WHY1_SOLTU
ID WHY1_SOLTU Reviewed; 274 AA.
AC Q9LL85;
DT 28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 25-MAY-2022, entry version 92.
DE RecName: Full=Single-stranded DNA-binding protein WHY1, chloroplastic;
DE AltName: Full=DNA-binding protein p24;
DE AltName: Full=PR-10a binding factor 2;
DE Short=PBF-2;
DE AltName: Full=Protein WHIRLY 1;
DE Short=StWhy1;
DE Flags: Precursor;
GN Name=WHY1;
OS Solanum tuberosum (Potato).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX NCBI_TaxID=4113;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 111-123 AND 192-198,
RP FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Kennebec;
RX PubMed=10948264; DOI=10.2307/3871144;
RA Desveaux D., Despres C., Joyeux A., Subramaniam R., Brisson N.;
RT "PBF-2 is a novel single-stranded DNA binding factor implicated in PR-10a
RT gene activation in potato.";
RL Plant Cell 12:1477-1489(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. DM1-3 516 R44;
RX PubMed=21743474; DOI=10.1038/nature10158;
RG The Potato Genome Sequencing Consortium;
RT "Genome sequence and analysis of the tuber crop potato.";
RL Nature 475:189-195(2011).
RN [3]
RP FUNCTION.
RX PubMed=14960277; DOI=10.1016/s1534-5807(04)00028-0;
RA Desveaux D., Subramaniam R., Despres C., Mess J.N., Levesque C.,
RA Fobert P.R., Dangl J.L., Brisson N.;
RT "A 'Whirly' transcription factor is required for salicylic acid-dependent
RT disease resistance in Arabidopsis.";
RL Dev. Cell 6:229-240(2004).
RN [4]
RP GENE FAMILY.
RX PubMed=15708347; DOI=10.1016/j.tplants.2004.12.008;
RA Desveaux D., Marechal A., Brisson N.;
RT "Whirly transcription factors: defense gene regulation and beyond.";
RL Trends Plant Sci. 10:95-102(2005).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.30 ANGSTROMS) OF 68-273 IN COMPLEX WITH
RP SINGLE-STRANDED DNA, FUNCTION, SUBUNIT, AND MUTAGENESIS OF
RP 100-LYS--LEU-105.
RX PubMed=12080340; DOI=10.1038/nsb814;
RA Desveaux D., Allard J., Brisson N., Sygusch J.;
RT "A new family of plant transcription factors displays a novel ssDNA-binding
RT surface.";
RL Nat. Struct. Biol. 9:512-517(2002).
CC -!- FUNCTION: Single-stranded DNA-binding protein that acts as a
CC transcriptional activator of the pathogenesis-related gene PR-10a. Upon
CC elicitation, binds a 30bp promoter sequence known as elicitor element
CC response (ERE) and is required for PR-10a expression.
CC {ECO:0000269|PubMed:10948264, ECO:0000269|PubMed:12080340,
CC ECO:0000269|PubMed:14960277}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000269|PubMed:12080340}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10948264}. Plastid,
CC chloroplast {ECO:0000250|UniProtKB:Q9M9S3}. Note=Can localize to both
CC chloroplast and nucleus. {ECO:0000250|UniProtKB:Q9M9S3}.
CC -!- SIMILARITY: Belongs to the Whirly family. {ECO:0000305}.
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DR EMBL; AF233342; AAF91282.1; -; mRNA.
DR RefSeq; NP_001275155.1; NM_001288226.1.
DR PDB; 1L3A; X-ray; 2.30 A; A/B/C/D=68-273.
DR PDBsum; 1L3A; -.
DR AlphaFoldDB; Q9LL85; -.
DR SMR; Q9LL85; -.
DR STRING; 4113.PGSC0003DMT400045439; -.
DR EnsemblPlants; PGSC0003DMT400045439; PGSC0003DMT400045439; PGSC0003DMG400017627.
DR EnsemblPlants; RHC05H1G0698.2.1; RHC05H1G0698.2.1; RHC05H1G0698.2.
