ID   WHIB2_BIFLO             Reviewed;          99 AA.
AC   Q8G5K1;
DT   28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Transcriptional regulator WhiB2;
GN   Name=whiB2; OrderedLocusNames=BL1008;
OS   Bifidobacterium longum (strain NCC 2705).
OC   Bacteria; Actinobacteria; Bifidobacteriales; Bifidobacteriaceae;
OC   Bifidobacterium.
OX   NCBI_TaxID=206672;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCC 2705;
RX   PubMed=12381787; DOI=10.1073/pnas.212527599;
RA   Schell M.A., Karmirantzou M., Snel B., Vilanova D., Berger B., Pessi G.,
RA   Zwahlen M.-C., Desiere F., Bork P., Delley M., Pridmore R.D., Arigoni F.;
RT   "The genome sequence of Bifidobacterium longum reflects its adaptation to
RT   the human gastrointestinal tract.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:14422-14427(2002).
RN   [2]
RP   INDUCTION.
RC   STRAIN=B 397M;
RX   PubMed=22609519; DOI=10.1016/j.anaerobe.2012.04.011;
RA   Averina O.V., Zakharevich N.V., Danilenko V.N.;
RT   "Identification and characterization of WhiB-like family proteins of the
RT   Bifidobacterium genus.";
RL   Anaerobe 18:421-429(2012).
CC   -!- FUNCTION: Acts as a transcriptional regulator. Probably redox-
CC       responsive. The apo- but not holo-form probably binds DNA (By
CC       similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster per subunit. Following nitrosylation of
CC       the [4Fe-4S] cluster binds 1 [4Fe-8(NO)] cluster per subunit.
CC       {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: Constitutively expressed during all growth phases. Repressed
CC       by osmotic stress, not responsive to a number of other stimuli.
CC       {ECO:0000269|PubMed:22609519}.
CC   -!- PTM: The Fe-S cluster can be nitrosylated by nitric oxide (NO).
CC       {ECO:0000250}.
CC   -!- PTM: Upon Fe-S cluster removal intramolecular disulfide bonds are
CC       formed. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the WhiB family. {ECO:0000305}.
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DR   EMBL; AE014295; AAN24816.1; -; Genomic_DNA.
DR   RefSeq; NP_696180.1; NC_004307.2.
DR   RefSeq; WP_007051901.1; NC_004307.2.
DR   AlphaFoldDB; Q8G5K1; -.
DR   SMR; Q8G5K1; -.
DR   STRING; 206672.BL1008; -.
DR   EnsemblBacteria; AAN24816; AAN24816; BL1008.
DR   GeneID; 66504853; -.
DR   KEGG; blo:BL1008; -.
DR   PATRIC; fig|206672.9.peg.711; -.
DR   HOGENOM; CLU_106245_4_2_11; -.
DR   OMA; KRICGRC; -.
DR   PhylomeDB; Q8G5K1; -.
DR   Proteomes; UP000000439; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0035731; F:dinitrosyl-iron complex binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01479; WhiB; 1.
DR   InterPro; IPR034768; 4FE4S_WBL.
DR   InterPro; IPR003482; Whib.
DR   PANTHER; PTHR38839; PTHR38839; 1.
DR   Pfam; PF02467; Whib; 1.
DR   PROSITE; PS51674; 4FE4S_WBL; 1.
PE   2: Evidence at transcript level;
KW   4Fe-4S; Cytoplasm; Disulfide bond; DNA-binding; Iron; Iron-sulfur;
KW   Metal-binding; Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..99
FT                   /note="Transcriptional regulator WhiB2"
FT                   /id="PRO_0000420390"
FT   DOMAIN          33..90
FT                   /note="4Fe-4S Wbl-type"
FT   BINDING         34
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         57
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         60
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         66
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   99 AA;  11747 MW;  BD1B512468223321 CRC64;
     MWGVVDDSAE EPWHELWGLF RPDGDVSWQH KALCAQTDPE AFFPEKGGST RDAKRVCAKC
     EVREQCLKWA IDHDERFGIW GGMSERERRR YKREHRERA