WIH2_ARATH
ID WIH2_ARATH Reviewed; 98 AA.
AC Q8S8M0;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=Cysteine-rich and transmembrane domain-containing protein WIH2 {ECO:0000305};
DE AltName: Full=Cysteine-rich and transmembrane domain-containing protein A {ECO:0000305};
DE AltName: Full=Protein WINDHOSE 2 {ECO:0000303|PubMed:21658947};
GN Name=WIH2 {ECO:0000303|PubMed:21658947};
GN OrderedLocusNames=At2g41420 {ECO:0000312|Araport:AT2G41420};
GN ORFNames=T26J13.1 {ECO:0000312|EMBL:AAM15069.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=15060130; DOI=10.1074/mcp.m400001-mcp200;
RA Marmagne A., Rouet M.-A., Ferro M., Rolland N., Alcon C., Joyard J.,
RA Garin J., Barbier-Brygoo H., Ephritikhine G.;
RT "Identification of new intrinsic proteins in Arabidopsis plasma membrane
RT proteome.";
RL Mol. Cell. Proteomics 3:675-691(2004).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=21658947; DOI=10.1016/j.cub.2011.05.015;
RA Lieber D., Lora J., Schrempp S., Lenhard M., Laux T.;
RT "Arabidopsis WIH1 and WIH2 genes act in the transition from somatic to
RT reproductive cell fate.";
RL Curr. Biol. 21:1009-1017(2011).
CC -!- FUNCTION: Required for the promotion of megasporogenesis, or promotion
CC of germ cell formation from somatic precursor cells. Acts redundantly
CC with WIH1. Functions in a genetic pathway downstream of SPL/NZZ and WUS
CC and together with TRN2 in promoting megasporogenesis.
CC {ECO:0000269|PubMed:21658947}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000269|PubMed:15060130}; Single-
CC pass membrane protein {ECO:0000255}.
CC -!- TISSUE SPECIFICITY: Expressed in floral organ primordia.
CC {ECO:0000269|PubMed:21658947}.
CC -!- DEVELOPMENTAL STAGE: During ovule development, expressed in the
CC nucellus from early stage until embryo sac maturity. In mature ovules,
CC expressed in the nucellus and the outer integument.
CC {ECO:0000269|PubMed:21658947}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype under normal growth
CC conditions, but flowers of the double mutants wih1-1 and wih2-1 have
CC defect in megasporgogenesis. Doubl mutant plants display retarded
CC growth, and twisted leaves, siliques and roots.
CC {ECO:0000269|PubMed:21658947}.
CC -!- SIMILARITY: Belongs to the CYSTM1 family. {ECO:0000305}.
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DR EMBL; AC005662; AAM15069.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09972.1; -; Genomic_DNA.
DR EMBL; BT003101; AAO24533.1; -; mRNA.
DR PIR; T02437; T02437.
DR RefSeq; NP_181673.1; NM_129705.4.
DR AlphaFoldDB; Q8S8M0; -.
DR STRING; 3702.AT2G41420.1; -.
DR iPTMnet; Q8S8M0; -.
DR PaxDb; Q8S8M0; -.
DR PRIDE; Q8S8M0; -.
DR ProteomicsDB; 242656; -.
DR EnsemblPlants; AT2G41420.1; AT2G41420.1; AT2G41420.
DR GeneID; 818740; -.
DR Gramene; AT2G41420.1; AT2G41420.1; AT2G41420.
DR KEGG; ath:AT2G41420; -.
DR Araport; AT2G41420; -.
DR TAIR; locus:2040332; AT2G41420.
DR eggNOG; ENOG502S205; Eukaryota.
DR HOGENOM; CLU_128451_1_0_1; -.
DR InParanoid; Q8S8M0; -.
DR OMA; GYQGYQV; -.
DR PRO; PR:Q8S8M0; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q8S8M0; baseline and differential.
DR Genevisible; Q8S8M0; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; HDA:TAIR.
DR GO; GO:0009554; P:megasporogenesis; IGI:TAIR.
DR InterPro; IPR044850; WIH2-like.
DR PANTHER; PTHR31568; PTHR31568; 2.
PE 1: Evidence at protein level;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..98
FT /note="Cysteine-rich and transmembrane domain-containing
FT protein WIH2"
FT /id="PRO_0000365716"
FT TRANSMEM 75..92
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..77
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 7..41
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 53..73
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 98 AA; 10728 MW; 512A09DDDC3AFEC3 CRC64;
MSQYNQPPVG VPPPQGYPPE GYPKDAYPPQ GYPPQGYPQQ GYPPQGYPQQ GYPQQGYPPP
YAPQYPPPPQ HQQQQSSPGF LEGCLAALCC CCLLDACF