DR GeneID; 102577544; -.
DR Gramene; PGSC0003DMT400045439; PGSC0003DMT400045439; PGSC0003DMG400017627.
DR Gramene; RHC05H1G0698.2.1; RHC05H1G0698.2.1; RHC05H1G0698.2.
DR KEGG; sot:102577544; -.
DR eggNOG; ENOG502QRRY; Eukaryota.
DR HOGENOM; CLU_062935_1_0_1; -.
DR InParanoid; Q9LL85; -.
DR OMA; KESCEFF; -.
DR OrthoDB; 1637760at2759; -.
DR EvolutionaryTrace; Q9LL85; -.
DR Proteomes; UP000011115; Unassembled WGS sequence.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR GO; GO:0050832; P:defense response to fungus; TAS:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR Gene3D; 2.30.31.10; -; 1.
DR InterPro; IPR009044; ssDNA-bd_transcriptional_reg.
DR InterPro; IPR013742; Whirly.
DR PANTHER; PTHR31745; PTHR31745; 1.
DR Pfam; PF08536; Whirly; 1.
DR SUPFAM; SSF54447; SSF54447; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Activator; Chloroplast; Direct protein sequencing;
KW DNA-binding; Nucleus; Plant defense; Plastid; Reference proteome;
KW Transcription; Transcription regulation; Transit peptide.
FT TRANSIT 1..54
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 55..274
FT /note="Single-stranded DNA-binding protein WHY1,
FT chloroplastic"
FT /id="PRO_0000420450"
FT REGION 100..105
FT /note="Required for ssDNA binding"
FT REGION 253..274
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 178..191
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 253..268
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 100..105
FT /note="KGKAAL->QGQGGV: Loss of ssDNA binding."
FT /evidence="ECO:0000269|PubMed:12080340"
FT STRAND 96..99
FT /evidence="ECO:0007829|PDB:1L3A"
FT STRAND 101..110
FT /evidence="ECO:0007829|PDB:1L3A"
FT STRAND 113..116
FT /evidence="ECO:0007829|PDB:1L3A"
FT STRAND 121..126
FT /evidence="ECO:0007829|PDB:1L3A"
FT STRAND 129..137
FT /evidence="ECO:0007829|PDB:1L3A"
FT HELIX 145..147
FT /evidence="ECO:0007829|PDB:1L3A"
FT STRAND 149..153
FT /evidence="ECO:0007829|PDB:1L3A"
FT HELIX 155..162
FT /evidence="ECO:0007829|PDB:1L3A"
FT STRAND 170..174
FT /evidence="ECO:0007829|PDB:1L3A"
FT STRAND 179..181
FT /evidence="ECO:0007829|PDB:1L3A"
FT STRAND 187..194
FT /evidence="ECO:0007829|PDB:1L3A"
FT STRAND 198..209
FT /evidence="ECO:0007829|PDB:1L3A"
FT HELIX 210..212
FT /evidence="ECO:0007829|PDB:1L3A"
FT STRAND 214..223
FT /evidence="ECO:0007829|PDB:1L3A"
FT HELIX 224..241
FT /evidence="ECO:0007829|PDB:1L3A"
FT HELIX 244..248
FT /evidence="ECO:0007829|PDB:1L3A"
SQ SEQUENCE 274 AA; 30327 MW; A4DDF3CD4045A5A2 CRC64;
MSNFSLSPSP TSGFSLNLQN PTKTSYLSFS SSINTIFAPL SSNTTKSFSG LTHKAALPRN
LSLTCRHSDY FEPQQQQQQQ QQQPQGASTP KVFVGYSIYK GKAALTVEPR SPEFSPLDSG
AFKLSREGMV MLQFAPAAGV RQYDWSRKQV FSLSVTEIGS IISLGAKDSC EFFHDPNKGR
SDEGRVRKVL KVEPLPDGSG HFFNLSVQNK LINLDENIYI PVTKAEFAVL VSAFNFVMPY
LLGWHTAVNS FKPEDASRSN NANPRSGAEL EWNR
