WHY1_MAIZE
ID WHY1_MAIZE Reviewed; 266 AA.
AC B2LXS7;
DT 28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 1.
DT 03-AUG-2022, entry version 74.
DE RecName: Full=Single-stranded DNA-binding protein WHY1, chloroplastic;
DE AltName: Full=Protein WHIRLY 1;
DE Short=ZmWHY1;
DE Flags: Precursor;
GN Name=WHY1;
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=cv. B73;
RX PubMed=18676978; DOI=10.1093/nar/gkn492;
RA Prikryl J., Watkins K.P., Friso G., van Wijk K.J., Barkan A.;
RT "A member of the Whirly family is a multifunctional RNA- and DNA-binding
RT protein that is essential for chloroplast biogenesis.";
RL Nucleic Acids Res. 36:5152-5165(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=18937034; DOI=10.1007/s11103-008-9415-4;
RA Alexandrov N.N., Brover V.V., Freidin S., Troukhan M.E., Tatarinova T.V.,
RA Zhang H., Swaller T.J., Lu Y.-P., Bouck J., Flavell R.B., Feldmann K.A.;
RT "Insights into corn genes derived from large-scale cDNA sequencing.";
RL Plant Mol. Biol. 69:179-194(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. B73;
RX PubMed=19936069; DOI=10.1371/journal.pgen.1000740;
RA Soderlund C., Descour A., Kudrna D., Bomhoff M., Boyd L., Currie J.,
RA Angelova A., Collura K., Wissotski M., Ashley E., Morrow D., Fernandes J.,
RA Walbot V., Yu Y.;
RT "Sequencing, mapping, and analysis of 27,455 maize full-length cDNAs.";
RL PLoS Genet. 5:E1000740-E1000740(2009).
RN [4]
RP GENE FAMILY.
RX PubMed=15708347; DOI=10.1016/j.tplants.2004.12.008;
RA Desveaux D., Marechal A., Brisson N.;
RT "Whirly transcription factors: defense gene regulation and beyond.";
RL Trends Plant Sci. 10:95-102(2005).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19666500; DOI=10.1073/pnas.0901710106;
RA Marechal A., Parent J.S., Veronneau-Lafortune F., Joyeux A., Lang B.F.,
RA Brisson N.;
RT "Whirly proteins maintain plastid genome stability in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 106:14693-14698(2009).
CC -!- FUNCTION: Single-stranded DNA and RNA binding protein that maintains
CC plastid genome stability by preventing break-induced and short
CC homology-dependent illegitimate recombinations. Functions in RNA
CC metabolism and is involved in the maturation of the atpF and 23S
CC ribosomal RNAs. {ECO:0000269|PubMed:18676978,
CC ECO:0000269|PubMed:19666500}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma
CC {ECO:0000269|PubMed:18676978}. Nucleus {ECO:0000250|UniProtKB:Q9M9S3}.
CC Note=Can localize to both chloroplast and nucleus.
CC {ECO:0000250|UniProtKB:Q9M9S3}.
CC -!- DISRUPTION PHENOTYPE: Albino seedlings lacking plastid ribosomes.
CC {ECO:0000269|PubMed:18676978, ECO:0000269|PubMed:19666500}.
CC -!- SIMILARITY: Belongs to the Whirly family. {ECO:0000305}.
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DR EMBL; EU595664; ACC60344.1; -; mRNA.
DR EMBL; EU955861; ACG27979.1; -; mRNA.
DR EMBL; BT043360; ACF88365.1; -; mRNA.
DR RefSeq; NP_001123589.1; NM_001130117.1.
DR AlphaFoldDB; B2LXS7; -.
DR SMR; B2LXS7; -.
DR STRING; 4577.GRMZM2G155662_P01; -.
DR PaxDb; B2LXS7; -.
DR PRIDE; B2LXS7; -.
DR EnsemblPlants; Zm00001eb270470_T002; Zm00001eb270470_P002; Zm00001eb270470.
DR GeneID; 100170235; -.
DR Gramene; Zm00001eb270470_T002; Zm00001eb270470_P002; Zm00001eb270470.
DR KEGG; zma:100170235; -.
DR MaizeGDB; 1233241; -.
DR eggNOG; ENOG502QRRY; Eukaryota.
DR HOGENOM; CLU_062935_1_0_1; -.
DR OrthoDB; 1637760at2759; -.
DR Proteomes; UP000007305; Chromosome 6.
DR ExpressionAtlas; B2LXS7; baseline and differential.
DR GO; GO:0009570; C:chloroplast stroma; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB.
DR GO; GO:0003723; F:RNA binding; IDA:UniProtKB.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR GO; GO:0006952; P:defense response; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR GO; GO:0045910; P:negative regulation of DNA recombination; IMP:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 2.30.31.10; -; 1.
DR InterPro; IPR009044; ssDNA-bd_transcriptional_reg.
DR InterPro; IPR013742; Whirly.
DR PANTHER; PTHR31745; PTHR31745; 1.
DR Pfam; PF08536; Whirly; 1.
DR SUPFAM; SSF54447; SSF54447; 1.
PE 2: Evidence at transcript level;
KW Chloroplast; DNA damage; DNA repair; DNA-binding; Nucleus; Plastid;
KW Reference proteome; RNA-binding; Transit peptide.
FT TRANSIT 1..37
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 38..266
FT /note="Single-stranded DNA-binding protein WHY1,
FT chloroplastic"
FT /id="PRO_0000420452"
FT REGION 53..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 94..99
FT /note="Required for ssDNA binding"
FT /evidence="ECO:0000250"
FT MOTIF 172..185
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
SQ SEQUENCE 266 AA; 29439 MW; 225FE5FF6168EADC CRC64;
MPPPAPLFLS LASTPPPALM PVHHPRAPQS LTLVPPVASS RKAAAVPACP VASPRHSDYF
DPRAPPPPRG DGGYGRPPNG AQDGRVFTSY SIYKGKAALS FDPRPPLFVP LDSGAYKVAK
EGFVLLQFAP AVATRQYDWT RKQVFSLSVW EIGTLLTLGP TDSCEFFHDP FKGRSEEGKV
RKVLKIEPTP DGNGRFFNLS VQNRLINVDE SIYIPITKGE FAVIVSTFNY IIPHLMGWST
FVSSIKPEES RPYSRPQSTS EYEWRR
