WHIB3_MYCS2
ID WHIB3_MYCS2 Reviewed; 96 AA.
AC A0QST8; Q9Z6E9;
DT 28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Redox-responsive transcriptional regulator WhiB3;
GN Name=whiB3; OrderedLocusNames=MSMEG_1597, MSMEI_1558;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], INDUCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=10422690; DOI=10.1016/s0923-2508(99)80055-2;
RA Hutter B., Dick T.;
RT "Molecular genetic characterisation of whiB3, a mycobacterial homologue of
RT a Streptomyces sporulation factor.";
RL Res. Microbiol. 150:295-301(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
CC -!- FUNCTION: A redox-sensitive transcriptional regulator. Maintains
CC intracellular redox homeostasis by regulating catabolic metabolism and
CC polyketide biosynthesis. Regulates expression of the redox buffer
CC ergothioneine (ERG). In concert with myothiol (MSH), another redox
CC buffer, responds to low pH leading to acid resistance. The apo- but not
CC holo-form probably binds DNA (By similarity). {ECO:0000250}.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000250|UniProtKB:P9WF41};
CC Note=Binds 1 [4Fe-4S] cluster per subunit. Following nitrosylation of
CC the [4Fe-4S] cluster binds 1 [4Fe-8(NO)] cluster per subunit.
CC {ECO:0000250|UniProtKB:P9WF41};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- INDUCTION: Constitutively expressed. {ECO:0000269|PubMed:10422690}.
CC -!- PTM: The Fe-S cluster can be nitrosylated by nitric oxide (NO).
CC {ECO:0000250|UniProtKB:P9WF41}.
CC -!- PTM: Upon Fe-S cluster removal intramolecular disulfide bonds are
CC formed. {ECO:0000250|UniProtKB:P9WF41}.
CC -!- DISRUPTION PHENOTYPE: Not essential. {ECO:0000269|PubMed:10422690}.
CC -!- SIMILARITY: Belongs to the WhiB family. {ECO:0000305}.
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DR EMBL; AF073300; AAD19334.1; -; Genomic_DNA.
DR EMBL; CP000480; ABK73466.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP38031.1; -; Genomic_DNA.
DR RefSeq; WP_003893002.1; NZ_SIJM01000023.1.
DR RefSeq; YP_885976.1; NC_008596.1.
DR AlphaFoldDB; A0QST8; -.
DR SMR; A0QST8; -.
DR STRING; 246196.MSMEI_1558; -.
DR EnsemblBacteria; ABK73466; ABK73466; MSMEG_1597.
DR EnsemblBacteria; AFP38031; AFP38031; MSMEI_1558.
DR GeneID; 66733049; -.
DR KEGG; msg:MSMEI_1558; -.
DR KEGG; msm:MSMEG_1597; -.
DR PATRIC; fig|246196.19.peg.1583; -.
DR eggNOG; ENOG5032S23; Bacteria.
DR OMA; WQLHGAC; -.
DR OrthoDB; 1975204at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR GO; GO:0035731; F:dinitrosyl-iron complex binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR HAMAP; MF_01479; WhiB; 1.
DR InterPro; IPR034768; 4FE4S_WBL.
DR InterPro; IPR003482; Whib.
DR PANTHER; PTHR38839; PTHR38839; 1.
DR Pfam; PF02467; Whib; 1.
DR PROSITE; PS51674; 4FE4S_WBL; 1.
PE 2: Evidence at transcript level;
KW 4Fe-4S; Cytoplasm; Disulfide bond; DNA-binding; Iron; Iron-sulfur;
KW Metal-binding; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..96
FT /note="Redox-responsive transcriptional regulator WhiB3"
FT /id="PRO_0000420394"
FT DOMAIN 22..86
FT /note="4Fe-4S Wbl-type"
FT BINDING 23
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250|UniProtKB:P9WF41"
FT BINDING 53
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250|UniProtKB:P9WF41"
FT BINDING 56
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250|UniProtKB:P9WF41"
FT BINDING 62
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250|UniProtKB:P9WF41"
SQ SEQUENCE 96 AA; 10881 MW; C5348C479195BE33 CRC64;
MPQPQQLPGP NADIWDWQMR GLCRGVDSSM FFHPDGERGR ARAQREMRAK EMCRSCPVIA
QCRSHALAVG EPYGIWGGLS ESERELLLKR GIRRSA
