ID   WHIB4_MYCTO             Reviewed;         118 AA.
AC   P9WF38; F2GFC4; L0TGF3; O69649; Q7D530;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 39.
DE   RecName: Full=Transcriptional regulator WhiB4;
GN   Name=whiB4; OrderedLocusNames=MT3783;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
RN   [2]
RP   INDUCTION.
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=16870781; DOI=10.1128/aac.00295-06;
RA   Geiman D.E., Raghunand T.R., Agarwal N., Bishai W.R.;
RT   "Differential gene expression in response to exposure to antimycobacterial
RT   agents and other stress conditions among seven Mycobacterium tuberculosis
RT   whiB-like genes.";
RL   Antimicrob. Agents Chemother. 50:2836-2841(2006).
RN   [3]
RP   INDUCTION.
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=22829866; DOI=10.1371/journal.pone.0037516;
RA   Larsson C., Luna B., Ammerman N.C., Maiga M., Agarwal N., Bishai W.R.;
RT   "Gene expression of Mycobacterium tuberculosis putative transcription
RT   factors whiB1-7 in redox environments.";
RL   PLoS ONE 7:E37516-E37516(2012).
CC   -!- FUNCTION: Acts as a transcriptional regulator. Probably redox-
CC       responsive. The apo- but not holo-form probably binds DNA (By
CC       similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster per subunit. Following nitrosylation of
CC       the [4Fe-4S] cluster binds 1 [4Fe-8(NO)] cluster per subunit.
CC       {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: Essentially constitutive over all growth phases. 2-fold
CC       repressed by ethanol, 4-fold by SDS. Not induced by hypoxia, slightly
CC       induced by NO, cAMP, 8-fold induced in mouse lung infection.
CC       {ECO:0000269|PubMed:16870781, ECO:0000269|PubMed:22829866}.
CC   -!- PTM: The Fe-S cluster can be nitrosylated by nitric oxide (NO).
CC       {ECO:0000250}.
CC   -!- PTM: Upon Fe-S cluster removal intramolecular disulfide bonds are
CC       formed. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the WhiB family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK48150.1; -; Genomic_DNA.
DR   PIR; B70791; B70791.
DR   RefSeq; WP_003419743.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WF38; -.
DR   SMR; P9WF38; -.
DR   EnsemblBacteria; AAK48150; AAK48150; MT3783.
DR   GeneID; 45427677; -.
DR   KEGG; mtc:MT3783; -.
DR   PATRIC; fig|83331.31.peg.4074; -.
DR   HOGENOM; CLU_106245_2_3_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0035731; F:dinitrosyl-iron complex binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01479; WhiB; 1.
DR   InterPro; IPR034768; 4FE4S_WBL.
DR   InterPro; IPR003482; Whib.
DR   PANTHER; PTHR38839; PTHR38839; 1.
DR   Pfam; PF02467; Whib; 1.
DR   PROSITE; PS51674; 4FE4S_WBL; 1.
PE   2: Evidence at transcript level;
KW   4Fe-4S; Cytoplasm; Disulfide bond; DNA-binding; Iron; Iron-sulfur;
KW   Metal-binding; Transcription; Transcription regulation.
FT   CHAIN           1..118
FT                   /note="Transcriptional regulator WhiB4"
FT                   /id="PRO_0000428606"
FT   DOMAIN          36..92
FT                   /note="4Fe-4S Wbl-type"
FT   BINDING         37
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         59
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         62
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         68
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   DISULFID        37..68
FT                   /note="Upon loss of 4Fe-S clusters"
FT                   /evidence="ECO:0000250"
FT   DISULFID        59..62
FT                   /note="Upon loss of 4Fe-S clusters"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   118 AA;  13211 MW;  6E6451A2714B859B CRC64;
     MSGTRPAARR TNLTAAQNVV RSVDAEERIA WVSKALCRTT DPDELFVRGA AQRKAAVICR
     HCPVMQECAA DALDNKVEFG VWGGMTERQR RALLKQHPEV VSWSDYLEKR KRRTGTAG