ID   WHIB6_MYCTO             Reviewed;         116 AA.
AC   P9WF36; F2GDM3; L0TGU1; P96215; Q7D4Q1;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 37.
DE   RecName: Full=Probable transcriptional regulator WhiB6;
GN   Name=whiB6; OrderedLocusNames=MT3976;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
RN   [2]
RP   INDUCTION.
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=16870781; DOI=10.1128/aac.00295-06;
RA   Geiman D.E., Raghunand T.R., Agarwal N., Bishai W.R.;
RT   "Differential gene expression in response to exposure to antimycobacterial
RT   agents and other stress conditions among seven Mycobacterium tuberculosis
RT   whiB-like genes.";
RL   Antimicrob. Agents Chemother. 50:2836-2841(2006).
RN   [3]
RP   INDUCTION.
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=22829866; DOI=10.1371/journal.pone.0037516;
RA   Larsson C., Luna B., Ammerman N.C., Maiga M., Agarwal N., Bishai W.R.;
RT   "Gene expression of Mycobacterium tuberculosis putative transcription
RT   factors whiB1-7 in redox environments.";
RL   PLoS ONE 7:E37516-E37516(2012).
CC   -!- FUNCTION: Acts as a transcriptional regulator. Probably redox-
CC       responsive. The apo- but not holo-form probably binds DNA (By
CC       similarity). {ECO:0000250}.
CC   -!- COFACTOR:
CC       Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000250};
CC       Note=Binds 1 [4Fe-4S] cluster per subunit. Following nitrosylation of
CC       the [4Fe-4S] cluster binds 1 [4Fe-8(NO)] cluster per subunit.
CC       {ECO:0000250};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: Weakly expressed in exponential phase, more induced upon
CC       entry into stationary phase. 3-fold induced by streptomycin and pH 4.5,
CC       4-fold by ethanol. Slightly induced during entry into hypoxia, by cAMP,
CC       in macrophage infection and 5-fold induced by NO. Repressed by WhiB4.
CC       {ECO:0000269|PubMed:16870781, ECO:0000269|PubMed:22829866}.
CC   -!- PTM: The Fe-S cluster can be nitrosylated by nitric oxide (NO).
CC       {ECO:0000250}.
CC   -!- PTM: Upon Fe-S cluster removal intramolecular disulfide bonds are
CC       formed. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the WhiB family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK48345.1; -; Genomic_DNA.
DR   PIR; C70656; C70656.
DR   RefSeq; WP_003899734.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WF36; -.
DR   SMR; P9WF36; -.
DR   EnsemblBacteria; AAK48345; AAK48345; MT3976.
DR   GeneID; 45427866; -.
DR   KEGG; mtc:MT3976; -.
DR   HOGENOM; CLU_167541_0_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0035731; F:dinitrosyl-iron complex binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01479; WhiB; 1.
DR   InterPro; IPR034768; 4FE4S_WBL.
DR   InterPro; IPR003482; Whib.
DR   Pfam; PF02467; Whib; 1.
DR   PROSITE; PS51674; 4FE4S_WBL; 1.
PE   2: Evidence at transcript level;
KW   4Fe-4S; Cytoplasm; Disulfide bond; DNA-binding; Iron; Iron-sulfur;
KW   Metal-binding; Transcription; Transcription regulation.
FT   CHAIN           1..116
FT                   /note="Probable transcriptional regulator WhiB6"
FT                   /id="PRO_0000428607"
FT   DOMAIN          33..86
FT                   /note="4Fe-4S Wbl-type"
FT   BINDING         12
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         53
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         56
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
FT   BINDING         62
FT                   /ligand="[4Fe-4S] cluster"
FT                   /ligand_id="ChEBI:CHEBI:49883"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   116 AA;  12792 MW;  7FDD5ABBD7F22A94 CRC64;
     MRYAFAAEAT TCNAFWRNVD MTVTALYEVP LGVCTQDPDR WTTTPDDEAK TLCRACPRRW
     LCARDAVESA GAEGLWAGVV IPESGRARAF ALGQLRSLAE RNGYPVRDHR VSAQSA