WHIB_BPMT4
ID WHIB_BPMT4 Reviewed; 76 AA.
AC Q9ZX29;
DT 28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 61.
DE RecName: Full=Probable transcriptional regulator WhiBTM4;
GN Name=whiBTM4; Synonyms=49; ORFNames=TM4_49;
OS Mycobacterium phage TM4 (Mycobacteriophage TM4).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Siphoviridae; Timquatrovirus.
OX NCBI_TaxID=88870;
OH NCBI_TaxID=1763; Mycobacterium.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10645443; DOI=10.1054/tuld.1998.0007;
RA Ford M.E., Stenstrom C., Hendrix R.W., Hatfull G.F.;
RT "Mycobacteriophage TM4: genome structure and gene expression.";
RL Tuber. Lung Dis. 79:63-73(1998).
RN [2]
RP FUNCTION, COFACTOR, SUBUNIT, DNA-BINDING, INDUCTION, DISRUPTION PHENOTYPE,
RP AND MUTAGENESIS OF CYS-36; CYS-39; CYS-45; LEU-55; VAL-56; VAL-58; TRP-59
RP AND ARG-67.
RX PubMed=20545868; DOI=10.1111/j.1365-2958.2010.07235.x;
RA Rybniker J., Nowag A., van Gumpel E., Nissen N., Robinson N., Plum G.,
RA Hartmann P.;
RT "Insights into the function of the WhiB-like protein of mycobacteriophage
RT TM4--a transcriptional inhibitor of WhiB2.";
RL Mol. Microbiol. 77:642-657(2010).
CC -!- FUNCTION: A dominant-negative regulator of endogenous whiB2 expression.
CC Low level expression in liquid culture in M.smegmatis leads to
CC elongated and highly branched cells, with multiple nucleoids, similar
CC to the effects seen in deletion experiments for whiB2 in M.smegmatis,
CC as well as loss of acid-fastness. High level expression is lethal.
CC Represses expression of endogenous whiB2. The apo-form binds to the
CC whiB2 promoter found in M.tuberculosis. M.smegmatis cells expressing
CC this protein will not grow on plates. Low level expression in
CC M.smegmatis leads to superinfection exclusion, possibly via
CC modification to the cell wall. {ECO:0000269|PubMed:20545868}.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000250, ECO:0000305};
CC Note=Binds 1 [4Fe-4S] cluster per subunit. Contains 1 [2Fe-2S] cluster
CC after reconstitution of overexpressed protein from E.coli. Following
CC nitrosylation of the [4Fe-4S] cluster binds 1 [4Fe-8(NO)] cluster per
CC subunit. {ECO:0000250, ECO:0000305};
CC -!- SUBUNIT: Forms homodimers in the absence of a reducing agent.
CC {ECO:0000269|PubMed:20545868}.
CC -!- SUBCELLULAR LOCATION: Host cytoplasm {ECO:0000305}.
CC -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC {ECO:0000269|PubMed:20545868}.
CC -!- PTM: The iron-sulfur cluster is sensitive to air and oxidizing agents;
CC degradation is complete in 3 hours upon exposure to air.
CC -!- PTM: The Fe-S cluster can be nitrosylated by nitric oxide (NO).
CC {ECO:0000250}.
CC -!- PTM: Upon Fe-S cluster removal intramolecular disulfide bonds are
CC formed. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Dispensible for viral growth in M.smegmatis
CC strain ATCC 700084 / mc(2)155. {ECO:0000269|PubMed:20545868}.
CC -!- SIMILARITY: Belongs to the WhiB family. {ECO:0000305}.
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DR EMBL; AF068845; AAD17616.1; -; Genomic_DNA.
DR RefSeq; NP_569784.1; NC_003387.1.
DR SMR; Q9ZX29; -.
DR GeneID; 932296; -.
DR KEGG; vg:932296; -.
DR Proteomes; UP000002133; Genome.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:CACAO.
DR HAMAP; MF_01479; WhiB; 1.
DR InterPro; IPR034768; 4FE4S_WBL.
DR InterPro; IPR003482; Whib.
DR PANTHER; PTHR38839; PTHR38839; 1.
DR Pfam; PF02467; Whib; 1.
DR PROSITE; PS51674; 4FE4S_WBL; 1.
PE 1: Evidence at protein level;
KW 4Fe-4S; Disulfide bond; DNA-binding; Host cytoplasm; Iron; Iron-sulfur;
KW Metal-binding; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..76
FT /note="Probable transcriptional regulator WhiBTM4"
FT /id="PRO_0000420399"
FT DOMAIN 12..69
FT /note="4Fe-4S Wbl-type"
FT BINDING 13
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000305"
FT BINDING 36
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000305"
FT BINDING 39
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000250"
FT BINDING 45
FT /ligand="[4Fe-4S] cluster"
FT /ligand_id="ChEBI:CHEBI:49883"
FT /evidence="ECO:0000305"
FT MUTAGEN 36
FT /note="C->S: Unstable iron-sulfur center, small orange
FT colonies in M.smegmatis. Binds DNA."
FT /evidence="ECO:0000269|PubMed:20545868"
FT MUTAGEN 39
FT /note="C->F,R: No iron-sulfur cluster, normal white
FT colonies in M.smegmatis."
FT /evidence="ECO:0000269|PubMed:20545868"
FT MUTAGEN 45
FT /note="C->S: Unstable iron-sulfur center, small orange
FT colonies in M.smegmatis. Binds DNA."
FT /evidence="ECO:0000269|PubMed:20545868"
FT MUTAGEN 55
FT /note="L->Q: Small orange colonies in M.smegmatis.
FT Decreased DNA-binding."
FT /evidence="ECO:0000269|PubMed:20545868"
FT MUTAGEN 56
FT /note="V->D: Small orange colonies in M.smegmatis. No DNA-
FT binding."
FT /evidence="ECO:0000269|PubMed:20545868"
FT MUTAGEN 58
FT /note="V->E: Small orange colonies in M.smegmatis, poor
FT DNA-binding. Decreased DNA-binding."
FT /evidence="ECO:0000269|PubMed:20545868"
FT MUTAGEN 59
FT /note="W->C: Small orange colonies in M.smegmatis.
FT Decreased DNA-binding."
FT /evidence="ECO:0000269|PubMed:20545868"
FT MUTAGEN 67
FT /note="R->C: Small orange colonies in M.smegmatis.
FT Decreased DNA-binding."
FT /evidence="ECO:0000269|PubMed:20545868"
SQ SEQUENCE 76 AA; 8491 MW; 3005C683CD4F9352 CRC64;
MHMHMGGDPS AICAQTDPEL WFPDKGQSTR DAKRMCMRCP LLDECRALAL RDPHLVGVWG
GLSAQERRRI RKGASA
