WHIB2_MYCS2
ID WHIB2_MYCS2 Reviewed; 129 AA.
AC A0QTG3;
DT 28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=Transcriptional regulator WhiB2;
GN Name=whiB2; Synonyms=whmD; OrderedLocusNames=MSMEG_1831, MSMEI_1789;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [4]
RP INDUCTION.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=20545868; DOI=10.1111/j.1365-2958.2010.07235.x;
RA Rybniker J., Nowag A., van Gumpel E., Nissen N., Robinson N., Plum G.,
RA Hartmann P.;
RT "Insights into the function of the WhiB-like protein of mycobacteriophage
RT TM4--a transcriptional inhibitor of WhiB2.";
RL Mol. Microbiol. 77:642-657(2010).
CC -!- FUNCTION: Acts as a transcriptional regulator. Probably redox-
CC responsive. The apo- but not holo-form probably binds DNA (By
CC similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC Note=Binds 1 [4Fe-4S] cluster per subunit. Following nitrosylation of
CC the [4Fe-4S] cluster binds 1 [4Fe-8(NO)] cluster per subunit.
CC {ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- INDUCTION: Repressed upon infection with mycobacteriophage TM4, and
CC also by expression of the virus paralog whiBTM4 (AC Q9ZX29).
CC {ECO:0000269|PubMed:20545868}.
CC -!- PTM: The Fe-S cluster can be nitrosylated by nitric oxide (NO).
CC {ECO:0000250}.
CC -!- PTM: Upon Fe-S cluster removal intramolecular disulfide bonds are
CC formed. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the WhiB family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; CP000480; ABK74322.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP38261.1; -; Genomic_DNA.
DR RefSeq; WP_003893222.1; NZ_SIJM01000028.1.
DR RefSeq; YP_886201.1; NC_008596.1.
DR AlphaFoldDB; A0QTG3; -.
DR SMR; A0QTG3; -.
DR STRING; 246196.MSMEI_1789; -.
DR EnsemblBacteria; ABK74322; ABK74322; MSMEG_1831.
DR EnsemblBacteria; AFP38261; AFP38261; MSMEI_1789.
DR KEGG; msg:MSMEI_1789; -.
DR KEGG; msm:MSMEG_1831; -.
DR PATRIC; fig|246196.19.peg.1813; -.
DR eggNOG; ENOG5032RUK; Bacteria.
DR OMA; CQGCEVR; -.
DR OrthoDB; 1908599at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0035731; F:dinitrosyl-iron complex binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01479; WhiB; 1.
DR InterPro; IPR034768; 4FE4S_WBL.
DR InterPro; IPR003482; Whib.
DR PANTHER; PTHR38839; PTHR38839; 1.
DR Pfam; PF02467; Whib; 1.
DR PROSITE; PS51674; 4FE4S_WBL; 1.
PE 2: Evidence at transcript level;
KW 4Fe-4S; Cytoplasm; Disulfide bond; DNA-binding; Iron; Iron-sulfur;
KW Metal-binding; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..129
FT /note="Transcriptional regulator WhiB2"
FT /id="PRO_0000420393"
FT DOMAIN 66..123
FT /note="4Fe-4S Wbl-type"
FT REGION 23..45
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 67
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 90
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 93
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 99
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
SQ SEQUENCE 129 AA; 14396 MW; 953A5C10E00CC16C CRC64;
MSYESGDFDR VVRFDNRLLG SVSHAPHIDT GSTPTGAAGR PQLSLVPDSF DVAPEAEEDQ
WQERALCAQT DPEAFFPEKG GSTREAKRIC QGCEVRDACL EYALAHDERF GIWGGLSERE
RRRLKRGII
