WHW2_WHEAT
ID WHW2_WHEAT Reviewed; 148 AA.
AC O64393;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=Wheatwin-2;
DE AltName: Full=Pathogenesis-related protein 4b;
DE Flags: Precursor;
GN Name=PR4B;
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. San Pastore; TISSUE=Endosperm;
RX PubMed=10727084; DOI=10.3109/10425179909033956;
RA Caruso C., Bertini L., Tucci M., Caporale C., Leonardi L., Saccardo F.,
RA Bressan R.A., Veronese P., Buonocore V.;
RT "Isolation and characterisation of wheat cDNA clones encoding PR4
RT proteins.";
RL DNA Seq. 10:301-307(1999).
RN [2]
RP PROTEIN SEQUENCE OF 24-148, PYROGLUTAMATE FORMATION AT GLN-24, AND
RP ANTIFUNGAL ACTIVITY.
RC STRAIN=cv. San Pastore; TISSUE=Kernel;
RX PubMed=8838588; DOI=10.1007/bf01886809;
RA Caruso C., Caporale C., Chilosi G., Vacca F., Bertini L., Magro P.,
RA Poerio E., Buonocore V.;
RT "Structural and antifungal properties of a pathogenesis-related protein
RT from wheat kernel.";
RL J. Protein Chem. 15:35-44(1996).
CC -!- FUNCTION: Shows antifungal activity towards B.cinerea and towards the
CC wheat-specific pathogenic fungi F.culmorum and F.graminearum (groups 1
CC and 2).
CC -!- SUBUNIT: Monomer.
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DR EMBL; AJ006099; CAA06857.1; -; Genomic_DNA.
DR PIR; A53882; A53882.
DR PIR; T06486; T06486.
DR AlphaFoldDB; O64393; -.
DR SMR; O64393; -.
DR STRING; 4565.Traes_3B_F0BA991CF.1; -.
DR PRIDE; O64393; -.
DR EnsemblPlants; TraesCAD_scaffold_092477_01G000100.1; TraesCAD_scaffold_092477_01G000100.1; TraesCAD_scaffold_092477_01G000100.
DR EnsemblPlants; TraesCLE_scaffold_038497_01G000100.1; TraesCLE_scaffold_038497_01G000100.1; TraesCLE_scaffold_038497_01G000100.
DR EnsemblPlants; TraesCS3D02G524700.1; TraesCS3D02G524700.1.cds1; TraesCS3D02G524700.
DR EnsemblPlants; TraesPAR_scaffold_130615_01G000100.1; TraesPAR_scaffold_130615_01G000100.1; TraesPAR_scaffold_130615_01G000100.
DR EnsemblPlants; TraesROB_scaffold_012445_01G001200.1; TraesROB_scaffold_012445_01G001200.1; TraesROB_scaffold_012445_01G001200.
DR EnsemblPlants; TraesWEE_scaffold_112963_01G000100.1; TraesWEE_scaffold_112963_01G000100.1; TraesWEE_scaffold_112963_01G000100.
DR Gramene; TraesCAD_scaffold_092477_01G000100.1; TraesCAD_scaffold_092477_01G000100.1; TraesCAD_scaffold_092477_01G000100.
DR Gramene; TraesCLE_scaffold_038497_01G000100.1; TraesCLE_scaffold_038497_01G000100.1; TraesCLE_scaffold_038497_01G000100.
DR Gramene; TraesCS3D02G524700.1; TraesCS3D02G524700.1.cds1; TraesCS3D02G524700.
DR Gramene; TraesPAR_scaffold_130615_01G000100.1; TraesPAR_scaffold_130615_01G000100.1; TraesPAR_scaffold_130615_01G000100.
DR Gramene; TraesROB_scaffold_012445_01G001200.1; TraesROB_scaffold_012445_01G001200.1; TraesROB_scaffold_012445_01G001200.
DR Gramene; TraesWEE_scaffold_112963_01G000100.1; TraesWEE_scaffold_112963_01G000100.1; TraesWEE_scaffold_112963_01G000100.
DR eggNOG; KOG4742; Eukaryota.
DR OMA; HAQGHLM; -.
DR Proteomes; UP000019116; Unplaced.
DR GO; GO:0004540; F:ribonuclease activity; IEA:InterPro.
DR GO; GO:0042742; P:defense response to bacterium; IEA:InterPro.
DR GO; GO:0050832; P:defense response to fungus; IEA:UniProtKB-KW.
DR GO; GO:0031640; P:killing of cells of another organism; IEA:UniProtKB-KW.
DR GO; GO:0009626; P:plant-type hypersensitive response; IEA:UniProtKB-KW.
DR Gene3D; 2.40.40.10; -; 1.
DR InterPro; IPR018226; Barwin_CS.
DR InterPro; IPR001153; Barwin_dom.
DR InterPro; IPR044301; PR4.
DR InterPro; IPR036908; RlpA-like_sf.
DR PANTHER; PTHR46351; PTHR46351; 1.
DR Pfam; PF00967; Barwin; 1.
DR PRINTS; PR00602; BARWIN.
DR SUPFAM; SSF50685; SSF50685; 1.
DR PROSITE; PS00771; BARWIN_1; 1.
DR PROSITE; PS00772; BARWIN_2; 1.
DR PROSITE; PS51174; BARWIN_3; 1.
PE 1: Evidence at protein level;
KW Antimicrobial; Direct protein sequencing; Disulfide bond; Fungicide;
KW Hypersensitive response; Pathogenesis-related protein; Plant defense;
KW Pyrrolidone carboxylic acid; Reference proteome; Signal.
FT SIGNAL 1..23
FT /evidence="ECO:0000269|PubMed:8838588"
FT CHAIN 24..148
FT /note="Wheatwin-2"
FT /id="PRO_0000002794"
FT DOMAIN 24..148
FT /note="Barwin"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00527"
FT MOD_RES 24
FT /note="Pyrrolidone carboxylic acid"
FT /evidence="ECO:0000269|PubMed:8838588"
FT DISULFID 54..86
FT /evidence="ECO:0000250"
FT DISULFID 75..109
FT /evidence="ECO:0000250"
FT DISULFID 89..146
FT /evidence="ECO:0000250"
FT CONFLICT 111
FT /note="N -> D (in Ref. 2; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 148 AA; 15867 MW; 3D4EA32E085BCBC1 CRC64;
MTMAARLMLV AALLCAAAAA ATAQQATNVR ATYHYYRPAQ NNWDLGAPAV SAYCATWDAS
KPLSWRSKYG WTAFCGPAGA HGQAACGKCL RVTNPATGAQ ITARIVDQCA NGGLDLDWDT
VFTKIDTNGI GYQQGHLNVN YQFVDCRD
