ID   WIPI3_DANRE             Reviewed;         344 AA.
AC   Q7ZUW6;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=WD repeat domain phosphoinositide-interacting protein 3;
DE            Short=WIPI-3;
DE   AltName: Full=WD repeat-containing protein 45-like;
DE            Short=WDR45-like protein;
DE   AltName: Full=WD repeat-containing protein 45B;
GN   Name=wdr45b; Synonyms=wdr45l, wipi3;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=AB; TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Component of the autophagy machinery that controls the major
CC       intracellular degradation process by which cytoplasmic materials are
CC       packaged into autophagosomes and delivered to lysosomes for
CC       degradation. Binds phosphatidylinositol 3-phosphate (PtdIns3P), and
CC       other phosphoinositides including PtdIns(3,5)P2, forming on membranes
CC       of the endoplasmic reticulum upon activation of the upstream ULK1 and
CC       PI3 kinases and is recruited at phagophore assembly sites where it
CC       regulates the elongation of nascent phagophores downstream of WIPI2.
CC       {ECO:0000250|UniProtKB:Q5MNZ6}.
CC   -!- SUBCELLULAR LOCATION: Preautophagosomal structure
CC       {ECO:0000250|UniProtKB:Q5MNZ6}. Lysosome
CC       {ECO:0000250|UniProtKB:Q5MNZ6}.
CC   -!- DOMAIN: The L/FRRG motif is required for recruitment to PtdIns3P.
CC       {ECO:0000250|UniProtKB:Q9Y4P8}.
CC   -!- SIMILARITY: Belongs to the WD repeat PROPPIN family. {ECO:0000305}.
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DR   EMBL; BC047802; AAH47802.1; -; mRNA.
DR   AlphaFoldDB; Q7ZUW6; -.
DR   SMR; Q7ZUW6; -.
DR   STRING; 7955.ENSDARP00000126974; -.
DR   PaxDb; Q7ZUW6; -.
DR   ZFIN; ZDB-GENE-040426-863; wdr45b.
DR   eggNOG; KOG2111; Eukaryota.
DR   InParanoid; Q7ZUW6; -.
DR   PhylomeDB; Q7ZUW6; -.
DR   Reactome; R-DRE-1632852; Macroautophagy.
DR   PRO; PR:Q7ZUW6; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0019898; C:extrinsic component of membrane; IBA:GO_Central.
DR   GO; GO:0005764; C:lysosome; ISS:UniProtKB.
DR   GO; GO:0000407; C:phagophore assembly site; ISS:UniProtKB.
DR   GO; GO:0034045; C:phagophore assembly site membrane; IBA:GO_Central.
DR   GO; GO:0080025; F:phosphatidylinositol-3,5-bisphosphate binding; ISS:UniProtKB.
DR   GO; GO:0032266; F:phosphatidylinositol-3-phosphate binding; ISS:UniProtKB.
DR   GO; GO:0000045; P:autophagosome assembly; ISS:UniProtKB.
DR   GO; GO:0000422; P:autophagy of mitochondrion; IBA:GO_Central.
DR   GO; GO:0044804; P:autophagy of nucleus; IBA:GO_Central.
DR   GO; GO:0009267; P:cellular response to starvation; ISS:UniProtKB.
DR   GO; GO:0006497; P:protein lipidation; IBA:GO_Central.
DR   GO; GO:0034497; P:protein localization to phagophore assembly site; IBA:GO_Central.
DR   Gene3D; 2.130.10.10; -; 1.
DR   InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR   InterPro; IPR001680; WD40_repeat.
DR   InterPro; IPR036322; WD40_repeat_dom_sf.
DR   SMART; SM00320; WD40; 2.
DR   SUPFAM; SSF50978; SSF50978; 1.
PE   2: Evidence at transcript level;
KW   Autophagy; Lipid-binding; Lysosome; Reference proteome; Repeat; WD repeat.
FT   CHAIN           1..344
FT                   /note="WD repeat domain phosphoinositide-interacting
FT                   protein 3"
FT                   /id="PRO_0000051449"
FT   REPEAT          183..223
FT                   /note="WD 1"
FT   REPEAT          228..267
FT                   /note="WD 2"
FT   MOTIF           224..227
FT                   /note="L/FRRG motif"
FT                   /evidence="ECO:0000250|UniProtKB:Q9Y4P8"
SQ   SEQUENCE   344 AA;  38167 MW;  D7069B90588DC936 CRC64;
     MNLLPSNPHG NGLLYAGFNQ DHGCFACGME NGFRVYNTDP LKEKEKHEFL EGGVGHVEML
     FRCNYLALVG GGKKPKYPPN KVMIWDDLKK KTVIEIEFST EVKAVKLRRD RIVVVLDSMI
     KVFTFTHNPH QLHVFETCYN PKGLCVLCPN SNNSLLAFPA THSGHVQIVD LANTEKPPVD
     IPAHEGVLCC ITLNLQGTRI ATASEKGTLI RIFDTSAGQL IQELRRGSQT ANIYCINFNQ
     DASLICVSSD HGTVHIFAAE DPKRNKQSSL ASASFLPKYF SSKWSFSKFQ VPSGSPCVCA
     FGTEPNAVIA ICADGSYYKF LFNPKGECSR DVYAQFLEMT DEKI