RC STRAIN=CDC 1551 / Oshkosh;
RX PubMed=16946269; DOI=10.1099/mic.0.28924-0;
RA Agarwal N., Raghunand T.R., Bishai W.R.;
RT "Regulation of the expression of whiB1 in Mycobacterium tuberculosis: role
RT of cAMP receptor protein.";
RL Microbiology 152:2749-2756(2006).
RN [4]
RP INDUCTION.
RC STRAIN=CDC 1551 / Oshkosh;
RX PubMed=22829866; DOI=10.1371/journal.pone.0037516;
RA Larsson C., Luna B., Ammerman N.C., Maiga M., Agarwal N., Bishai W.R.;
RT "Gene expression of Mycobacterium tuberculosis putative transcription
RT factors whiB1-7 in redox environments.";
RL PLoS ONE 7:E37516-E37516(2012).
CC -!- FUNCTION: Acts as a transcriptional regulator. Probably redox-
CC responsive. The apo- but not holo-form probably binds DNA (By
CC similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC Note=Binds 1 [4Fe-4S] cluster per subunit. Following nitrosylation of
CC the [4Fe-4S] cluster binds 1 [4Fe-8(NO)] cluster per subunit.
CC {ECO:0000250};
CC -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- INDUCTION: Activated by CRP. Essentially constitutive over all growth
CC phases. 2-fold induced by ethanol, repressed by SDS and heat shock. Not
CC induced by hypoxia, slightly induced by NO and in macrophage and mouse
CC infection, 10-fold induced by cAMP. There are 2 CRP-binding sites in
CC the promoter of whiB1, at low concentrations of CRP with or without
CC cAMP transcription of whiB1 is enhanced via site CRP1, then repressed
CC as site CRP2 is filled. {ECO:0000269|PubMed:16870781,
CC ECO:0000269|PubMed:16946269, ECO:0000269|PubMed:22829866}.
CC -!- PTM: The Fe-S cluster can be nitrosylated by nitric oxide (NO).
CC {ECO:0000250}.
CC -!- PTM: Upon Fe-S cluster removal intramolecular disulfide bonds are
CC formed. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the WhiB family. {ECO:0000305}.
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DR EMBL; AE000516; AAK47657.1; -; Genomic_DNA.
DR PIR; D70596; D70596.
DR RefSeq; WP_003416884.1; NZ_KK341227.1.
DR AlphaFoldDB; P9WF42; -.
DR SMR; P9WF42; -.
DR EnsemblBacteria; AAK47657; AAK47657; MT3315.
DR GeneID; 45427212; -.
DR KEGG; mtc:MT3315; -.
DR PATRIC; fig|83331.31.peg.3570; -.
DR HOGENOM; CLU_106245_6_2_11; -.
DR Proteomes; UP000001020; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0035731; F:dinitrosyl-iron complex binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01479; WhiB; 1.
DR InterPro; IPR034768; 4FE4S_WBL.
DR InterPro; IPR003482; Whib.
DR PANTHER; PTHR38839; PTHR38839; 1.
DR Pfam; PF02467; Whib; 1.
DR PROSITE; PS51674; 4FE4S_WBL; 1.
PE 2: Evidence at transcript level;
KW 4Fe-4S; Cytoplasm; Disulfide bond; DNA-binding; Iron; Iron-sulfur;
KW Metal-binding; Transcription; Transcription regulation.
FT CHAIN 1..84
FT /note="Transcriptional regulator WhiB1"
FT /id="PRO_0000428604"
FT DOMAIN 8..70
FT /note="4Fe-4S Wbl-type"
FT BINDING 9
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 37
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 40
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 46
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
SQ SEQUENCE 84 AA; 9319 MW; E06C6D18F7C31ED2 CRC64;
MDWRHKAVCR DEDPELFFPV GNSGPALAQI ADAKLVCNRC PVTTECLSWA LNTGQDSGVW
GGMSEDERRA LKRRNARTKA RTGV
